Qhov sib txawv tseem ceeb ntawm calibration nkhaus absorbance thiab concentration yog tias calibration nkhaus yog ib daim duab ntawm absorbance thiab concentration, absorbance yog tus nqi ntawm lub teeb absorbed los ntawm ib tug qauv whereas concentration yog tus nqi ntawm cov khoom faib nyob rau hauv ib chav tsev ntim.
Spectroscopy yog ib qho kev tshuaj ntsuam xyuas uas muaj txiaj ntsig zoo hauv kev txiav txim siab ntawm qhov tsis paub qhov sib txuas tam sim no hauv cov qauv muab. Yog li ntawd, nws yog ib qho kev soj ntsuam quantitative. Hauv cov txheej txheem no, peb tuaj yeem txiav txim siab qhov concentration ntawm qhov sib xyaw siv qhov nkhaus. Peb yuav tsum kos qhov nkhaus ntawm absorbance thiab concentration. Thiab peb tuaj yeem kos daim duab rau ntau qhov txiaj ntsig absorbance tau rau qhov sib txawv paub qhov tseem ceeb. Tom qab ntawd, peb tuaj yeem siv tus nqi nqus rau cov qauv uas tsis paub los txiav txim siab qhov concentration ntawm cov qauv ntawd siv daim duab.
Calibration Curve yog dab tsi?
A calibration nkhaus yog cov qauv duab uas qhia tau hais tias qhov kev hloov pauv hauv cov lus teb ntawm qhov ntsuas ntsuas ntawm qhov sib txawv ntawm qhov ntsuas. Analyte yog cov khoom uas peb yuav tsum tau nrhiav qhov concentration. Yuav kom kos cov calibration nkhaus, peb yuav tsum siv qhov paub txog cov khoom muaj nyob hauv peb cov qauv kom tau txais cov lus teb lossis cov teeb liab. Rau cov txheej txheem spectroscopic, cov lus teb lossis cov cim qhia yog qhov tseem ceeb absorbance. Tom qab ntawd, peb tuaj yeem kos ib daim duab ntawm absorbance thiab concentration.
Daim duab 01: Tus Qauv ntawm Kev Ntsuas Ntsuas
Peb yuav tsum kos daim duab siv cov txiaj ntsig absorbance tau rau txhua qhov kev paub paub. Peb tuaj yeem siv daim duab no los txiav txim siab qhov tsis paub txog qhov sib xyaw ua ke hauv ib qho piv txwv los ntawm kev ntsuas qhov nqus ntawm cov qauv ntawd. Tus nqi concertation ntawm qhov point ntawm tus nqi absorbance nyob rau hauv lub nkhaus yog qhov concentration ntawm cov compound nyob rau hauv tus qauv.
Dab tsi yog Absorbance?
Absorbance yog cov lus teb ntawm spectrophotometer rau qhov concentration ntawm cov qauv. Nws ntsuas tus nqi ntawm lub teeb absorbs los ntawm cov qauv. Tus nqi no nyob ntawm tus nqi thiab qhov xwm txheej ntawm cov khoom sib xyaw uas muaj nyob hauv cov qauv. Peb tuaj yeem muab tus nqi no siv qhov sib npaug hauv qab no;
A=log(I/I0)
Qhov twg A yog qhov nqus, Kuv yog qhov hnyav ntawm qhov teeb meem kab teeb meem, thiab kuv oyog qhov kev siv ntawm cov nqaj kis los ntawm cov qauv. Peb tuaj yeem muab qhov kev sib raug zoo no rau lwm txoj hauv qab no:
A=– logT
T yog qhov chaw xa khoom. Yog li ntawd, lub absorbance muaj feem xyuam rau lub transmittance. rau tib yam khoom, yog hais tias lub concertation siab, lub absorbance kuj siab thiab vice versa. Tias yog vim li cas, yog tias qhov concentration siab, tus qauv muaj ntau qhov sib xyaw uas absorbs lub teeb los ntawm lub teeb beam. Ntxiv mus, thaum ntsuas qhov absorbance los ntawm lub spectrophotometer, peb yuav tsum tsis txhob siv siab heev los yog tsawg concentrations. Qhov no yog vim hais tias, yog tias peb siv cov concentrations siab heev, qhov kev siv ntawm qhov teeb meem teeb pom kev zoo yuav tsis txaus rau tag nrho cov khoom xyaw tam sim no hauv cov qauv kom nqus tau. Yog tias peb siv cov concentration tsawg, qhov rhiab heev ntawm qhov ntsuas yuav tsis txaus txaus los kuaj xyuas qhov qis ntawm cov khoom sib xyaw hauv cov qauv.
Kev Cuam Tshuam yog dab tsi?
Kev sib xyaw yog tus nqi ntawm cov khoom uas faib thoob plaws hauv ib chav tsev ntawm cov qauv. Peb tuaj yeem ntsuas qhov no hauv chav tsev ntawm mol / L uas peb muab cov khoom siv raws li tus nqi mole thiab ntim ntawm cov qauv hauv litres. Peb hu qhov no lub molar concentration. Nws yog chav tsev ntau tshaj plaws rau kev ntsuas qhov concentration.
Dhau li ntawm qhov ntawd, peb tuaj yeem txiav txim siab qhov kev hais kwv txhiaj raws li huab hwm coj concentration, tus lej concentration lossis qhov ntim concentration.
Kev sib raug zoo ntawm Calibration Curve Absorbance thiab Concentration yog dab tsi?
Peb kos qhov nkhaus nkhaus los ntawm ob cov ntaub ntawv teev: absorbance qhov tseem ceeb thiab cov concentrations. Peb yuav tsum coj lub absorbance li y-axis thiab concentration li x-axis vim peb tuaj yeem hloov tus nqi ntawm qhov concentration. Li no, nws yog tus hloov pauv ywj pheej. Tab sis lub absorbance hloov raws li tus nqi ntawm cov concentration. Yog li ntawd, nws yog qhov txawv txav. Tom qab ntawd, yog tias peb ntsuas qhov nqus ntawm tus qauv, peb tuaj yeem tau txais qhov concentration ntawm tus qauv siv qhov kev ntsuas qhov ntsuas no.
Qhov txawv ntawm Calibration Curve Absorbance thiab Concentration yog dab tsi?
A calibration nkhaus yog cov qauv duab uas qhia tau hais tias qhov kev hloov pauv hauv cov lus teb ntawm qhov ntsuas ntsuas ntawm qhov sib txawv ntawm qhov ntsuas. Nws qhia txog absorbance hauv y-axis thiab concentration hauv x-axis. Absorbance yog cov lus teb ntawm spectrophotometer rau qhov concentration ntawm cov qauv. Nws tsis muaj units. Concentration yog tus nqi ntawm cov khoom uas faib thoob plaws hauv ib chav tsev ntawm cov qauv. Nws chav tsev yog mol/L.
Summary – Calibration Curve Absorbance vs Concentration
Calibration curves, absorbance thiab concentration, yog siv dav hauv spectroscopy. Qhov sib txawv ntawm calibration nkhaus absorbance thiab concentration yog qhov calibration nkhaus yog ib daim duab ntawm absorbance thiab concentration thiab absorbance yog tus nqi ntawm lub teeb absorbed los ntawm ib tug qauv whereas concentration yog tus nqi ntawm cov khoom faib nyob rau hauv ib chav tsev ntim.
