Qhov sib txawv tseem ceeb ntawm Michaelis Menten thiab Lineweaver Burk zaj dab neeg yog tias Michaelis Menten cov phiaj xwm muab kev kwv yees ntau dua ntawm Vmax thiab cov ntaub ntawv raug ntau dua txog kev txwv tsis pub tshaj Lineweaver Burk zaj dab neeg.
Michaelis Menten thiab Lineweaver Burk cov phiaj xwm yog ob qho qauv tseem ceeb hauv kev tshuaj ntsuam chemistry thiab hauv biochemistry vim tias lawv pab tau txiav txim siab enzyme kinetics.
Michaelis Menten Plot yog dab tsi?
Michaelis Menten zaj duab xis tuaj yeem piav qhia raws li tus qauv muaj txiaj ntsig hauv kev piav qhia tus nqi ntawm cov tshuaj tiv thaiv enzymatic. Qhov no yog ib qho ntawm cov qauv zoo tshaj plaws ntawm cov enzyme kinetics. Tus qauv tau muab npe tom qab German biochemist Leonor Michaelis thiab Canadian tus kws kho mob Maud Menten. Michaelis Menten zaj dab neeg ua haujlwm los ntawm kev hais txog cov tshuaj tiv thaiv rau qhov concentration ntawm substrate.
Michaelis Menten sib npaug tuaj yeem muab tau raws li hauv qab no:
V=d[P]/dt=Vmax ([S]/KM + [S])
Hauv qhov sib npaug no, Vmax yog hais txog qhov siab tshaj plaws uas ua tiav los ntawm lub kaw lus, uas tshwm sim ntawm qhov saturating substrate concentration rau ib qho tshwj xeeb enzyme concentration. Ntawm qhov point uas tus lej tus nqi ntawm KM yog sib npaug rau cov substrate concentration, cov tshuaj tiv thaiv ua ib nrab ntawm Vmax
Lineweaver Burk Plot yog dab tsi?
Lineweaver Burk zaj duab xis tuaj yeem piav qhia raws li cov duab sawv cev ntawm Lineweaver Burk kab zauv ntawm enzyme kinetics. Qhov tshwm sim no tau piav qhia thawj zaug los ntawm Hans Lineweaver thiab Dean Burk hauv xyoo 1934. Cov phiaj xwm no yog qhov tseeb thaum cov enzyme kinetics ua raws li qhov kev txiav txim thib ob ntawm kinetics. Tab sis tsis yog-linear regression yog xav tau rau cov tshuab uas tsis coj tus cwj pwm zoo. Tsis tas li ntawd, ob chav sib txuas lus sib cuam tshuam cuam tshuam cov qauv kev ua yuam kev ntawm cov ntaub ntawv. Yog li, nws tsis yog qhov tseeb tshaj plaws rau kev txiav txim siab ntawm enzyme kinetic tsis. Peb tuaj yeem siv qhov sib npaug Michaelis Menten hauv nws qhov tsis yog-linear regression lossis lwm hom kab ntawv rau kev suav cov tsis.
Peb tuaj yeem siv hom phiaj no rau kev txiav txim siab ntawm enzyme inhibition. Ntawm no, cov phiaj xwm no pab kom paub qhov txawv ntawm kev sib tw, tsis sib tw, thiab tsis sib tw inhibitors. Siv cov qauv no, peb kuj tuaj yeem sib piv ntau hom kev tiv thaiv nrog cov tshuaj tiv thaiv tsis muaj zog.
Txawm hais tias zaj dab neeg no tseem ceeb hauv kev txiav txim siab ntawm qhov hloov pauv hauv enzyme kinetics, nws qhia qhov yuam kev. Piv txwv li, y-axis ntawm zaj duab xis siv qhov sib txuam ntawm tus nqi ntawm cov tshuaj tiv thaiv. Qhov no txhais tau hais tias qhov yuam kev me me hauv kev ntsuas tau pom ntau dua ntawm no. Tsis tas li ntawd, cov txiaj ntsig tau los ntawm qhov qis substrate concentration yog nyob rau sab xis ntawm daim phiaj. Qhov no muaj kev cuam tshuam loj dua ntawm txoj kab nqes hav; yog li ntawd, nws tshwj xeeb muaj feem cuam tshuam rau tus nqi Km.
Qhov txawv ntawm Michaelis Menten thiab Lineweaver Burk Plot yog dab tsi?
Michaelis Menten zaj duab xis yog tus qauv siv tau los piav txog tus nqi ntawm cov tshuaj tiv thaiv enzymatic, thaum Lineweaver Burk zaj duab xis yog cov duab sawv cev ntawm Lineweaver Burk kab zauv ntawm enzyme kinetics. Qhov sib txawv tseem ceeb ntawm Michaelis Menten thiab Lineweaver Burk zaj dab neeg yog tias Michaelis Menten cov phiaj xwm muaj tseeb dua hauv kev muab cov ntaub ntawv hais txog kev txwv tsis pub tshaj Lineweaver Burk zaj dab neeg thiab muab qhov kwv yees zoo dua ntawm Vmax.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm Michaelis Menten thiab Lineweaver Burk cov phiaj xwm hauv daim ntawv teev npe rau kev sib piv ntawm ib sab.
Summary – Michaelis Menten vs Lineweaver Burk Plot
Michaelis Menten thiab Lineweaver Burk cov phiaj xwm yog ob qho qauv tseem ceeb hauv kev tshuaj ntsuam chemistry thiab hauv biochemistry vim lawv pab txiav txim siab enzyme kinetics. Qhov sib txawv tseem ceeb ntawm Michaelis Menten thiab Lineweaver Burk zaj dab neeg yog lawv qhov tseeb. Thaum piv rau Lineweaver Burk zaj dab neeg, Michaelis Menten cov phiaj xwm muab kev kwv yees ntau dua ntawm Vmax thiab cov ntaub ntawv raug ntau ntxiv txog enzyme inhibition.
