Qhov sib txawv tseem ceeb ntawm kev txheeb xyuas metabolic flux thiab flux tshuav nyiaj li cas yog qhov kev tsom xam cov tshuaj metabolic flux yog cov txheej txheem kev tshuaj xyuas fluxomics rau kev tshuaj xyuas kev tsim khoom thiab kev noj cov nqi ntawm cov metabolites hauv cov kab ke lom neeg, thaum ntsuas qhov sib npaug yog cov txheej txheem ua lej fluxomics rau kev tshuaj xyuas. Kev khiav dej num ntawm metabolites los ntawm lub network metabolic.
Fluxomics yog hais txog ntau txoj hauv kev siv los txiav txim siab tus nqi ntawm cov tshuaj metabolic hauv cov kab mob lom. Qhov tseem ceeb ntawm fluxomics yog tias metabolic fluxes feem ntau txiav txim siab txog cellular phenotypes. Metabolic flux yog kev hloov pauv hauv cov metabolite hauv lub network metabolic. Muaj ob qhov tseem ceeb fluxomics lossis flux tsom technologies. Lawv yog metabolic flux tsom xam thiab flux tshuav nyiaj li cas.
Kev Ntsuas Metabolic Flux yog dab tsi?
Metabolic flux tsom xam (MFA) yog cov txheej txheem kev tshuaj xyuas cov tshuaj fluxomics uas tshuaj xyuas cov khoom tsim thiab kev noj cov tshuaj metabolites hauv cov kab mob lom. Cov txheej txheem no tso cai rau kom muaj nuj nqis ntawm cov metabolites ntawm qib intracellular. Yog li ntawd, cov txheej txheem no elucidates lub hauv paus metabolism ntawm lub cell. Hauv kev txheeb xyuas metabolic flux,13 C fluxomics raws li txoj kev feem ntau yog siv rau kev tshuaj xyuas flux. Hauv 13C fluxomics, cov metabolic precursors yog enriched nrog 13C ua ntej nkag mus rau hauv cov kab mob lom. Tom qab ntawd, siv cov tswv yim kev yees duab xws li huab hwm coj spectrometry thiab nuclear magnetic resonance spectroscopy, theem ntawm kev koom ua ke ntawm 13 C rau hauv cov metabolites yog ntsuas.
Daim duab 01: Kev Ntsuas Metabolic Flux
Ntxiv rau, cov kab mob metabolic tuaj yeem ntsuas nrog stoichiometry. Hauv kev txheeb xyuas metabolic flux, cov qauv stoichiometric thiab cov txheej txheem loj spectrometry txiav txim siab hloov cov moieties uas muaj isotopic tracers los ntawm ib qho metabolite mus rau lwm qhov hauv lub network metabolic. Yog li, cov txheej txheem no tuaj yeem muab cov ntaub ntawv hais txog tag nrho cov metabolic network thiab cellular phenotype. Tsis tas li ntawd, kev txheeb xyuas cov tshuaj metabolic flux muaj ntau yam kev siv. Cov txheej txheem no tuaj yeem txiav txim siab qhov txwv ntawm lub peev xwm ntawm cov kab mob lom neeg hauv kev tsim cov tshuaj biochemical xws li ethanol. Ntxiv rau qhov ntawd, cov txheej txheem no tuaj yeem kwv yees cov lus teb rau cov noob ntxiv los yog knockouts.
Flux Balance Analysis yog dab tsi?
Flux balance analysis (FBA) yog ib txoj kev ua lej fluxomics uas txheeb xyuas cov dej ntws ntawm cov metabolites los ntawm lub network metabolic. Nws yog ua tiav ntawm ob kauj ruam txheej txheem. Hauv thawj kauj ruam, cov tshuaj tiv thaiv metabolic tau sawv cev ua lej nyob rau hauv daim ntawv ntawm tus lej matrix uas muaj stoichiometric coefficients ntawm txhua qhov tshuaj tiv thaiv. Cov stoichiometric coefficients ua rau muaj kev txwv ntawm cov qauv kev ua haujlwm. Los ntawm muab cov kev txwv zoo li no, cov txheej txheem kev daws teeb meem tau raug txo qis. Hauv cov kauj ruam thib ob, ua raws li cov kev txwv ntxiv, cov qauv kev ua haujlwm tau zoo. Flux tshuav nyiaj li cas xam cov dej ntws ntawm cov metabolites hauv lub network metabolic los ntawm kev siv cov txheej txheem saum toj no. Li no, nws muaj peev xwm kwv yees qhov kev loj hlob ntawm cov kab mob los yog tus nqi tsim tawm ntawm biotechnologically tseem ceeb metabolite los ntawm FBA.
Daim duab 02: Flux Balance Analysis
Cov peev txheej rau kev txheeb xyuas qhov sib npaug suav nrog BIGG database, COBRA toolbox, thiab FASIMU (FBA simulation software metabolomics). Flux tshuav nyiaj li cas tsom xam muaj ntau yam kev siv nyob rau hauv physiological kev tshawb fawb, qhov sib txawv filling dag zog, thiab genome-scale hluavtaws biology. Tsis tas li ntawd, FBA kuj tuaj yeem siv los kwv yees cov txiaj ntsig ntawm cov khoom tseem ceeb xws li ATP, NADH, lossis NADPH.
Similarities ntawm Metabolic Flux Analysis thiab Flux Balance Analysis
- Metabolic flux analysis thiab flux tshuav nyiaj li cas yog flux tsom technologies.
- Ob txoj kev poob rau hauv thaj chaw ntawm kev ua haujlwm biology
- Cov tswv yim no tuaj yeem txiav txim siab cov kab mob metabolic hauv cov kab mob lom.
- Cov txheej txheem no muaj kev siv dav.
- Ob txoj kev tuaj yeem txiav txim siab ntawm cell phenotype.
Qhov Sib Txawv Ntawm Kev Ntsuas Metabolic Flux thiab Flux Balance Analysis
Metabolic flux tsom xam yog ib qho kev ntsuas fluxomics cov txheej txheem siv los txheeb xyuas kev tsim khoom thiab kev siv tus nqi ntawm cov metabolites hauv cov kab ke lom neeg, thaum kev txheeb xyuas qhov sib npaug flux yog cov txheej txheem ua lej fluxomics uas txheeb xyuas cov dej ntws ntawm cov metabolites los ntawm lub network metabolic. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev txheeb xyuas metabolic flux thiab flux tshuav nyiaj li cas. Tsis tas li ntawd, kev txheeb xyuas cov tshuaj metabolic flux siv cov spectrometry loj lossis nuclear sib nqus resonance los txiav txim siab cov metabolic fluxes, thaum cov flux tshuav nyiaj li cas siv cov lej (tus lej) matrix los txiav txim siab metabolic fluxes.
Cov infographic hauv qab no teev qhov sib txawv ntawm kev tsom xam cov tshuaj metabolic flux thiab flux tshuav nyiaj li cas hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Metabolic Flux Analysis vs Flux Balance Analysis
Flux tsom xam (fluxomics) thev naus laus zis yog siv los txiav txim siab tus nqi ntawm cov tshuaj tiv thaiv metabolic hauv cov kab mob lom. Muaj ob lub ntsiab flux tsom xam technologies. Lawv yog cov tshuaj ntsuam xyuas metabolic flux thiab flux tshuav nyiaj li cas. Metabolic flux tsom xam yog ib qho kev ntsuam xyuas cov txheej txheem fluxomics rau kev tshuaj xyuas kev tsim khoom thiab kev noj cov nqi ntawm cov metabolites hauv cov kab ke lom neeg, thaum kev txheeb xyuas qhov sib npaug flux yog lej fluxomics txheej txheem rau kev tshuaj xyuas cov dej ntws ntawm cov metabolites los ntawm lub network metabolic. Yog li, qhov no qhia txog qhov sib txawv ntawm kev tsom xam cov tshuaj metabolic flux thiab flux tshuav nyiaj li cas.
