Qhov Tseem Ceeb Qhov txawv - Inoculation vs Incubation
Cov kab mob kis tau raug coj mus rau hauv chav kuaj thiab kev lag luam rau ntau lub hom phiaj xws li kev ua tus cwj pwm, kev sib txawv, kev txheeb xyuas, kev tsim cov tshuaj tua kab mob, kev tsim cov tshuaj tiv thaiv, kev tsim cov nroj tsuag thiab tsiaj txhu transgenic (GMO), thiab tshem tawm cov organic acids. Lawv loj hlob nyob rau hauv cov khoom siv hluavtaws loj hlob los yog hauv cov substrates. Yog li ntau hom kev tsis huv tshiab yuav tsum tau npaj, thiab cov kab mob uas xav tau yog kab lis kev cai hauv cov kab lis kev cai ntshiab lossis sib xyaw. Media yog supplemented nrog tag nrho cov as-ham tsim nyog rau kev loj hlob ntawm cov kab mob. Qhov kev txiav txim ntawm kev qhia cov kab mob mus rau hauv cov nruab nrab tshiab los yog substrate yog hu ua inoculation. Txawm li cas los xij, qhov zoo tshaj plaws kev loj hlob yuav tsum tau muab rau kom ua tiav qhov kev loj hlob txaus ntawm cov kab mob. Cov txheej txheem ntawm kev muab cov kev loj hlob uas yuav tsum tau muaj xws li qhov kub thiab txias, noo noo, thiab pH thiab tso cai rau cov kab mob loj hlob ntawm cov xov xwm hu ua incubation. Yog li, qhov sib txawv tseem ceeb ntawm inoculation thiab incubation yog qhov inoculation yog kev qhia cov kab mob mus rau cov xov xwm loj hlob los yog substrates thaum incubation tso cai rau cov kab mob loj hlob nyob rau hauv kev loj hlob cov kev mob.
Dab tsi yog Inoculation?
Inoculation yog txheej txheem ntawm kev qhia cov kab mob mus rau hauv qhov nruab nrab loj hlob uas tsim nyog rau lawv txoj kev loj hlob. Hauv lwm lo lus, inoculation tuaj yeem txhais tau tias yog cov txheej txheem uas qhia txog cov kab mob pathogenic lossis antigenic microorganism rau hauv cov kab mob uas muaj sia nyob txhawm rau txhawm rau txhawb kev tsim cov tshuaj tiv thaiv. Thaum lub inoculation tiav, cov kab mob pib loj hlob thiab muab faib rau hauv nruab nrab los ntawm kev tsim cov colony pom.
Muaj ntau hom inoculation cov cuab yeej thiab cov tswv yim siv hauv microbiology. Inoculating voj, inoculating koob, paj rwb swabs, forceps, iav preaders, dispenser pipettes yog cov feem ntau siv inoculation cuab yeej nyob rau hauv lub chaw soj nstuam. Tag nrho cov ntaub ntawv no yuav tsum tsis pub muaj kab mob. Yog li ntawd, ua ntej inoculation, nws yog ib qho tsim nyog yuav tsum tau ua kom tsis muaj menyuam siv cov txheej txheem kom tsis muaj menyuam kom tsis txhob muaj kab mob los yog kev loj hlob ntawm cov kab mob tsis zoo hauv kab lis kev cai. Streak phaj txoj kev, kis qhov chaw txoj kev, ncuav phaj txoj kev, taw tes inoculation, stab kab lis kev cai, slant kab lis kev cai yog ob peb inoculating cov tswv yim siv nyob rau hauv microbial laboratories kom loj hlob cov kab mob thiab fungi.
Daim duab 01: Inoculation ntawm cov kab mob siv cov txheej txheem streak phaj
Yuav ua li cas yog dab tsi?
Cov kab mob microorganisms muaj cov kev loj hlob sib txawv. Lawv yuav tsum tau muab cov as-ham uas xav tau, dej, minerals, loj hlob yam, kab kawm thiab lwm yam kev loj hlob. Tom qab inoculating ib microbe mus rau hauv ib tug tshiab nruab nrab, loj hlob tej yam kev mob yuav tsum tau tswj kom txhawb kev loj hlob ntawm cov kab mob. Cov txheej txheem tso cai rau cov kab mob loj hlob hauv nruab nrab los ntawm kev muab cov kev loj hlob tsim nyog yog hu ua incubation. Inoculated kab lis kev cai daim hlau tuaj yeem muab tso rau hauv ib lub cuab yeej hu ua incubator rau incubation. Incubators yog tsim los ntawm txoj kev uas tus neeg teb xov tooj tuaj yeem tswj qhov kub, av noo, roj concentration, thiab lwm yam raws li qhov xav tau ntawm microbe.
Dab tsi yog Phase hauv Microbial Loj hlob?
Thaum qhov kev pom zoo tau muab, cov kab mob me me yuav loj hlob, tsim tawm thiab sib npaug los ntawm kev siv cov khoom noj muaj nyob hauv nruab nrab. Kev loj hlob ntawm microbial muaj plaub theem sib txawv hauv cov kab lis kev cai nruab nrab. Tom qab inoculation, lawv pib lub lag luam theem. Thaum lub sij hawm lag luam, cov kab mob microbes tsis pom kev loj hlob sai lossis sib npaug. Lawv pib hloov mus rau qhov chaw tshiab thiab ruaj khov nyob ntawd. Thaum lawv hloov kho, theem thib ob, uas qhia txog kev loj hlob ntawm cov kab mob, pib. Theem ob yog hu ua log theem lossis exponential theem. Thaum lub sij hawm log, microbes qhia pom kev loj hlob thiab kev sib npaug. Qhov thib peb theem pib tom qab lub log theem thaum cov as-ham thiab lwm yam yuav tsum tau txwv nyob rau hauv nruab nrab. Thaum lub sij hawm nyob ruaj ruaj, kev loj hlob thiab kev tuag yuav sib npaug, thiab kev loj hlob nkhaus yog nyob rau hauv ib txoj kab ncaj nraim mus rau lub x-axis. Qib plaub yog theem kev tuag uas qhov kev tuag ntau tshaj qhov kev loj hlob. Tom qab ob peb hnub, microbial kev loj hlob tsis tu ncua, tawm hauv kab lis kev cai tuag.
Daim duab 02: Microbial phaj incubator
Qhov txawv ntawm Inoculation thiab Incubation yog dab tsi?
Inoculation vs Incubation |
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| Inoculation yog txheej txheem ntawm kev qhia cov kab mob lossis tshem tawm cov kab mob mus rau hauv kab lis kev cai nruab nrab. | Incubation yog tus txheej txheem uas tso cai rau cov kab mob inoculated kom loj hlob raws li qhov yuav tsum tau muaj kev loj hlob. |
| Tswv siv | |
| Inoculation tuaj yeem ua tiav siv cov koob txhaj tshuaj, inoculating loops, paj rwb swaps, pipettes, thiab lwm yam. | Txhim kho tuaj yeem ua tiav hauv chav kab lis kev cai, chaw tsim khoom, kab lis kev cai racks, thiab lwm yam. |
| Inoculation tau ua tiav hauv lub sijhawm luv. | Incubation siv sij hawm ntau teev rau hnub. |
| Inoculation yog ua tiav nyob rau hauv aseptic tej yam kev mob nyob rau hauv lub laminar cua txee. | Incubation yog ua los ntawm kev muab kev loj hlob zoo xws li kub, av noo, oxygen concentration, lub teeb, thiab lwm yam. |
Summary – Inoculation vs Incubation
Inoculation thiab incubation yog ob kauj ruam tseem ceeb koom nrog hauv kev tsim cov kab mob hauv cov chaw sim. Inoculation yog qhov kev txiav txim ntawm kev qhia cov kab mob mus rau cov kab lis kev cai tsim nyog nruab nrab lossis substrate. Inoculated media yog muab nrog cov xwm txheej tsim nyog kom loj hlob thiab sib npaug. Cov txheej txheem no hu ua incubation. Qhov no yog lub ntsiab sib txawv ntawm inoculation thiab incubation. Muaj cov cuab yeej tshwj xeeb thiab cov khoom siv hauv cov chaw kuaj kab mob microbial rau incubation lub hom phiaj. Lub incubator yog ib yam khoom uas tso cai rau cov kab mob loj hlob nyob rau hauv kev tswj qhov kub thiab txias, aeration, humidity, thiab lwm yam. Inoculation thiab incubation yuav tsum tau ua raws li aseptic tej yam kev mob kom tsis txhob muaj kab mob thiab nkim sij hawm.
Download PDF Version of Inoculation vs Incubation
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li cov ntawv sau tseg. Thov download PDF version ntawm no Qhov txawv Ntawm Inoculation thiab Incubation.
