Agarose vs Polyacrylamide
Agarose thiab Polyacrylamide yog ob qho tib si dej-soluble polymers tab sis, ntawm lawv, ntau qhov sib txawv tuaj yeem pom, pib los ntawm lawv cov keeb kwm. Ob leeg Agarose thiab Polyacrylamide muaj qee yam hauv lawv lub peev xwm los tsim cov gel matrices porous. Txawm li cas los xij, muaj ntau qhov sib txawv ntawm ob qho tib si. Qhov sib txawv loj ntawm ob qho tib si ntawm cov polymers nyob rau hauv lawv qhov chaw ntawm keeb kwm, tshuaj qauv, lawv siv sib txawv, thiab lawv cov kev ua tau zoo ntawm gel electrophoresis.
Agarose yog dab tsi?
Agarose yog ib txwm tshwm sim linear polymer uas yog nyob rau hauv lem muab los ntawm ib tug complex polymer hu ua agar pom nyob rau hauv seaweed. Agarose yog muab rho tawm los ntawm agar los ntawm kev tshem tawm ntawm nws cov protein tivthaiv hu ua agaropectin. Agarose yog dab tsi muab agar nws muaj peev xwm tsim gels.
Kev siv loj ntawm agarose yog hauv kev tshawb fawb microbiological thiab molecular biological. Hauv kev tshawb fawb microbiological, agarose, thaum ntxiv nrog cov as-ham tsim nyog, muab lub hauv paus ruaj khov rau kev cog cov kab mob xws li kab mob thiab fungi. Thaum siv nyob rau hauv ib nrab-khoom concentrations, nws yuav pab tau nyob rau hauv kev ntsuam xyuas lub motility ntawm cov kab mob no. Hauv molecular biology, nws yog ib qho cuab yeej tseem ceeb rau ib qho kev daws teeb meem tseem ceeb tshaj plaws hu ua 'gel electrophoresis' lossis 'agarose gel electrophoresis' (AGE). Gel electrophoresis yog txheej txheem uas tso cai rau kev daws teeb meem lossis kev sib cais ntawm nucleic acids lossis protein raws li lawv qhov loj thiab nqi. Ntawm no, agarose ua hauj lwm raws li ib tug ntxeem tau sieve-zoo li gel los ntawm kev sib cais tshwm sim.
Agarose Structure
YPolyacrylamide yog dab tsi?
Polyacrylamide yog cov khoom siv hluavtaws thiab siv rau ntau yam kev lag luam. Raws li tau hais dhau los, nws txoj kev siv yog nyob ntawm nws lub peev xwm los tsim cov gels. Txawm li cas los xij, ntxiv rau qhov no, nws muaj peev xwm khaws cia thiab tso dej ntawm qhov sib txawv ntawm qhov sib txawv kuj yog siv rau hauv ntau qhov kev lag luam.
Kev siv ntau tshaj plaws ntawm polyacrylamide yog hauv kev kho dej khib nyiab. Ntawm no, nws yog siv los ua tus neeg sawv cev flocculating kom tshem tawm cov khoom siv uas raug tshem tawm; li no, txhim kho turbidity thiab clarifying dej. Lwm qhov kev siv ntawm polyacrylamide yog nyob rau hauv daim ntawv kev lag luam. Ntawm no, nws yog siv los khaws lossis tso dej los ntawm daim ntawv pulp raws li xav tau. Ib yam li ntawd, hauv kev ua liaj ua teb thiab kev tsim kho kev lag luam, nws yog siv los ua cov av txias kom tiv thaiv av yaig thiab txhim kho nws qhov zoo.
Zoo li agarose, polyacrylamide ib yam nkaus yog siv hauv molecular biology ua cov cuab yeej daws teeb meem tseem ceeb hauv cov txheej txheem zoo sib xws hu ua 'Polyacrylamide gel electrophoresis' (PAGE). Ntxiv rau tag nrho cov no, Polyacrylamide kuj tseem siv rau hauv kev ua cov ore thiab kev tsim cov flocculating tus neeg saib xyuas kom tshem tawm cov khoom siv organic; li no, txhim kho turbidity thiab clarifying dej. Lwm qhov kev siv ntawm polyacrylamide yog nyob rau hauv daim ntawv kev lag luam. Ntawm no, nws yog siv los khaws lossis tso dej los ntawm daim ntawv pulp raws li xav tau. Ib yam li ntawd, nyob rau hauv kev ua liaj ua teb thiab kev tsim kho kev lag luam, nws yog siv los ua cov av txias los tiv thaiv cov av yaig thiab txhim kho nws qhov zoo. Ntxiv rau tag nrho cov no, Polyacrylamide kuj tseem siv rau hauv kev tsim cov khoom noj khoom haus ntxiv, cov tshuaj tiv thaiv mos, thiab textiles.
Polyacrylamide Structure
Qhov txawv ntawm Agarose thiab Polyacrylamide yog dab tsi?
Keeb kwm ntawm Agarose thiab Polyacrylamide:
Agarose: Agarose yog ib qho polymer ntawm ntuj tsim. Nws yog muab los ntawm seaweed.
Polyacrylamide: Polyacrylamide yog cov khoom siv hluavtaws thiab tsis pom nyob rau hauv ib qho xwm txheej ntuj.
Molecular Formula ntawm Agarose thiab Polyacrylamide:
Agarose: Cov mis molecular ntawm agarose yog C24H38O19.
Polyacrylamide: Cov mis molecular ntawm polyacrylamide yog (C 3 H 5 NO) n.
Cov Qauv Tshuaj ntawm Agarose thiab Polyacrylamide:
Agarose: Agarose yog ib qho linear polysaccharide. Nws yog tsim los ntawm kev rov ua cov disaccharide units hu ua agrobiose tuav ua ke los ntawm hydrogen bonds.
Polyacrylamide: Polyacrylamide yog ib qho tshuaj sib txuas ntawm cov polymer. Nws yog tsim los ntawm acrylamide monomers thiab crosslinking tus neeg sawv cev N, N'-methylenebisacrylamide.
toxicity ntawm Agarose thiab Polyacrylamide:
Agarose: Ob qho agarose thiab nws lub tsev monomer agrobiose tsis muaj tshuaj lom nyob rau hauv qhov xwm txheej.
Polyacrylamide: Lub monomer chav tsev ntawm polyacrylamide, lub acrylamide, yog ib tug presumed carcinogen thiab paub neurotoxin thaum nws yog polymerised daim ntawv yog non-toxic nyob rau hauv cov xwm.
yam ntxwv ntawm Agarose thiab Polyacrylamide Gels:
AGE thiab PAGE:
Agarose: Agarose gel npaj rau AGE yog siv sijhawm tsawg dua, yooj yim thiab yooj yim, thiab tsis tas yuav tsum muaj tus pib lossis polymerising catalyst.
Polyacrylamide: Polyacrylamide gel npaj rau PAGE yog siv sijhawm ntev thiab nkees thiab tseem xav tau tus pib (ammonium persulphate) thiab polymerising catalyst (N, N, N ', N'-tetramethylethylendiamine - TEMED).
Nature:
Polyacrylamide gels muaj tshuaj ruaj khov dua li agarose gels.
Pore Size:
Muab tib qhov concentration, polyacrylamide gel matrices zoo li yuav muaj qhov pore me me piv rau ntawm agarose gel matrix.
Altering Pore Size:
Qhov pore loj ntawm polyacrylamide gels tuaj yeem hloov pauv raws li kev tswj hwm ntau dua li ntawm agarose gels.
Kev daws lub zog:
Polyacrylamide gels muaj lub zog daws teeb meem thaum agarose gels muaj lub zog daws teeb meem tsawg.
Accommodating Nucleic Acid:
Polyacrylamide gels tuaj yeem haum cov nucleic acid ntau dua li agarose gels rau kev daws teeb meem.
