Qhov sib txawv tseem ceeb ntawm agarose thiab polyacrylamide gel electrophoresis yog agarose gel electrophoresis siv kab rov tav ncuav agarose gels cais qhov sib piv loj dua ntawm DNA, thaum polyacrylamide gel electrophoresis siv vertically poured polyacrylamide gels cais luv luv nucleic acid.
Electrophoresis yog ib hom kev siv hluav taws xob siv thoob plaws hauv gel matrix los cais cov biomolecules xws li DNA, RNA, thiab cov proteins. Qhov kev sib cais ntawm biomolecules xws li DNA, RNA, thiab cov proteins los ntawm electrophoresis yog nyob ntawm tus nqi thiab qhov loj me. Cov qauv raug thauj mus rau hauv qhov dej ntawm cov gel. Tom qab ntawd, hluav taws xob teb yog siv thoob plaws gel. Lub teb ua rau tsis zoo-them molecules txav mus rau qhov zoo electrode. Lub gel matrix ua raws li cov molecular sieve los ntawm qhov uas cov molecules me tshaj plaws dhau los yog taug kev sai sai thaum cov molecules loj txav qeeb. Qhov no ua rau kev sib cais ntawm cov molecules raws li tus nqi thiab qhov loj me. Yog li, agarose thiab polyacrylamide gel electrophoresis yog ob hom gel electrophoresis cov txheej txheem uas feem ntau pab cais cov molecules raws li lawv qhov loj me thiab nqi.
Agarose Gel Electrophoresis yog dab tsi?
Agarose gel electrophoresis yog txheej txheem uas siv agarose gels cais tawm biomolecules xws li DNA thiab RNA. Nws yog cov txheej txheem siv los cais cov nucleic acids feem ntau los ntawm qhov loj me. Lub ntsiab lus tseem ceeb hu ua agarose siv hauv cov txheej txheem no yog polysaccharide. Nws los ntawm seaweeds. Agarose tuaj yeem yaj hauv qhov tsis muaj qhov kub thiab tom qab ntawd tuaj yeem nchuav rau hauv lub tais uas khaws cia kab rov tav. Nyob rau hauv lub tais, nws yuav solidified thaum nws txias los ua ib tug slab. Agarose gels tau nchuav nrog lub zuag hauv qhov chaw los ua qhov dej rau hauv cov nucleic acids xws li DNA lossis RNA tau thauj khoom thaum lub gel tau khov kho.
Daim duab 01: Agarose Gel Electrophoresis
Cov gel tom qab ntawd muab tso rau hauv qhov tsis zoo, thiab tam sim no tau siv thoob plaws gel. DNA muaj cov nqi tsis zoo uas tsis muaj kev ywj pheej ntawm cov kab ke ntawm cov molecule. Yog li, DNA molecules yuav tsiv los ntawm cathode (-) mus rau lub anode (+). Tus nqi ntawm kev tsiv teb tsaws yog ncaj qha nyob ntawm qhov loj ntawm cov molecule. Cov macromolecules loj tshaj plaws muaj lub sijhawm nyuaj tshaj plaws los ntawm cov gel. Ntawm qhov tod tes, cov macromolecules me me dhau los ntawm cov gel sai thiab yooj yim heev.
YPolyacrylamide Gel Electrophoresis yog dab tsi?
Polyacrylamide gel electrophoresis (PAGE) yog cov txheej txheem siv polyacrylamide gels cais tawm biomolecules. Nws yog cov txheej txheem uas tau siv dav los cais cov macromolecules lom, feem ntau yog cov proteins thiab nucleic acids, raws li lawv cov electrophoretic mobility. Lub hydration ntawm acrylonitrile ua rau tsim cov acrylamide molecules los ntawm nitrile hydratase. Acrylamide yog soluble hauv dej, thiab thaum ntxiv cov dawb radical pib, acrylamide polymerizes, uas ua rau tsim cov polyacrylamide gel. Feem ntau, kev nce ntxiv ntawm acrylamide ua rau txo qhov pore loj hauv cov gel. Polyacrylamide gels yog nchuav vertically, tsis zoo li agarose gels.
Daim duab 02: Polyacrylamide Gel Electrophoresis
Nyob hauv polyacrylamide gel electrophoresis, cov molecules yuav khiav hauv lawv lub xeev, khaws cia cov qauv kev txiav txim siab dua ntawm cov molecules. Txoj kev no hu ua haiv neeg PAGE. Xwb, cov tshuaj denaturant kuj tuaj yeem muab ntxiv kom tshem tawm cov qauv kev txiav txim siab dua thiab tig cov molecule mus rau hauv ib qho tsis muaj qauv molecule uas nws kev txav mus los tsuas yog nyob ntawm nws qhov ntev. Hom no hu ua SDS-PAGE.
Dab tsi yog qhov zoo sib xws ntawm Agarose thiab Polyacrylamide Gel Electrophoresis?
- Agarose thiab polyacrylamide gel electrophoresis yog ob hom gel electrophoresis cov txheej txheem.
- Qhov tseeb, lawv yog cov txheej txheem lom neeg molecular.
- Ob txoj kev siv los kuaj cov macromolecules lom xws li DNA thiab cov proteins.
- Cov tswv yim no yog ua los ntawm cov kws tshaj lij lossis cov kws tshawb fawb.
- Hauv ob txoj kev, kev sib cais ntawm macromolecules yog raws li tus nqi thiab qhov loj.
- Ob txoj kev tso cai rau kev sib cais ntawm nucleic acids zoo li DNA thiab RNA.
Qhov txawv ntawm Agarose thiab Polyacrylamide Gel Electrophoresis yog dab tsi?
Agarose gel electrophoresis yog ib qho txheej txheem uas siv cov kab rov tav nchuav agarose gels cais tawm biomolecules, thaum polyacrylamide gel electrophoresis yog cov txheej txheem uas siv vertically nchuav polyacrylamide gels cais tawm biomolecules. Qhov no yog qhov sib txawv tseem ceeb ntawm agarose thiab polyacrylamide gel electrophoresis. Tsis tas li ntawd, agarose gel electrophoresis yog siv rau kev sib cais ntawm DNA thiab RNA, thaum polyacrylamide gel electrophoresis yog siv rau kev sib cais ntawm nucleic acids xws li DNA, RNA, los yog proteins..
Cov lus hauv qab no qhia txog qhov sib txawv ntawm agarose thiab polyacrylamide gel electrophoresis.
Summary – Agarose vs Polyacrylamide Gel Electrophoresis
Agarose thiab polyacrylamide gel electrophoresis yog ob hom gel electrophoresis cov txheej txheem siv nyob rau hauv molecular biology kuaj. Agarose gel electrophoresis siv cov agarose gel cais tawm biomolecules. Agarose feem ntau tsis lom rau tib neeg, thaum polyacrylamide yog tshuaj lom rau tib neeg. Ntxiv mus, agarose gel electrophoresis qhia tau hais tias qhov kev daws teeb meem qis thaum polyacrylamide gel electrophoresis qhia tau hais tias muaj kev daws teeb meem ntau dua. Polyacrylamide gel electrophoresis siv polyacrylamide gel cais tawm biomolecules. Qhov no qhia txog qhov sib txawv ntawm agarose thiab polyacrylamide gel electrophoresis
