Qhov sib txawv tseem ceeb ntawm sonication thiab homogenization yog tias sonication yog cov txheej txheem cuam tshuam ntawm tes uas siv lub zog suab los cuam tshuam cov ntaub so ntswg thiab hlwb, thaum homogenization yog cov txheej txheem cuam tshuam ntawm tes uas feem ntau siv lub cev quab yuam kom tawg cell membranes.
txhawm rau txheeb xyuas biomolecules, lawv cov tshuaj tiv thaiv thiab lwm cov ntsiab lus ntawm tes, nws yuav tsum cuam tshuam cov ntaub so ntswg kom tso cov hlwb thiab cuam tshuam cov hlwb kom tso lawv cov ntsiab lus. Muaj cov txheej txheem lysis sib txawv. Sonication thiab homogenization yog ob txoj kev zoo li no. Sonication siv lub zog suab lossis ultrasonic tsis cuam tshuam rau cov hlwb thaum homogenization siv cov tshuab quab yuam los cuam tshuam cov hlwb.
Sonication yog dab tsi?
Sonication yog txoj kev cuam tshuam ntawm tes uas lub suab nrov nthwv dej ua rau lub hlwb. Hauv lwm lo lus, sonication siv lub zog ua kom tawg hlwb. Zoo ib yam li homogenization, sonication kuj yog ib qho kev cuam tshuam ntawm lub cev. Sonication yog qhov zoo tshaj plaws hauv kev cuam tshuam cov kab mob, cov poov xab, fungi, algae thiab mammalian hlwb. Thaum siv cov suab nrov nrov, nws ua kom muaj cua sov ntau. Li no, nws yog ib qho tsim nyog yuav tau ua sonication nyob rau hauv txias txias, tshwj xeeb tshaj yog immersing tus qauv nyob rau hauv ib tug dej khov da dej. Lub sonication yog qhov zoo tshaj plaws haum rau cov qauv uas muaj ntim hauv qab 100 mL.
Daim duab 01: Sonication
Sonicator yog cov cuab yeej siv hauv sonication. Piv rau lwm txoj kev, cell lysis los ntawm sonication yog ceev thiab yooj yim los tswj.
YHomogenization yog dab tsi?
Homogenization yog lwm txoj hauv kev cuam tshuam lub cev ntawm tes uas siv lub zog siv tshuab los ua kom cov hlwb tawg. Vim yog lub zog siv rau cov hlwb thiab cov ntaub so ntswg, cov cell membranes ntawm lub hlwb tawg. Mechanical homogenization, hlaws homogenization thiab sib tsoo yog ob peb hom homogenization hom.
Daim duab 02: Homogenization
Hauv kev sib xyaw tshuab homogenization, homogenizer yog siv. Nyob rau hauv cov hlaws homogenization, iav los yog hlau hlaws yog siv los thov ib tug maj mam abrasion. Hauv kev sib tsoo, siv lub cav thiab pestle. Ntxiv mus, hauv kev sib tsoo, khov cov qauv nrog kua nitrogen feem ntau ua tiav.
Dab tsi yog qhov zoo sib xws ntawm Sonication thiab Homogenization?
- Sonication thiab homogenization yog ob txoj hauv kev siv hauv kev cuam tshuam ntawm tes.
- Ob txoj hauv kev yog txoj hauv kev lub cev.
- Ntxiv mus, ob txoj hauv kev yog nrawm thiab yooj yim.
- Cov txheej txheem no zoo cuam tshuam cov kab mob, cov poov xab, fungi, algae thiab mammalian hlwb.
- Txawm li cas los xij, hauv ob txoj kev, protein denaturation thiab aggregation tuaj yeem tshwm sim.
- Ntxiv mus, kev tsim tawm tuaj yeem sib txawv ntawm ob txoj hauv kev.
Qhov txawv ntawm Sonication thiab Homogenization yog dab tsi?
Sonication yog ib txoj hauv kev lysis ntawm tes uas siv lub zog suab los cuam tshuam cov hlwb thaum homogenization yog ib txoj hauv kev siv lub cev ntawm cov cell lysis uas siv lub cev quab yuam kom tawg hlwb. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm sonication thiab homogenization. Dhau li ntawd, sonicator yog siv nyob rau hauv sonication, thaum homogenizer yog siv nyob rau hauv homogenization.
Ntxiv mus, sonication lyses hlwb sai heev, thaum homogenization yuav siv sij hawm ntau rau lyse hlwb dua sonication. Yog li ntawd, peb tuaj yeem xav txog qhov no kuj yog qhov sib txawv ntawm sonication thiab homogenization. Tsis tas li ntawd, qhov kev siv ntawm sonication yog yooj yim heev los kho, tso cai rau maj mam los yog sai sai cuam tshuam ntawm cell membranes, tsis zoo li homogenization.
Hauv qab infographic qhia txog qhov sib txawv ntawm sonication thiab homogenization.
Summary – Sonication vs Homogenization
Sonication thiab homogenization yog ob txoj kev cuam tshuam lub cev ntawm tes uas nquag siv hauv chav kuaj. Sonication siv ultrasound nthwv dej kom tawg hlwb thaum homogenization siv lub zog siv tshuab los cuam tshuam cov hlwb. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm sonication thiab homogenization. Sonication generates cua sov ntau dua li homogenization. Li no, nws yog ib qho tsim nyog yuav tau ua sonication nyob rau hauv txias txias. Ntxiv mus, sonication yog qhov zoo tshaj plaws haum rau cov qauv uas muaj tsawg dua 100 mL, tsis zoo li homogenization.
