Qhov sib txawv tseem ceeb ntawm chemiluminescence thiab electrochemiluminescence yog tias chemiluminescence yog emission ntawm hluav taws xob los yog lub teeb thaum lub sij hawm tshuaj tiv thaiv thaum electrochemiluminescence yog ib hom chemiluminescence uas tshwm sim los ntawm cov tshuaj tiv thaiv electrochemical tshwm sim hauv cov tshuaj.
Luminescence yog qhov emissions ntawm lub teeb lossis hluav taws xob los ntawm lub molecule lossis atom thaum nws los txog rau hauv av lub zog ntawm lub xeev zoo siab. Nws tsis yog qhov tshwm sim ntawm cua sov. Nws tuaj yeem tshwm sim thaum lub zog excitation absorbs lub zog ntawm lub teeb, tshuaj lom neeg cov tshuaj tiv thaiv los yog los ntawm cov tshuaj lom neeg catalyzed. Raws li qhov ntawd, luminescence tuaj yeem yog photoluminescence, chemiluminescence lossis bioluminescence. Chemiluminescence yog lub emission ntawm electromagnetic tawg xws li ultraviolet, pom, los yog infrared thaum lub sij hawm cov tshuaj tiv thaiv. Electroluminescence yog ib hom chemiluminescence. Nws tshwm sim vim muaj cov tshuaj tiv thaiv electrochemical tshwm sim hauv cov tshuaj.
Chemiluminescence yog dab tsi?
Chemiluminescence yog qhov emission ntawm lub teeb thaum muaj tshuaj tiv thaiv. Raws li qhov tshwm sim ntawm cov tshuaj tiv thaiv, ib qho ntawm cov khoom siv tshuaj tiv thaiv mus rau hauv lub xeev zoo siab thiab rov qab los rau hauv av hauv lub xeev ntawm lub zog los ntawm emitting hluav taws xob xws li ultraviolet, pom lossis infrared. Ib qho piv txwv ntawm chemiluminescence yog qhov ntsuas luminal. Hauv qhov kev sim no, ntshav qhia los ntawm luminescence vim kev sib cuag nrog hlau hauv hemoglobin.
Daim duab 01: Chemiluminescence
Feem ntau, cov tshuaj tiv thaiv tshuaj yuav tsum yog exothermic thiaj li tsim tau lub xeev zoo siab hauv hluav taws xob. Nyob rau hauv aqueous systems, chemiluminescence tshwm sim los ntawm redox cov tshuaj tiv thaiv. Muaj ntau ntau daim ntawv thov ntawm chemiluminescence. Hauv kev tshawb fawb forensic, chemiluminescence yog qhov tseem ceeb hauv kev daws teeb meem txhaum cai. Thaum txiav txim siab me me ntawm impurities lossis tshuaj lom hauv huab cua, tshuaj xyuas cov kab mob tsis muaj kab mob thiab cov kab mob organic hauv kev daws teeb meem, kuaj thiab tshuaj xyuas biomolecules thaum ELISA thiab Western blotting, sequencing DNA siv pyrosequencing, teeb pom kev zoo, teeb pom kev zoo cov khoom ua si, thiab lwm yam. chemiluminescence yog siv.
Electrochemiluminescence yog dab tsi?
Electrochemiluminescence yog qhov emission ntawm luminescence thaum muaj cov tshuaj tiv thaiv electrochemical. Nws yog ib hom chemiluminescence. Hauv lwm lo lus, electroluminescence yog chemiluminescence induced los ntawm electrochemical stimulus. Cov teeb liab luminescent no tshwm sim vim kev hloov pauv ntawm hluav taws xob hluav taws xob rau hauv hluav taws xob hluav taws xob los ntawm cov tshuaj tiv thaiv electrochemical. Excited-lub xeev cov khoom raug tsim los ntawm cov tshuaj tiv thaiv electrochemical.
Daim duab 02: Electrochemiluminescence
Electrochemiluminescence txheej txheem tsis xav tau cov cuab yeej kim. Kuj tseem muaj qhov me me ntawm kev cuam tshuam hauv cov txheej txheem no. Ntxiv mus, electrochemiluminescence yog nyob rau ntawm qhov chaw ntawm lub electrode los yog nws ze ze. Txawm hais tias qhov zoo no, nquag fouling ntawm cov electrodes yog ib qho tsis zoo ntawm cov txheej txheem no.
Feem ntau, electroluminescence tshwm sim tshwj xeeb tshaj yog thaum cov hlau chelates tau txais cov tshuaj lom neeg ntawm qhov chaw electrode. Muaj ntau ntau daim ntawv thov ntawm electrochemiluminescence. Thaum lub sij hawm DNA hybridization, cov ntawv cim electrochemiluminescent tshwj xeeb yog siv vim lawv muaj kev nkag siab zoo, yooj yim thiab ntau yam.
Dab tsi yog qhov zoo sib xws ntawm Chemiluminescence thiab Electrochemiluminescence?
- Electroluminescence yog ib hom chemiluminescence.
- Nyob rau hauv ob qho tib si, spontaneous emissions ntawm lub teeb tshwm sim.
- Muaj ntau daim ntawv thov ntawm electroluminescence thiab chemiluminescence.
- Ob qho tib si chemiluminescence thiab electroluminescence tshwm sim vim oxidation thiab txo cov tshuaj tiv thaiv.
Qhov txawv ntawm Chemiluminescence thiab Electrochemiluminescence yog dab tsi?
Chemiluminescence yog qhov emission ntawm lub teeb raws li qhov tshwm sim ntawm cov tshuaj tiv thaiv. Meanwhile, electrochemiluminescence yog lub emission ntawm lub teeb vim muaj cov tshuaj tiv thaiv electrochemical tshwm sim ntawm ib tug electrode nto. Yog li, qhov sib txawv tseem ceeb ntawm chemiluminescence thiab electrochemiluminescence yog tias chemiluminescence yog los ntawm cov tshuaj tiv thaiv tshuaj. Txawm li cas los xij, electrochemiluminescence yog qhov tshwm sim los ntawm kev txhawb nqa electrochemical. Ntxiv mus, electrochemiluminescence tshwm sim ntawm qhov chaw electrode thaum chemiluminescence tsis.
Cov ntawv qhia hauv qab no qhia cov ncauj lus kom ntxaws txog qhov sib txawv ntawm chemiluminescence thiab electrochemiluminescence.
Summary – Chemiluminescence vs Electrochemiluminescence
Chemiluminescence yog qhov emission ntawm lub teeb thaum muaj tshuaj tiv thaiv. Electrochemiluminescence yog ib hom luminescence uas tsim los ntawm cov tshuaj tiv thaiv electrode. Nws tseem hu ua electrogenerated chemiluminescence. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm chemiluminescence thiab electrochemiluminescence. Ob qhov xwm txheej tshwm sim vim yog cov khoom lag luam zoo siab poob rau hauv lub zog hauv av los ntawm lawv lub xeev zoo siab. Ntxiv mus, lawv muaj ntau daim ntawv thov nyob rau hauv txawv teb.
