Qhov sib txawv tseem ceeb ntawm chemiluminescence thiab fluorescence yog tias chemiluminescence yog lub teeb tawm los ntawm cov tshuaj tiv thaiv tshuaj, qhov fluorescence yog lub teeb tawm los ntawm kev nqus ntawm lub teeb lossis hluav taws xob hluav taws xob.
Chemiluminescence thiab fluorescence yog cov ntsiab lus tshuaj uas piav qhia lub teeb emission los ntawm ntau qhov chaw vim yog vim li cas sib txawv; e.g. tshuaj tiv thaiv los yog lub teeb haum. Lub teeb emitted yog lub npe hu ua luminescence, uas yog hais txog lub teeb spontaneous emission los ntawm qhov chaw.
Chemiluminescence yog dab tsi?
Chemiluminescence yog qhov emission ntawm lub teeb raws li qhov tshwm sim ntawm cov tshuaj tiv thaiv. Ntawm no, lub teeb emission hu ua luminescence. Qhov no txhais tau hais tias lub teeb emits raws li spontaneous emission, tsis yog los ntawm tshav kub los yog txias teeb. Txawm li cas los xij, cov cua sov kuj tuaj yeem tsim. Tom qab ntawd, cov tshuaj tiv thaiv ua rau exothermic.
Daim duab 01: Chemiluminescence
Thaum muaj tshuaj lom neeg, cov reactants sib tsoo nrog ib leeg, uas ua rau muaj kev cuam tshuam ntawm lawv. Tom qab ntawd, cov reactants sib xyaw ua ib qho kev hloov pauv. Cov khoom tsim los ntawm lub xeev kev hloov pauv no. Lub xeev hloov pauv muaj qhov siab tshaj plaws enthalpy / lub zog. Reactants thiab cov khoom muaj zog tsawg. Peb tuaj yeem muab lub npe hloov pauv raws li lub xeev zoo siab uas cov electrons zoo siab. Thaum cov hluav taws xob zoo siab rov qab los rau lub zog qub lossis lub xeev hauv av, lub zog ntau dhau raug tso tawm hauv daim ntawv ntawm photons. Lub teeb ntawm photons yog lub teeb uas peb tuaj yeem soj ntsuam thaum lub sijhawm Chemiluminescence.
Fluorescence yog dab tsi?
Fluorescence yog qhov emission ntawm lub teeb los ntawm cov khoom uas tau nqus lub zog yav dhau los. Cov tshuaj no yuav tsum nqus lub teeb lossis lwm yam hluav taws xob hluav taws xob kom tawm lub teeb ua fluorescence. Tsis tas li ntawd, lub teeb emitted no yog hom luminescence, txhais tau tias nws emits spontaneously. Lub teeb emitted feem ntau muaj lub wavelength ntev dua li lub teeb absorbed. Qhov ntawd txhais tau tias; lub teeb hluav taws xob emitted qis dua lub zog nqus.
Daim duab 02: Fluorescence ntawm Proteins
Thaum lub sijhawm txheej txheem ntawm fluorescence, lub teeb raug tawm los ntawm kev ua kom muaj zog ntawm atoms hauv cov khoom. Lub zog nqus tau feem ntau tso tawm raws li luminescence nyob rau lub sijhawm luv luv, txog 10 -8vib nas this. Qhov ntawd txhais tau tias; peb tuaj yeem soj ntsuam cov fluorescence sai li sai tau thaum peb tshem tawm cov hluav taws xob uas ua rau muaj kev zoo siab.
Muaj ntau daim ntawv thov ntawm fluorescence nyob rau hauv ntau qhov chaw, xws li mineralogy, gemology, tshuaj, tshuaj sensors, biochemical researches, dyes, bio detectors, fluorescent teeb tsim, thiab lwm yam. Ntxiv mus, peb tuaj yeem pom cov txheej txheem no raws li cov txheej txheem ntuj tsim thiab; Piv txwv li, nyob rau hauv ib co minerals.
Qhov txawv ntawm Chemiluminescence thiab Fluorescence yog dab tsi?
Chemiluminescence thiab fluorescence yog cov ntsiab lus tshuaj uas piav qhia lub teeb emission los ntawm ntau qhov chaw vim yog vim li cas sib txawv. Qhov sib txawv tseem ceeb ntawm chemiluminescence thiab fluorescence yog tias chemiluminescence yog lub teeb tawm los ntawm cov tshuaj tiv thaiv tshuaj, qhov fluorescence yog lub teeb tawm los ntawm kev nqus ntawm lub teeb lossis hluav taws xob hluav taws xob.
Ntxiv mus, hauv chemiluminescence, electrons ncav cuag lub xeev zoo siab vim qhov hloov pauv ntawm lub zog uas tshwm sim hauv cov tshuaj tiv thaiv tshuaj thaum nws nce los ntawm cov tshuaj reactants rau cov khoom. Tab sis, nyob rau hauv fluorescence, cov electrons mus txog lub xeev zoo siab vim lub zog absorbed los ntawm ib tug electromagnetic qhov chaw. Tsis tas li ntawd, peb tuaj yeem soj ntsuam lub teeb tawm tom qab ua tiav cov tshuaj tiv thaiv hauv chemiluminescence. Lub caij no, nyob rau hauv fluorescence, peb tuaj yeem soj ntsuam luminescence sai sai tom qab tshem tawm ntawm qhov chaw ntawm hluav taws xob hluav taws xob.
Hauv qab infographic tabulates qhov sib txawv ntawm chemiluminescence thiab fluorescence.
Summary – Chemiluminescence vs Fluorescence
Chemiluminescence thiab fluorescence yog cov ntsiab lus tshuaj uas piav qhia lub teeb emission los ntawm ntau qhov chaw vim yog vim li cas sib txawv. Qhov sib txawv tseem ceeb ntawm chemiluminescence thiab fluorescence yog tias chemiluminescence yog lub teeb tawm los ntawm cov tshuaj tiv thaiv tshuaj, qhov fluorescence yog lub teeb tawm los ntawm kev nqus ntawm lub teeb lossis hluav taws xob hluav taws xob.