Qhov Tseem Ceeb Qhov txawv - Gram Stain vs Culture
Gram stain yog txheej txheem staining uas tau ua los sib txawv cov kab mob ua ob pawg raws li cov tuab ntawm peptidoglycan txheej hauv lawv cov phab ntsa ntawm tes. Kab lis kev cai yog ib txoj hauv kev loj hlob thiab tswj cov kab mob nyob rau hauv lub chaw kuaj mob rau kev soj ntsuam sib txawv. Qhov sib txawv tseem ceeb ntawm grams stain thiab kab lis kev cai yog lawv txoj haujlwm; gram strain cov txheej txheem staining ntawm cov kab mob uas kab lis kev cai yog ib txoj hauv kev loj hlob thiab tswj cov kab mob.
Gram Stain yog dab tsi?
Gram stain yog ib qho tseem ceeb sib txawv staining txheej txheem siv rau kev txheeb xyuas kab mob hauv Microbiology. Cov txheej txheem no tau qhia los ntawm Danish Bacteriologist Hans Christian Gram hauv xyoo 1884. Gram staining categorizes cov kab mob ua ob pawg loj: gram positive thiab gram negative; cov no yog ib qho tseem ceeb heev nyob rau hauv cov kab mob kev faib thiab kev txheeb xyuas. Gram staining yog ua raws li thawj kauj ruam rau cov kab mob kab mob.
Cov kab mob yog pawg raws li qhov sib txawv ntawm lawv cov phab ntsa ntawm tes. Cov kab mob Gram zoo muaj cov txheej peptidoglycan tuab nyob rau hauv lawv cov phab ntsa ntawm tes thaum Gram tsis zoo cov kab mob muaj ib txheej nyias peptidoglycan nyob rau hauv lawv cov phab ntsa cell raws li qhia nyob rau hauv daim duab 01. Qhov tshwm sim ntawm gram staining yuav ua raws li cov thickness sib txawv nyob rau hauv peptidoglycan txheej. cell phab ntsa.
Daim duab 1: Cov kab mob Gram positive thiab Gram negative bacteria
Grams staining yog ua los ntawm plaub yam reagents xws li; thawj stain, mordant, decolorizing tus neeg saib xyuas thiab txee stain. Crystal violet thiab safranin ua haujlwm tseem ceeb thiab cov stains, ntsig txog thaum cov grams iodine thiab 95% cawv ua cov mordant thiab decolourizer, feem.
Basic Steps of Grams Stain
- Ib kab mob smear yog npaj rau ntawm lub khob huv si, kho cua sov thiab txias.
- Smear yog dej nyab nrog siv lead ua violet rau 1 - 2 feeb.
- Smear yog yaug nrog dej ntws qeeb kom tshem tawm cov stains ntau dhau.
- Grams iodine yog siv rau smear rau 1 feeb.
- Smear yaug nrog dej ntws qeeb
- Smear yog ntxuav nrog 95% cawv rau 2 - 5 vib nas this thiab yaug nrog dej ntws qeeb.
- Smear yog counter stained nrog safranin rau 1 feeb
- Smear yog yaug nrog dej ntws qeeb, qhuav thiab pom nyob rau hauv lub tshuab ntsuas.
Thaum kawg ntawm cov kab mob gram tsis zoo, cov kab mob gram tsis zoo yuav raug pom hauv cov xim liab thaum cov kab mob gram zoo yuav pom cov xim liab raws li pom hauv daim duab 02. Qhov tshwm sim ntawm cov kab mob gram tsis zoo yog txiav txim siab los ntawm cov tuab. ntawm peptidoglycan txheej hauv lawv cov phab ntsa ntawm tes. Thaum lub sij hawm decolorizing kauj ruam, thawj stain thiab mordant tau yooj yim tshem tawm ntawm cov kab mob gram tsis zoo thiab ua tsis muaj xim vim lawv muaj txheej nyias peptidoglycan. Thawj qhov stain yog khaws cia rau hauv cov kab mob zoo vim tias lawv muaj txheej peptidoglycan tuab. Lub txee stain yuav tsis zoo rau cov kab mob uas muaj kab mob zoo vim yog khaws cia ntawm cov kab mob thawj zaug. Yog li ntawd, cov kab mob zoo yuav pom nyob rau hauv thawj xim xim, piv txwv li, xim liab. Lub txee stain yuav stain cov kab mob gram tsis zoo thiab yuav pom hauv cov xim xaiv uas yog xim safranin. Yog li ntawd, nws yog ib qho yooj yim rau categorize cov kab mob rau hauv ob pawg los ntawm gram stain thiab nws yog ib qho tseem ceeb nyob rau hauv cov kab mob sib txawv thiab kev txheeb xyuas.
Daim duab 2: Gram Strain
Kab lis kev cai yog dab tsi?
kab lis kev cai kab lis kev cai kab lis kev cai yog ib txoj hauv kev ntawm kev coj noj coj ua thiab tswj cov kab mob hauv cov chaw kuaj mob rau ntau lub hom phiaj. Kab lis kev cai yog zus nyob rau hauv cov khoom, semi-kua thiab kua xov xwm raws li hom thiab lub hom phiaj ntawm microorganism culturing. Kab lis kev cai yog muab nrog cov as-ham tsim nyog thiab kev loj hlob tej yam kev mob xav tau los ntawm cov kab mob. Muaj cov khoom sib txawv ntawm cov kab lis kev cai xws li lub zog qhov chaw, carbon qhov chaw, nitrogen qhov chaw, cov zaub mov, micronutrients, dej, solidifying agent, thiab lwm yam. Qhov zoo tshaj plaws kub, oxygen thiab pH yuav tsum tau kho raws li hom microorganisms zus.
Muaj ntau hom kab mob microbial; Piv txwv li, kab lis kev cai batch, kab lis kev cai txuas ntxiv, kab lis kev cai stab, kab lis kev cai agar phaj, kab lis kev cai broth, thiab lwm yam. Raws li qhov muaj pes tsawg leeg ntawm qhov loj hlob nruab nrab, muaj ntau hom kab lis kev cai xov xwm hu ua hluavtaws media, semi-synthetic media thiab natural media. Cov kab lis kev cai microbial tau npaj nyob rau hauv cov xwm txheej tsis muaj menyuam nyob hauv ib chav tshwj xeeb hu ua laminar air flow. Loj hlob nruab nrab thiab cov khob iav yog sterilized ua ntej inoculation ntawm cov kab mob uas xav tau. Nyob rau hauv cov xwm txheej kom tsis muaj menyuam, lub hom phiaj kab mob kis mus rau hauv qhov nruab nrab uas tsis muaj zaub mov thiab incubated ntawm qhov kub zoo. Nyob rau hauv nruab nrab, cov kab mob yuav loj hlob thiab siv cov as-ham.
Daim duab 3: Kab lis kev cai kab mob ntawm phaj
Dab tsi yog qhov txawv ntawm Gram Stain thiab Culture?
Gram Stain vs Culture |
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| Grams strain yog cov txheej txheem staining sib txawv siv rau cov kab mob sib txawv thiab kev txheeb xyuas. | kab lis kev cai kab mob microbial yog ib txoj hauv kev loj hlob kab mob hauv chav kuaj. |
| Qhov no siv cov tshuaj sib txawv nrog rau ob lub stains. | Qhov no siv cov kab lis kev cai sib txawv xws li cov khoom siv, cov khoom ib nrab thiab cov kua ua kua uas muaj cov as-ham thiab lwm yam tsim nyog. |
| Cov Haujlwm Tseem Ceeb | |
| Qhov no tso cai rau kev faib cov kab mob ua ob pawg: grams tsis zoo thiab grams zoo. | Qhov no tso cai rau kev sib npaug ntawm cov kab mob sib txawv rau lub hom phiaj sib txawv. |
| Base | |
| Gram stain tshwm sim yog raws li qhov sib txawv ntawm peptidoglycan txheej ntawm phab ntsa cell. | Cov kab mob yuav loj hlob thiab sib npaug hauv kab lis kev cai xov xwm. |
| Cov kab mob tsis zoo pom muaj xim liab thiab cov kab mob zoo pom muaj xim liab. | Ntawm daim phiaj, cov kab mob ntawm cov kab mob tuaj yeem pom. Ntawm cov kua dej, cov kab mob muaj nyob hauv daim ntawv raug tshem tawm. |
Summary – Gram Stain vs Culture
kab lis kev cai kab mob microbial tau npaj thiab khaws cia nyob rau hauv lub chaw kuaj mob rau ntau lub hom phiaj xws li khaws cia, kuaj, tshuaj purification, thiab lwm yam. Grams stain yog txheej txheem staining uas sib txawv cov kab mob rau hauv ob pawg tseem ceeb hu ua grams negative bacteria thiab grams positive bacteria. Yog li, qhov sib txawv ntawm cov grams stain thiab kab lis kev cai yog tias grams stain yog cov txheej txheem staining ntawm cov kab mob thaum kab lis kev cai yog ib txoj hauv kev loj hlob thiab tswj cov kab mob hauv cov chaw sim.
