Qhov Tseem Ceeb Qhov txawv - Capillary Electrophoresis vs Gel Electrophoresis
Electrophoresis yog ib qho txheej txheem uas siv los cais cov biomolecules raws li tus nqi particle, qhov loj me, thiab cov duab particle. Kev tsiv teb tsaws ntawm cov molecule, hu ua electrophoretic mobility, nyob ntawm seb hom polymer / gel siv, nws qhov pore loj, qhov hluav taws xob muab, lub sij hawm khiav thiab qhov chaw rau ntim piv. Muaj ntau hom kev siv electrophoresis raws li hom biomolecule siv. Thawj hom electrophoresis tau tsim yog daim ntawv electrophoresis qhov twg daim ntawv nitrocellulose tau siv los ua qhov nruab nrab rau kev sib cais ntawm biomolecules. Lub hauv paus ntsiab lus ntawm gel electrophoresis qhov sib txawv pore loj gels tau siv los cais biomolecules, tau tsim tom qab. Gel electrophoresis cov txheej txheem tau hloov kho ntxiv los txhim kho qhov tseeb ntawm cov txheej txheem, thiab ib qho kev hloov kho yog capillary electrophoresis. Qhov sib txawv tseem ceeb ntawm capillary electrophoresis thiab gel electrophoresis yog tias gel electrophoresis tau ua nyob rau hauv ntsug lossis kab rov tav dav hlau siv cov polymer gel ntawm tus qauv pore loj whereas capillary electrophoresis yog ua nyob rau hauv capillary raj nrog ib tug polymer kua los yog ib tug gel.
YGel Electrophoresis yog dab tsi?
Gel electrophoresis yog txheej txheem siv los cais cov nucleic acids, proteins lossis amino acids raws li nws cov nqi, qhov loj me, thiab cov duab. Cov txheej txheem no siv lub cev gel, uas yog cov khoom siv polymer, ua qhov nruab nrab ntawm kev sib cais. Feem ntau siv cov gels yog Agarose (rau nucleic acid sib cais) thiab Polyacrylamide (rau protein sib cais). Lub gel electrophoretic apparatus muaj cov gel casting tais los npaj cov gel, casting combs los npaj lub qhov dej, tsis tank, electrodes - zoo (anode) thiab tsis zoo (cathode) thiab lub voltage mov unit. Cov molecules zoo li DNA lossis RNA, uas raug them tsis zoo, txav los ntawm cathode mus rau anode thiab cov molecules uas raug them zoo txav mus rau lwm qhov. Kev npaj gel yog ua tiav raws li qhov yuav tsum tau ua. Yog tias xav tau kev daws teeb meem siab lossis kev sib cais ntawm cov molecules, lub siab concentration gel nrog lub pore me me yuav tsum tau npaj. Cov molecules sib cais ntawm cov gel matrix tau pom tom qab cov txheej txheem staining. Cov molecules sib cais tshwm sim raws li pawg ntawm cov gel matrix.
Daim duab 01: Gel Electrophoresis
Gel electrophoresis yog siv rau hauv kev kuaj mob molecular xws li DNA ntiv tes los txiav txim siab muaj qee yam DNA / RNA tawg lossis cov protein. Gel electrophoresis kuj txiav txim siab qhov purity ntawm cov qauv biomolecule rho tawm. Gel electrophoresis yog ua raws li cov kauj ruam ua ntej rau hauv thiab hybridization thiab raws li kev soj ntsuam xyuas tom qab sequencing.
Capillary Electrophoresis yog dab tsi?
Capillary electrophoresis yog ib qho kev hloov kho ntawm gel electrophoresis uas siv tib lub hauv paus ntsiab lus ntawm kev sib cais raws li tus nqi, qhov loj ntawm cov molecule, tab sis yog ua nyob rau hauv ib tug capillary raj nrog ib tug gel tshuaj los yog kua polymer. Capillaries yog npaj los ntawm fused silica, thiab txhua lub raj capillary muaj ib txoj kab uas hla ntawm 50-100μm thiab yog 25-100cm nyob rau hauv ntev. Cov qauv raug txhaj rau hauv lub raj capillary uas muaj cov khoom siv polymer thiab sib cais sai dua li cov pa gel electrophoresis. Lub kaw lus capillary muaj kev tiv thaiv zoo hauv lub tsho insulator uas tiv thaiv cov qauv los ntawm kev sib kis. Capillaries tuaj yeem sau nrog cov kua polymers xws li hydroxyethyl cellulose lossis cov gels siab xws li polyacrylamide. Capillary electrophoresis muab kev daws teeb meem ntau dua; yog li kev sib cais yog qhov tseeb dua. Capillary electrophoresis siv lub tshuab ntsuas hluav taws xob los ntawm kev tsom xam spectrophotometric. Qhov no yog vim qhov chaw siab dua rau qhov ntim piv.
Daim duab 02: Capillary Electrophoresis
Capillary electrophoresis yog siv rau hauv cov xwm txheej xws li hauv kev tshawb fawb uas yuav tsum tau muaj qhov raug siab dua thiab tsis siv ntau vim nws yog cov txheej txheem kim.
Dab tsi yog qhov zoo sib xws ntawm Capillary Electrophoresis thiab Gel Electrophoresis?
- Kev sib cais ntawm cov molecules hauv ob txoj kev yog ua raws li tus nqi thiab qhov loj ntawm cov molecule.
- Ob txoj kev siv tau los cais cov nucleic acids thiab proteins.
- Tus qauv ntim ntawm ob txoj kev yog tib yam.
- Ob txoj kev siv cov khoom siv los pab kom sib cais.
Qhov txawv ntawm Capillary Electrophoresis thiab Gel Electrophoresis yog dab tsi?
Capillary Electrophoresis vs Gel Electrophoresis |
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| Capillary electrophoresis yog ib qho txheej txheem uas cais cov biomolecules ntawm lub raj capillary siv cov kua lossis gel polymer nruab nrab. | Gel electrophoresis yog ib qho txheej txheem uas cais cov biomolecules ntawm lub dav hlau ntsug lossis kab rov tav siv lub nruab nrab polymer gel. |
| Kev sib cais | |
| Nyob rau hauv capillary electrophoresis, kev sib cais yog ua tiav hauv lub raj capillary. | Hauv gel electrophoresis, kev sib cais yog ua tiav ntawm lub dav hlau ntsug lossis kab rov tav. |
| Nruab nrab ntawm kev sib cais | |
| Cov kua polymers zoo li hydroxyethylcellulose yog siv hauv capillary electrophoresis. | Gels, xws li agarose lossis polyacrylamide, yog siv los ua qhov nruab nrab hauv gel electrophoresis. |
| Cross Linkage | |
| Kev daws teeb meem siab tuaj yeem tau txais los ntawm capillary electrophoresis. | Kev daws teeb meem tsawg hauv gel electrophoresis. |
| Ntoj thaj tsam rau Volume Ratio | |
| Nto rau ntim piv yog siab hauv capillary electrophoresis. | Ntoog rau ntim piv yog tsawg hauv gel electrophoresis. |
| Kev Tshawb Fawb | |
| Kev kuaj pom yog ua tiav los ntawm spectrophotometric automated detectors hauv capillary electrophoresis. | Staining thiab soj ntsuam los ntawm UV transilluminator yog ua raws li kev tshawb nrhiav hauv gel electrophoresis. |
Summary – Capillary Electrophoresis vs Gel Electrophoresis
Molecular diagnostics plays lub luag haujlwm tseem ceeb hauv ntiaj teb kev tshawb fawb. Kev txheeb xyuas thiab ua kom huv ntawm DNA, RNA, thiab Proteins yog cov kauj ruam tseem ceeb hauv cov txheej txheem kuaj mob. Electrophoresis yog ib qho txheej txheem uas cais thiab txheeb xyuas cov biomolecules hauv ob qho tib si gel electrophoresis thiab ntau cov capillary gel electrophoresis. Gel electrophoresis yog ua nyob rau hauv ib tug ntsug los yog kab rov tav dav hlau siv ib tug polymer gel ntawm standard pore loj whereas capillary electrophoresis yog ua nyob rau hauv ib tug capillary raj nrog ib tug polymer kua los yog ib tug gel. Qhov no yog qhov txawv ntawm capillary electrophoresis thiab gel electrophoresis. Tom qab ua tiav cov txheej txheem electrophoresis, biomolecules tau ua tiav ntxiv kom tau txais cov ntaub ntawv qib siab dua ntawm kev sib xyaw los yog los ntawm cov txheej txheem xws li kev luam ntiv tes.
Download PDF Version of Capillary Electrophoresis vs Gel Electrophoresis
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Gel Electrophoresis thiab Capillary Electrophoresis.
