Qhov Tseem Ceeb Qhov txawv - Kab rov tav vs Vertical Gel Electrophoresis
Gel electrophoresis yog cov txheej txheem kuaj siv hauv cov noob caj noob ces cais cov khoom sib xyaw uas muaj DNA, RNA, thiab lwm cov proteins raws li lawv cov nqi thiab cov molecular loj. DNA, RNA los yog proteins uas yuav tsum tau muab cais nyob rau hauv txoj kev no yog khiav los ntawm ib tug gel uas muaj me me pores. Cov molecules yog tsav los ntawm gel los ntawm ib qho hluav taws xob teb. Cov molecules dhau los ntawm qhov pores ntawm cov gel, thiab qhov ceev ntawm lub zog yog inversely proportional rau lawv qhov ntev. Yog li ntawd, cov molecules nrog qis molecular loj yuav txav nrawm dua li cov molecules uas muaj qhov hnyav dua molecular. Lub teb hluav taws xob yog tsim los ntawm qhov sib txawv ntawm tus nqi ntawm ob qhov kawg ntawm gel. Ib kawg muaj tus nqi zoo, thiab lwm qhov kawg muaj tus nqi tsis zoo. Txij li thaum DNA thiab RNA cov molecules raug them tsis zoo, lawv yuav raug nyiam mus rau qhov kawg ntawm cov gel. Gel electrophoresis tuaj yeem yog ob txoj kev sib txawv: kab rov tav gel electrophoresis thiab ntsug gel electrophoresis. Nyob rau hauv kab rov tav gel electrophoresis, cov gel yog tam sim no nyob rau hauv ib tug kab rov tav orientation thiab yog submerged nyob rau hauv ib tug nruam khiav tsis uas yog tam sim no nyob rau hauv lub gel lub thawv nws tus kheej. Nyob rau hauv ntsug gel electrophoresis, lub tshuab tsis yog oriented vertically thiab yog discontinuous nrog ob chav nyob rau sab saum toj thiab hauv qab nrog ib tug cathode thiab ib tug anode, feem. Qhov no yog qhov sib txawv tseem ceeb ntawm kab rov tav thiab ntsug gel electrophoresis.
Kab rov tav Gel Electrophoresis yog dab tsi?
Kab rov tav gel electrophoresis siv cov kev xav hauv paus rau kev sib cais ntawm DNA, RNA lossis cov protein molecules raws li lawv qhov loj me me thiab nqi. Nyob rau hauv cov txheej txheem no, cov gel yog tam sim no nyob rau hauv ib tug kab rov tav orientation thiab yog submerged nyob rau hauv ib tug tsis tu ncua. Agarose gel yog siv los cais lub gel lub thawv rau hauv ob lub thawv. Ib qho kawg ntawm lub thawv gel muaj ib qho anode thaum lwm qhov kawg muaj cathode. Thaum siv tam sim no, qhov tsis siv rau hauv cov txheej txheem no tso cai rau kev tsim cov nqi gradient. Thaum tus nqi raug thov, cov gel zoo li kub. Lub tsis tuaj yeem ua haujlwm raws li lub tshuab ua kom txias, uas tswj qhov kub ntawm qhov zoo tshaj plaws. Recirculation ntawm cov khiav tsis txwv tsis pub tsim pH gradient. Lub kaw lus tsis tu ncua tsis tuaj yeem siv rau hauv kab rov tav gel electrophoresis txij li ob qhov chaw ntawm lub gel system tau txuas nrog cov tsis ua haujlwm. Acrylamide yog siv thaum lub sij hawm gel electrophoresis cais cov protein sib tov.
Daim duab 01: Kab rov tav Gel Electrophoresis
Hauv kab rov tav gel electrophoresis, acrylamide tsis tuaj yeem siv vim tias lub thawv gel raug rau oxygen. Vim muaj cov pa oxygen, polymerization ntawm acrylamide inhibited, thiab qhov no cuam tshuam nrog kev tsim cov gel. Kab rov tav gel electrophoresis yog ib txoj hauv kev siv zog sib cais ntawm DNA thiab RNA.
Vertical Gel Electrophoresis yog dab tsi?
Vertical gel electrophoresis cov txheej txheem ua haujlwm raws li thawj txoj kev xav ntawm gel electrophoresis, tab sis nws tau suav tias yog qhov nyuaj dua li kab rov tav gel electrophoresis txoj kev. Cov txheej txheem no siv lub tshuab tsis txuas ntxiv. Ib tug cathode nyob rau hauv lub sab saum toj chamber, thiab lub anode nyob rau hauv qab chamber. Cov electrodes tam sim no nyob rau hauv txhua qhov chaw muab cov hluav taws xob xav tau. Ib txheej nyias ntawm gel yog nchuav ntawm ob daim iav mounted. Yog li ntawd, sab saum toj ntawm cov gel yog submerged nyob rau hauv lub sab saum toj chamber, thiab cov nram qab no feem ntawm cov gel yog submerged nyob rau hauv lub chamber nyob rau hauv qab. Thaum siv tam sim no, ib feem me me ntawm qhov tsis txav mus rau hauv qab chamber los ntawm lub chamber sab saum toj los ntawm cov gel. Tam sim no siv nyob rau hauv cov txheej txheem no yog ib feeb units.
Daim duab 02: Vertical Gel Electrophoresis
Nyob rau hauv ntsug gel electrophoresis, tsis tsuas ntws los ntawm cov gel. Qhov no tso cai rau kev tswj kom raug ntawm gradient ntawm voltage thaum lub sij hawm sib cais. Acrylamide gel tuaj yeem siv tau vim tias cov khoom ntim tsis raug rau cov pa oxygen. Vim qhov me me pore loj ntawm acrylamide gel, kev sib cais meej tuaj yeem ua tiav nrog kev daws teeb meem siab dua.
Dab tsi yog qhov zoo sib xws ntawm Kab rov tav thiab ntsug Gel Electrophoresis?
- Ob lub tshuab ua haujlwm raws li kev xav ntawm gel electrophoresis.
- Anode thiab cathode yog siv los muab cov hluav taws xob xav tau hauv ob lub tshuab.
Dab tsi yog qhov txawv ntawm Kab rov tav thiab ntsug Gel Electrophoresis?
Kab rov tav vs Vertical Gel Electrophoresis |
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| Kab rov tav Gel Electrophoresis yog cov txheej txheem gel electrophoresis uas cov gel tam sim no nyob rau hauv kab rov tav. | Vertical Gel Electrophoresis yog cov txheej txheem gel electrophoresis uas cov gel yog taw qhia vertically. |
| Kab rov tav gel electrophoresis muaj qhov tsis tu ncua. | Kev khiav tsis tu ncua tsis tu ncua hauv ntsug gel electrophoresis. |
| Siv Acrylamide | |
| Acrylamide tsis tuaj yeem siv rau kab rov tav gel electrophoresis txij li lub thawv gel raug rau huab cua oxygen. | Vim tias cov gel tsis raug rau cov pa oxygen vim yog ob chav sib cais, acrylamide tuaj yeem siv rau ntsug gel electrophoresis. |
| Kab rov tav gel electrophoresis feem ntau siv rau kev sib cais ntawm DNA thiab RNA sib xyaw tab sis tsis yog cov protein. | Vertical gel electrophoresis yog siv los cais cov protein sib xyaw. |
Summary – Kab rov tav vs Vertical Gel Electrophoresis
Gel electrophoresis yog cov txheej txheem kuaj uas tau siv dav hauv kev sib cais ntawm cov khoom sib xyaw uas muaj cov molecules ntawm DNA, RNA, thiab cov proteins. Muaj ob txoj hauv kev ntawm gel electrophoresis: kab rov tav thiab ntsug gel electrophoresis. Nyob rau hauv kab rov tav gel electrophoresis, lub khiav tsis yog tas li thaum nyob rau hauv ntsug gel electrophoresis, nws yog discontinuous. Qhov no yog qhov txawv ntawm kab rov tav thiab ntsug gel electrophoresis. Ob lub tshuab ua haujlwm raws li lub hauv paus ntsiab lus ntawm gel electrophoresis.
Download PDF Version of Kab rov tav vs Vertical Gel Electrophoresis
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Kab rov tav thiab ntsug Gel Electrophoresis.
