Qhov sib txawv tseem ceeb ntawm gel thiab ntawv electrophoresis yog qhov nruab nrab ntawm kev sib cais hauv gel electrophoresis yog agarose gel, qhov nruab nrab ntawm kev sib cais hauv ntawv electrophoresis yog daim ntawv strip rau hauv cov tshuaj tsis haum.
Electrophoresis yog cov txheej txheem kev tshuaj xyuas uas muaj txiaj ntsig zoo rau kev tshuaj xyuas tus qauv siv cov khoom hluav taws xob ntawm cov tshuaj muaj nyob hauv cov qauv ntawd. Ntawm no, peb tuaj yeem soj ntsuam cov lus tsa suab ntawm dispersed solute nyob rau hauv nruab nrab tsom xam. Yog li ntawd, peb tuaj yeem txiav txim siab qhov kev txav ntawm cov tshuaj lom neeg txheeb ze rau nruab nrab. Gel electrophoresis thiab ntawv electrophoresis yog ob yam tseem ceeb hauv chemistry.
YGel Electrophoresis yog dab tsi?
Gel electrophoresis tuaj yeem piav qhia raws li kev sib cais thiab kev tshuaj xyuas ntawm macromolecules nyob ntawm qhov loj thiab nqi. Macromolecules hauv cov ntsiab lus no tuaj yeem xa mus rau DNA, RNA, cov proteins, thiab lawv cov khoom tawg. Txoj kev no muaj txiaj ntsig zoo hauv kev kho mob chemistry rau kev sib cais ntawm cov protein los ntawm kev them nqi lossis loj. Tsis tas li ntawd, nws yog ib qho tseem ceeb hauv biochemistry thiab molecular biology rau kev sib cais ntawm cov pej xeem sib xyaw ntawm DNA thiab RNA fragments los ntawm lawv qhov ntev los kwv yees qhov loj ntawm DNA thiab RNA fragments. Dhau li ntawd, peb tuaj yeem siv nws cais cov proteins los ntawm lawv cov nqi.
Daim duab 01: Gel Electrophoresis Instrumentation
Peb tuaj yeem cais cov nucleic acid molecules los ntawm kev siv hluav taws xob rau kev txav ntawm cov khoom tsis zoo los ntawm cov matrix ntawm agarose lossis lwm yam tshuaj. Hauv cov txheej txheem no, luv luv molecules tuaj yeem txav tau sai dua thaum cov molecules ntev txav qeeb. Qhov no yog vim luv luv molecules tuaj yeem txav los ntawm cov gel pores yooj yim. Peb hu qhov kev txav ntawm qhov tawg ntawm qhov pores "sieving." Ntxiv mus, peb feem ntau tsis tuaj yeem cais cov proteins raws li lawv qhov loj me los ntawm txoj kev no vim tias cov protein ntau dhau los ua sieved los ntawm gel pores. Txawm li cas los xij, peb tuaj yeem siv cov txheej txheem no los cais cov nanoparticles.
Daim Ntawv Electrophoresis yog dab tsi?
Daim ntawv electrophoresis tuaj yeem piav qhia raws li kev sib cais siv cov ntawv lim dej uas tau ntim rau hauv cov tshuaj tsis haum. Feem ntau, peb siv diethylbarbituric acid thiab barbituric acid yaj hauv alkali ua cov tshuaj tsis haum. Tus nqi pH ntawm cov tshuaj tsis haum no yog pH 8.6. Tsis tas li ntawd, peb tuaj yeem tso me me ntawm cov kua dej rau ntawm daim ntawv, thiab qhov ncaj qha tam sim no dhau los ntawm nws rau ob peb teev.
Daim ntawv electrophoresis yog ib qho tseem ceeb rau kev sib cais ntawm cov me me, them cov molecules, suav nrog cov amino acids thiab cov proteins me. Ntawm no, peb yuav tsum tau moisten ib daim kab xev ntawm cov ntawv lim nrog tsis thiab raus qhov kawg ntawm lub sawb mus rau hauv tsis reservoirs uas muaj electrodes. Thawj tus neeg tshaj tawm uas siv txoj kev no yog Konig hauv xyoo 1937. Hauv nws qhov kev tshawb pom, nws tau qhia ib daim ntawv-soaked tsis rau cheeb tsam electrophoresis thiab kuj qhia txog kev kuaj UV.
Qhov txawv ntawm Gel thiab Ntawv Electrophoresis yog dab tsi?
Electrophoresis yog cov txheej txheem tshuaj xyuas uas muaj txiaj ntsig zoo rau kev tshuaj xyuas cov qauv siv hluav taws xob ntawm cov tshuaj lom neeg muaj nyob hauv cov qauv ntawd. Gel electrophoresis thiab ntawv electrophoresis yog ob qho tseem ceeb electrophoresis txoj kev. Qhov sib txawv tseem ceeb ntawm gel thiab ntawv electrophoresis yog qhov nruab nrab ntawm kev sib cais hauv gel electrophoresis yog agarose gel, whereas qhov nruab nrab ntawm kev sib cais hauv ntawv electrophoresis yog daim ntawv strip rau hauv cov tshuaj tsis haum.
Hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm gel thiab ntawv electrophoresis hauv daim ntawv tabular rau sab sib piv.
Summary – Gel vs Paper Electrophoresis
Gel electrophoresis yog txoj kev sib cais thiab kev tshuaj xyuas ntawm macromolecules nyob ntawm qhov loj thiab nqi, thaum daim ntawv electrophoresis yog kev sib cais siv cov ntawv lim dej so hauv cov tshuaj tsis haum. Qhov sib txawv tseem ceeb ntawm gel thiab ntawv electrophoresis yog qhov nruab nrab ntawm kev sib cais hauv gel electrophoresis yog agarose gel, qhov nruab nrab ntawm kev sib cais hauv ntawv electrophoresis yog daim ntawv strip rau hauv cov tshuaj tsis haum.
