Qhov sib txawv tseem ceeb ntawm 1D thiab 2D gel electrophoresis yog cov khoom siv rau kev sib cais ntawm cov protein ntawm gel electrophoresis. 1D gel electrophoresis tsuas yog cais cov proteins raws li qhov hnyav molecular thaum 2D gel electrophoresis cais cov proteins raws li nws cov iso-hluav taws xob taw tes thiab qhov hnyav molecular.
Kev sib cais ntawm cov protein los ntawm gel electrophoresis yog cov txheej txheem tseem ceeb los qhia cov proteins. Proteins muaj cov khoom sib txawv; Yog li ntawd, kev sib cais yog qhov nyuaj dua piv rau DNA sib cais los ntawm Agarose gel electrophoresis.
1D Gel Electrophoresis yog dab tsi?
1D Gel Electrophoresis, tseem hu ua ib qhov ntev gel electrophoresis, yog ib txoj kev sib cais ntawm cov protein raws li qhov hnyav molecular. Kev sib cais ntawm cov protein feem ntau yog siv los ntawm polyacrylamide gel electrophoresis. Raws li lub tswv yim ntawm gel electrophoresis, molecules cais ntawm lawv cov khoom ntawm molecular hnyav thiab nqi.
Yog li ntawd, txhawm rau muab cov nqi sib xws rau cov protein, Sodium dodecyl sulfate (SDS) kev kho mob ua ntej gel electrophoresis. SDS denatures cov proteins thiab muab cov nqi tsis zoo rau cov protein; Thaum daim ntawv thov ntawm hluav taws xob teb siv qhov chaw, cov proteins tsiv mus rau qhov zoo davhlau ya nyob twg raws li lawv qhov hnyav molecular. Yog li, hauv kev sib cais, tsuas yog ib qho cuab yeej raug txiav txim siab. Qhov no yog vim li cas qhov no yog hu ua 1D gel electrophoresis.
Daim duab 01: 1D Gel Electrophoresis
Thaum 1D gel electrophoresis, cov proteins raug cais raws li lawv qhov hnyav molecular. Nyob rau hauv no hais txog, qhov qis zog molecules migrate sai nyob rau hauv lub gel piv rau lub siab molecular hnyav proteins. Yog li, cov proteins uas hnyav hnyav nyob ze rau ntawm qhov dej.
2D Gel Electrophoresis yog dab tsi?
2D gel electrophoresis lossis ob-dimensional gel electrophoresis cais cov proteins raws li ob lub zog. Ob lub zog yog lub iso-hluav taws xob taw tes ntawm cov protein thiab molecular hnyav. Txoj kev sib cais ntawm cov protein no ua rau kom muaj kev daws teeb meem ntawm kev sib cais ntawm cov protein. Lub iso-hluav taws xob taw tes ntawm cov protein nyob ntawm pH uas cov protein yog nruab nrab.
Daim duab 02: 2D Gel Electrophoresis
Yog li, hauv 2D gel electrophoresis, cov protein raug tso cai khiav ntawm pH gradient ruaj khov hauv thawj qhov loj me. Hauv qhov thib ob, cov proteins sib cais siv ntsug lossis kab rov tav polyacrylamide gel electrophoresis. Yog li, cov proteins cais raws li lawv qhov hnyav molecular hauv qhov thib ob.
Tsis tas li ntawd, txoj kev no ntawm gel electrophoresis nce qhov kev daws teeb meem ntawm cov protein sib cais. Yog li ntawd, cov protein sib cais yog purer. Txawm li cas los xij, tus nqi ntawm cov txheej txheem yog ntau dua li ib qhov ntev gel electrophoresis.
Dab tsi yog qhov zoo sib xws ntawm 1D thiab 2D Gel Electrophoresis?
- Ob txoj kev cais cov proteins.
- Yog li, lawv tseem ceeb hauv kev ua cov proteins.
Qhov txawv ntawm 1D thiab 2D Gel Electrophoresis yog dab tsi?
Qhov sib txawv tseem ceeb ntawm 1D thiab 2D gel electrophoresis yog 1D gel electrophoresis cais cov proteins raws li qhov hnyav molecular nkaus xwb thaum 2D gel electrophoresis cais cov proteins raws li ob qho tib si iso-hluav taws xob taw tes thiab molecular hnyav. Vim qhov no yooj yim sib txawv ntawm 1D thiab 2D gel electrophoresis, kev daws teeb meem ntawm kev sib cais ntawm cov proteins thiab tus nqi ntawm ob txoj kev kuj txawv.2D gel electrophoresis qhia tau hais tias siab dua 1D gel electrophoresis. Txawm li cas los xij, 2D gel electrophoresis yog kim dua 1D gel electrophoresis.
Hauv qab infographic qhia txog qhov sib txawv ntawm 1D thiab 2D gel electrophoresis.
Summary – 1D vs 2D Gel Electrophoresis
Kev cais cov proteins nyob ntawm ntau yam. 1D gel electrophoresis cais cov proteins raws li qhov hnyav molecular xwb. Txawm li cas los xij, ob qhov loj me lossis 2D gel electrophoresis nce qhov kev daws teeb meem ntawm cov protein sib cais. Tsis tas li ntawd, 2D gel electrophoresis cais cov proteins raws li qhov iso-hluav taws xob taw tes thiab qhov hnyav molecular. Yog li ntawd, cov ntaub ntawv no yog ib qho tseem ceeb rau downstream processing ntawm cov proteins thiab nyob rau hauv lub teb ntawm proteomics. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm 1D thiab 2D gel electrophoresis.
