Qhov Tseem Ceeb - Gel Electrophoresis vs SDS Page
Gel electrophoresis yog cov txheej txheem uas cais cov macromolecules hauv qhov hluav taws xob. Nws yog ib txoj hauv kev hauv Molecular biology cais DNA, RNA thiab cov proteins los ntawm kev sib xyaw ua ke raws li lawv qhov ntau thiab tsawg. SDS Page yog ib hom gel electrophoresis uas yog siv los cais cov proteins los ntawm cov protein sib xyaw raws li lawv qhov ntau thiab tsawg. Gel electrophoresis yog ib lo lus siv los hais txog cov txheej txheem ib txwm siv rau DNA, RNA, thiab protein sib cais thaum SDS Page yog ib hom gel electrophoresis. Qhov no yog qhov sib txawv tseem ceeb ntawm gel electrophoresis thiab SDS Page.
YGel Electrophoresis yog dab tsi?
Gel electrophoresis yog ib qho txheej txheem siv hauv chav kuaj cais cov khoom siv hluav taws xob xws li DNA, RNA, cov protein, thiab lwm yam los ntawm lawv cov khoom sib xyaw. Ib tug gel yog siv nyob rau hauv gel electrophoresis. Nws ua raws li molecular sieve. Muaj ob hom gels siv hauv gel electrophoresis uas yog agarose thiab polyacrylamide. Kev xaiv cov gel thiab gel npaj yog ib qho tseem ceeb uas yuav tsum tau xav txog hauv gel electrophoreses vim qhov pore loj ntawm cov gel yuav tsum tau ua tib zoo tswj kom muaj kev sib cais zoo ntawm cov molecules los ntawm gel electrophoresis. Gel electrophoresis muaj qhov hluav taws xob txuas nrog ob kawg ntawm gel. Ib kawg ntawm cov gel qhia tau hais tias tus nqi zoo thaum lwm qhov kawg yog qhov tsis zoo.
DNA thiab RNA yog qhov tsis zoo them nqi molecules. Thaum lawv tau thauj mus rau hauv cov gel los ntawm qhov tsis zoo kawg ntawm cov gel thiab siv rau hauv hluav taws xob, lawv tsiv los ntawm cov gel pores mus rau qhov zoo kawg nkaus ntawm cov gel. Qhov ceev ntawm kev tsiv teb tsaws nyob ntawm tus nqi thiab qhov loj ntawm cov molecule. Cov me me molecules yooj yim tsiv los ntawm cov gel pores tshaj cov molecules loj. Yog li ntawd, cov molecules me me taug kev deb ntawm cov gel thiab cov molecules loj dua mus deb deb. Txhawm rau soj ntsuam kev mus ncig ntawm cov molecules ntawm cov gel, cov tshuaj tshwj xeeb yog siv. Lub teb hluav taws xob tau thov rau lub sijhawm qee lub sijhawm thiab nres kom tsis txhob poob ntawm cov molecules thiab khaws cov molecules ntawm lawv txoj haujlwm mus ncig. Cov kab sib txawv tuaj yeem pom hauv gel. Cov kab no sawv cev rau cov molecules ntawm ntau qhov sib txawv. Li no, gel electrophoresis yog qhov tseem ceeb rau kev sib txawv ntawm cov molecules raws li lawv qhov ntau thiab tsawg.
Gel electrophoresis tau muab tso rau hauv ntau yam txuj ci ua cov txheej txheem npaj hauv Molecular biology xws li PCR, RFLP, cloning, DNA sequencing, Southern blotting, genome mapping, etc.
Daim duab 01: Agarose gel electrophoresis
SDS Page yog dab tsi?
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS Page) yog ib hom gel electrophoresis siv los cais cov protein. Thaum gel electrophoresis yog siv los cais cov proteins, cov kev kho tshwj xeeb yog xav tau vim cov proteins tsis raug nqi zoo li DNA thiab RNA thiab tsis txhob tsiv mus rau qhov kawg zoo lossis qhov tsis zoo. Yog li ntawd, cov proteins yog denatured thiab coated nrog tus nqi tsis zoo ua ntej gel electrophoresis. Nws yog ua tiav siv cov tshuaj ntxuav tes hu ua sodium dodecyl sulfate (SDS). Cov gel electrophoresis uas siv SDS thiab polyacrylamide gel rau qhov kev txhawb nqa nruab nrab yog hu ua SDS Page. Cov txheej txheem no feem ntau siv hauv biochemistry, noob caj noob ces, forensics thiab molecular biology.
Lub sijhawm SDS Nplooj ntawv, cov proteins sib xyaw nrog SDS. SDS nthuav tawm cov proteins rau hauv cov duab linear thiab coated nrog cov nqi tsis zoo rau lawv cov molecular mass. Vim yog cov nqi tsis zoo, cov protein molecules tsiv mus rau qhov zoo kawg nkaus ntawm cov gel thiab cais raws li lawv cov molecular masses. Hauv SDS Page, polyacrylamide yog siv los ua cov khoom txhawb nqa rau cov gel. Qhov sib cais tiag tiag ntawm cov protein feem ntau yog nyob ntawm cov khoom ntawm gel. Yog li ntawd, kev npaj polyacrylamide gel yuav tsum tau ua tib zoo ua kom zoo thiab qhov tseeb ntawm polyacrylamide yuav tsum tau siv. Polyacrylamide gels qhia kev daws teeb meem siab dua li agarose gels. Li no, SDS Page yog suav tias yog cov txheej txheem daws teeb meem rau kev sib cais cov protein.
SDS Nplooj ntawv yog hom denaturing gel electrophoresis. Nws muaj kev txwv loj hauv kev txheeb xyuas cov protein. Txij li thaum SDS denatures proteins ua ntej kev sib cais, nws tsis tso cai rau kev tshawb pom ntawm enzymatic kev ua si, protein binding kev sib cuam tshuam, protein cofactors, thiab lwm yam.
Daim duab 02: SDS page
Dab tsi yog qhov txawv ntawm Gel Electrophoresis thiab SDS Page?
Gel Electrophoresis vs SDS Page |
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| Gel electrophoresis yog ib txoj hauv kev ua kom cais cov macromolecules siv hluav taws xob. | SDS Nplooj ntawv yog cov txheej txheem siab gel electrophoresis siv los cais cov proteins raws li lawv pawg. |
| Gel Khiav | |
| Nws tuaj yeem ua tau kab rov tav lossis ntsug. | SDS Nplooj ntawv ib txwm khiav vertically. |
| Kev sib cais | |
| Kev sib cais tshwm sim raws li tus nqi thiab qhov loj. | Protein sib cais tshwm sim raws li qhov loj thiab nqi. |
| Kev daws teeb meem | |
| Agarose gel electrophoresis muaj qhov kev daws teeb meem tsawg thiab polyacrylamide gel electrophoresis muaj kev daws teeb meem ntau dua | SDS Nplooj ntawv muaj kev daws teeb meem zoo dua. |
| Denaturation | |
| Gel electrophoresis suav nrog ob qho tib si denaturing thiab tsis denaturing cov txheej txheem. | SDS Nplooj denatures proteins ua ntej kev sib cais. |
Summary – Gel Electrophoresis vs SDS Page
Gel electrophoresis yog ib qho txheej txheem siv rau kev sib cais thiab tshuaj xyuas DNA, RNA thiab cov proteins raws li lawv qhov loj thiab nqi. Muaj ob lub ntsiab ntawm gel electrophoresis uas yog agarose gel electrophoresis thiab polyacrylamide gel electrophoresis. Agarose gels yog siv feem ntau rau kev sib cais nucleic acid; Thaum xav tau kev daws teeb meem siab dua, polyacrylamide gels tau siv. SDS Page yog ib hom gel electrophoresis feem ntau siv los cais cov sib xyaw ua ke ntawm cov protein. Nws yog suav hais tias yog ib qho kev daws teeb meem ntawm kev sib cais protein ntau. Qhov no yog qhov txawv ntawm gel electrophoresis thiab SDS Page.
