Qhov sib txawv tseem ceeb ntawm kev faib tawm ntawm tes thiab centrifugation yog qhov kev faib tawm ntawm tes yog cov txheej txheem ntawm kev sib cais subcellular Cheebtsam, cais cov organelles, thiab sib txawv ntawm lwm cov cellular Cheebtsam, thaum centrifugation yog sub-kauj ruam ntawm cell fractionation, uas muaj feem xyuam rau kev siv ntawm lub centrifugal quab yuam kom sib txawv cellular thiab sub cellular Cheebtsam.
Nyob hauv lub ntiaj teb kev tshawb fawb niaj hnub no, muaj ntau txoj hauv kev los kawm txog cellular Cheebtsam. Yuav kom kawm txog cov cell thiab cellular Cheebtsam (xws li sub-cellular Cheebtsam), nws yog ib qho tseem ceeb rau kev cais thiab sib txawv cellular qauv raws li. Cell fractionation thiab centrifugation yog ob txoj kev koom tes hauv kev sib cais ntawm cov cellular thiab sub-cellular Cheebtsam raws li qhov sib txawv.
Cell Fractionation yog dab tsi?
Cell fractionation yog cov txheej txheem ntawm kev sib cais ntawm cov cell thiab subcellular Cheebtsam, cais organelles, thiab sib txawv lwm yam cellular Cheebtsam. Cell fractionation khaws cia tus kheej ua haujlwm ntawm txhua lub cellular tivthaiv thaum sib cais. Cell fractionation muaj peb theem ua haujlwm: homogenization, pom, thiab centrifugation. Homogenization rhuav tshem cov khoom ntawm tes, thiab thaum pom, nws lim cov homogenate. Centrifugation yog koom nrog hauv kev sib cais cov khoom tawg thiab lim cellular thiab sib txawv.
Daim duab 01: Cell Fractionation
Thaum pib, kev faib tawm ntawm tes tau siv los ua kom pom cov chaw cellular ntawm cov txheej txheem biochemical sib txawv nrog rau kev kuaj mob thiab kev tshawb fawb hauv chav kuaj. Nyob rau hauv lub ntiaj teb niaj hnub no, cov txheej txheem ntawm tes fractionation yog koom nrog cov protein enrichment, protein characterization, thiab protein translocation. Thaum lub sij hawm muaj protein ntau ntxiv, cell fractionation enriches lub hom phiaj proteins thiab txhim kho lub peev xwm los xyuas cov protein ntau tsawg. Thaum lub sij hawm protein characterization, nws txheeb xyuas cov sub-cellular localization ntawm cov proteins, thiab nyob rau hauv protein translocation, cell fractionation pab nyob rau hauv saib xyuas cov translocation ntawm cell signaling molecules.
Centrifugation yog dab tsi?
Centrifugation yog txheej txheem kev siv tshuab uas yog ib kauj ruam ntawm kev faib tawm ntawm tes. Nws suav nrog kev siv lub zog centrifugal los sib txawv ntawm cov cellular thiab sub-cellular Cheebtsam. Kev sib cais ntawm cov khoom tshwm sim raws li qhov loj me, cov duab, qhov ceev, thiab rotor ceev. Thaum lub sij hawm centrifugation, denser cellular Cheebtsam txav deb ntawm lub axis thaum tsawg ntom ntom nti ntawm lub axis. Ua ntej centrifugation, cov khoom raug tshem tawm hauv cov kua tam sim no hauv lub raj centrifuge.
Daim duab 02: Centrifugation
Muaj ntau yam uas cuam tshuam rau centrifugation. Lawv yog qhov ntom ntawm cov qauv thiab nruab nrab, qhov kub thiab txias, viscosity, thiab kev sib hloov ceev. Muaj ntau hom tshuab centrifuge. Lawv yog microcentrifuges, low-speed centrifuges, high-speed centrifuges, thiab ultra-centrifuges. Nyob rau hauv ultra-centrifuges, muaj ob hom: analytical ultracentrifugation thiab preparative ultracentrifugation
Dab tsi yog qhov zoo sib xws ntawm Cell Fractionation thiab centrifugation?
- Cell fractionation thiab centrifugation yog cov txheej txheem tshuab.
- Lawv pab hauv kev sib cais ntawm tes.
- Ob lub xovtooj ntawm tes thiab centrifugation yog qhov tseem ceeb hauv ntau txoj kev kuaj mob.
- Ntxiv mus, lawv xav tau cov tshuab tshwj xeeb thiab khoom siv.
Qhov txawv ntawm Cell Fractionation thiab centrifugation yog dab tsi?
Cell fractionation yog ua tiav cov txheej txheem ntawm kev sib cais cov hlwb, thaum centrifugation yog ib qho txheej txheem uas los ntawm cov xov tooj ntawm tes. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cell fractionation thiab centrifugation. Cov txheej txheem ntawm tes fractionation siv ob qho tib si homogenizer thiab centrifuge, thaum centrifugation tsuas yog siv lub centrifuge. Tsis tas li ntawd, cell fractionation muaj peb theem ua haujlwm: homogenization, pom, thiab centrifugation, tab sis tsis muaj cov kauj ruam uas cuam tshuam rau hauv centrifugation.
Cov hauv qab no qhia txog qhov sib txawv ntawm cov xov tooj ntawm tes thiab centrifugation hauv daim ntawv teev npe rau kev sib piv ntawm ib sab.
Summary – Cell Fractionation vs Centrifugation
Cell fractionation thiab centrifugation yog cov txheej txheem sib cais tshuab. Cell fractionation siv ob homogenizer thiab centrifuge, thaum centrifugation tsuas yog siv lub centrifuge. Cell fractionation yog txheej txheem ntawm kev sib cais subcellular Cheebtsam, cais cov organelles, thiab sib txawv lwm yam cellular Cheebtsam. Centrifugation yog cov txheej txheem kev siv tshuab uas yog cov kauj ruam ntawm kev faib cov xov tooj ntawm tes thiab koom nrog kev siv lub zog centrifugal kom sib txawv ntawm cov cellular thiab sub-cellular Cheebtsam. Yog li, qhov no qhia txog qhov sib txawv ntawm cell fractionation thiab centrifugation.
