Qhov sib txawv tseem ceeb ntawm cell viability thiab cell proliferation yog tias cell viability yog qhov ntsuas ntawm cov cell nyob hauv cov pejxeem, thaum cell proliferation yog ntsuas ntawm cell division.
Ib lub xovtooj yog lub hauv paus tsim kev lom zem ntawm cov khoom muaj sia. Tib neeg lub cev yog tsim los ntawm trillions ntawm cov hlwb. Kev muaj peev xwm los ntsuas qhov tseeb thiab ua tau zoo ntawm cov cell noj qab haus huv yog ib feem tseem ceeb ntawm kev sim kho. Feem ntau txoj hauv kev los ntsuas kev noj qab haus huv ntawm lub xov tooj ntawm tes suav nrog kev ntsuas kev muaj peev xwm, kev loj hlob, apoptosis, thiab autophagy. Cell viability thiab cell proliferation yog ob tug yam ntxwv ntawm lub cell.
Cell Viability yog dab tsi?
Cell viability yog qhov ntsuas ntawm cov cell nyob hauv cov pejxeem. Nws kuj txhais tau tias yog qhov feem pua ntawm cov neeg nyob hauv cov cell cell. Ib lub xovtooj ntawm tes yog suav tias yog qhov siv tau yog tias nws tuaj yeem ua cov txheej txheem metabolic tseem ceeb kom khaws nws cov qauv kev ncaj ncees. Cell viability tuaj yeem ua ib qho kev ntsuas ntawm feem pua ntawm cov hlwb hauv cov cell uas muaj peev xwm faib cov cell.
Daim duab 01: Cell Viability
Viability assay yog ib txoj hauv kev uas tsim los txiav txim siab txog lub peev xwm ntawm cov hlwb kom tswj lossis rov ua kom muaj sia nyob. Lub viability tuaj yeem pom los ntawm lub cev lub cev ntawm cov hlwb. Qee yam ntawm cov khoom no yog kev ua haujlwm txhua yam, kev txav mus los, kev cog lus ntawm cov hlwb, kev ua haujlwm mitotic hauv cellular functions, thiab lwm yam. Tsis tas li ntawd, kev ntsuam xyuas kev muaj peev xwm ua tau zoo dua qub rau kev ntsuas qhov tseem ceeb ntawm lub cev. Lwm yam tshaj li nrhiav tau qhov sib txawv ntawm kev ua neej nyob rau qhov tsis nyob, kev soj ntsuam ntawm cov cell viability kuj tseem tuaj yeem ntsuas qhov ua tiav ntawm cov txheej txheem kab lis kev cai ntawm tes, cov txheej txheem cryopreservation, cov tshuaj toxicity, thiab kev ua haujlwm ntawm cov tshuaj toxicity mitigating tshuaj. Tsis tas li ntawd, qee qhov kev ntsuam xyuas ntawm tes muaj txiaj ntsig muaj xws li kev soj ntsuam ntawm lub sijhawm tiag tiag ntawm cell viability assay, ATP cell viability assay, live-cell viability assay, tetrazolium reduction cell viability assay, thiab resazurin reduction cell viability assay.
Cell Proliferation yog dab tsi?
Cell proliferation yog txhais raws li lub tswv yim uas ib tug cell loj hlob thiab faib los tsim ob tug ntxhais hlwb. Yog li ntawd, cell proliferation yog ntsuas ntawm cell division. Cell proliferation nce tus xov tooj ntawm tes; li no, nws yog ib tug ceev ceev mechanism ntawm cov ntaub so ntswg loj hlob. Cell proliferation ib txwm xav tau kev loj hlob ntawm tes thiab kev faib tawm ntawm tes kom tshwm sim tib lub sijhawm. Yog li ntawd, cell proliferation tsis yog synonymous nrog kev loj hlob ntawm tes los yog cell division.
Daim duab 02: Cell Proliferation
Cov qia hlwb raug kev loj hlob ntawm tes txhawm rau tsim cov kab mob kis mus rau cov menyuam yaus, uas tom qab ntawd sib txawv los tsim cov ntaub so ntswg tshwj xeeb thaum lub sijhawm kev loj hlob. Tag nrho cov naj npawb ntawm cov hlwb hauv cov neeg tuaj yeem txiav txim siab los ntawm kev rho tawm tus nqi ntawm cov cell proliferation los ntawm tus nqi ntawm cov cell tuag. Uncontrolled cell proliferation ua rau mob qog noj ntshav. Kev soj ntsuam kev loj hlob ntawm tes muaj plaub yam tseem ceeb: kev ntsuam xyuas kev ua haujlwm metabolic, kev ntsuam xyuas cell proliferation marker, ATP concentration assay, thiab DNA synthesis assay.
Dab tsi zoo sib xws ntawm Cell Viability thiab Cell Proliferation?
- Cell viability thiab cell proliferation yog ob tug yam ntxwv ntawm tes.
- Ob leeg yog yam tseem ceeb hauv kev ntsuas kev noj qab haus huv ntawm tes.
- Lawv yog qhov tseem ceeb, muaj txiaj ntsig zoo hauv kev sim tshuaj kho.
- Ob tus yam ntxwv tuaj yeem ntsuas los ntawm kev ntsuas tshwj xeeb.
Qhov txawv ntawm Cell Viability thiab Cell Proliferation yog dab tsi?
Cell viability yog qhov ntsuas ntawm cov cell nyob hauv cov pejxeem, thaum cell proliferation yog ntsuas ntawm cell division. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cell viability thiab cell proliferation. Tsis tas li ntawd, kev muaj peev xwm ntawm tes tuaj yeem ntsuas tau los ntawm kev soj ntsuam suav nrog kev soj ntsuam ntawm lub sijhawm tiag tiag ntawm cell viability assay, ATP cell viability assay, live-cell viability assay, tetrazolium reduction cell viability assay, thiab resazurin reduction cell viability assay. Txawm li cas los xij, kev loj hlob ntawm tes tuaj yeem ntsuas tau los ntawm kev ntsuam xyuas xws li kev ntsuam xyuas kev ua haujlwm metabolic, kev ntsuam xyuas cell proliferation marker, ATP concentration assay, thiab DNA synthesis assay.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm cell viability thiab cell proliferation hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Cell Viability vs Cell Proliferation
Cell viability thiab cell proliferation yog ob tug yam ntxwv ntawm tes. Lawv yog ib qho tseem ceeb heev rau kev ntsuas kev noj qab haus huv ntawm tes. Cell viability yog qhov ntsuas ntawm cov cell nyob hauv cov pejxeem, thaum cell proliferation yog ntsuas ntawm cell division. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cell viability thiab cell proliferation.
