Qhov sib txawv tseem ceeb ntawm centrifugation thiab ultracentrifugation yog tias centrifugation siv qis ceev rau cov txheej txheem sib cais, whereas ultracentrifugation siv ceev ceev rau txoj kev sib cais.
Peb tuaj yeem siv nrawm los cais cov khoom sib xyaw hauv cov tshuaj ntsuas sib xyaw. Centrifugation thiab ultracentrifugation yog ob txoj kev sib cais ceev ceev. Centrifugation yog ib qho txheej txheem ntawm kev sib cais cov khoom sib txawv hauv kev sib xyaw ua ke, thaum ultracentrifugation yog cov txheej txheem uas cuam tshuam nrog kev sib cais ntawm cov khoom sib xyaw ua ke raws li lub zog centrifugal uas tsim los ntawm kev sib hloov ceev heev.
Centrifugation yog dab tsi?
Centrifugation yog ib qho txheej txheem ntawm kev sib cais ntawm cov khoom sib txawv hauv qhov sib xyaw ua ke. Txoj kev no suav nrog kev sib hloov ntawm cov qauv nyob ib ncig ntawm lub axis ruaj khov, uas ua rau kev tsim cov centrifugal quab yuam. Lub zog centrifugal ua rau cov khoom hauv cov qauv txav mus los ntawm cov kua nruab nrab. Cov txheej txheem no ua rau cov sedimentation ntawm cov khoom los yog cov hlwb nrog ntau qhov sib txawv thiab qhov ntom ntom ntawm cov nqi sib txawv. Muaj ob hom kev centrifugation loj: qhov sib txawv centrifugation thiab ceev gradient centrifugation.
Differential centrifugation yog cov txheej txheem tshuaj ntsuam xyuas uas peb tuaj yeem cais cov khoom sib xyaw ua ke nyob ntawm qhov loj ntawm cov khoom. Nws yog daim ntawv yooj yim tshaj plaws ntawm centrifugation, thiab peb kuj hu nws txawv pelleting. Txoj kev no yog qhov tseem ceeb hauv kev sib cais cov khoom hauv ib lub cell. Cov khoom uas muaj qhov sib txawv ntawm qhov sib txawv ntawm qhov sib txawv ntawm qhov sib txawv thaum centrifugation. Hauv lwm lo lus, cov khoom loj loj sediment sai dua cov khoom me me. Ntxiv mus, tus nqi sedimentation tuaj yeem nce ntxiv los ntawm kev ua kom lub zog centrifugal.
Daim duab 01: A Centrifuge
Density gradient centrifugation yog ib qho kev tshuaj ntsuam xyuas uas peb tuaj yeem cais cov khoom hauv qhov sib xyaw ua ke raws li qhov ntom ntawm cov khoom. Nyob rau hauv txoj kev no, cov tshuaj yog concentrated nyob rau hauv ib tug daws ntawm cesium ntsev los yog sucrose. Txoj kev muaj feem cuam tshuam ntawm cov khoom raws li buoyancy ceev. Qhov ceev gradient nyob rau hauv txoj kev no yog Cesium ntsev los yog sucrose nruab nrab. Muaj ob hom kev ceev gradient centrifugation: tus nqi-zonal centrifugation thiab isopycnic centrifugation.
Ultracentrifugation yog dab tsi?
Ultracentrifugation yog cov txheej txheem uas cuam tshuam nrog kev sib cais ntawm kev sib xyaw ua ke raws li lub zog centrifugal uas tsim los ntawm kev sib hloov ceev heev, uas feem ntau yog 50, 000 rpm lossis ntau dua. Hauv cov txheej txheem no, cov khoom sib txawv yuav daws tau raws li lawv cov huab hwm coj ntawm qhov sib txawv ceev.
Daim duab 02: Lub Tshuab Ultracentrifuge
Peb tuaj yeem siv ultracentrifuge rau cov txheej txheem ultracentrifugation. Lub tshuab no yog lub centrifuge uas yog optimized rau spinning ib tug rotor ntawm ib tug heev ceev ceev, uas ua rau nws muaj peev xwm ntawm generating acceleration siab li 1,000,000 g. Peb tuaj yeem pom ob hom loj ntawm ultracentrifuges ua kev npaj thiab tshuaj xyuas ultracentrifuge. Ob hom no yog cov cuab yeej tseem ceeb hauv molecular biology, biochemistry, thiab polymer science.
Qhov txawv ntawm Centrifugation thiab Ultracentrifugation yog dab tsi?
Centrifugation yog txheej txheem ntawm kev sib cais ntawm cov khoom sib txawv hauv qhov sib xyaw ua ke. Txawm li cas los xij, ultracentrifugation yog cov txheej txheem uas cuam tshuam nrog kev sib cais ntawm kev sib xyaw ua ke raws li lub zog centrifugal uas tsim los ntawm kev sib hloov ceev heev. Qhov sib txawv tseem ceeb ntawm centrifugation thiab ultracentrifugation yog tias centrifugation siv qhov ceev ceev rau cov txheej txheem sib cais, whereas ultracentrifugation siv ceev ceev rau txoj kev sib cais.
Cov hauv qab no qhia txog qhov sib txawv ntawm centrifugation thiab ultracentrifugation hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Centrifugation vs Ultracentrifugation
Peb tuaj yeem siv nrawm los cais cov khoom sib xyaw hauv cov tshuaj ntsuas sib xyaw. Ob txoj kev sib cais nrawm nrawm yog centrifugation thiab ultracentrifugation. Qhov sib txawv tseem ceeb ntawm centrifugation thiab ultracentrifugation yog tias centrifugation siv ceev ceev rau txoj kev sib cais, thaum ultracentrifugation siv lub siab ceev rau txoj kev sib cais.
