Qhov sib txawv tseem ceeb ntawm polymerase thiab primase yog tias polymerase koom nrog hauv ob qho tib si replication thiab transcription thaum primase koom nrog tsuas yog hauv replication.
Kev rov ua dua ntawm DNA thiab kev sib txuas ntawm cov ntaub ntawv mRNA sib xws ua lub luag haujlwm tseem ceeb hauv cov ntaub ntawv ntws hauv cov kab mob. Ob leeg yog enzyme-mediated functions uas koom nrog polymerization kev ua si. Ntau cov enzymes thiab yam tseem ceeb tau koom nrog hauv kev ua tiav ntawm txhua txoj haujlwm.
YPolymerase yog dab tsi?
Polymerase yog hais txog ib pawg enzymes uas muaj peev xwm ntawm polymerization, i, e, synthesis ntawm cov saw ntev ntawm nucleic acids. Polymerases tuaj yeem muab faib ua pawg raws li cov molecules uas lawv polymerize. Muaj ob lub ntsiab polymerases li DNA polymerase thiab RNA polymerase. Lub luag hauj lwm ntawm DNA polymerase yog thaum lub sij hawm elongation theem ntawm replication, qhov uas nws ntxiv deoxyribonucleotides rau cov strand loj hlob. DNA polymerase ib txwm ntxiv nucleotides hauv 5' rau 3' kev taw qhia. DNA polymerase tsis tuaj yeem pib ntxiv nucleotide; li no nws yuav tsum tau ib tug luv luv RNA template. Tsis tas li ntawd, nws yuav tsum tau clamps kom ruaj khov lawv tus kheej hauv cov saw loj loj.
Daim duab 01: DNA Polymerase
Hauv qhov sib piv, RNA polymerase kev ua haujlwm pib thaum lub sijhawm sau elongation. RNA polymerase ntxiv ribonucleotides hauv 5' rau 3' kev taw qhia. Txawm li cas los xij, RNA polymerases tsis xav tau tus qauv thiab tuaj yeem pib polymerization ntawm nws tus kheej. Hom polymerases txawv ntawm eukaryotes thiab prokaryotes. Txawm li cas los xij, nyob rau hauv tag nrho cov kab mob, lub polymerase enzyme yog ib tug multi-domain enzyme complex.
Primase yog dab tsi?
DNA primase yog ib qho enzyme uas ua lub luag haujlwm tseem ceeb hauv DNA replication. Nws belongs rau ib hom RNA polymerase qhov twg primase catalyzes cov synthesis ntawm luv luv RNA oligonucleotide uas ua raws li tus qauv rau DNA polymerase. Qhov luv luv oligonucleotide no hu ua primer.
Daim duab 02: 3D Qauv ntawm Primase
Thaum khi ntawm RNA primer, DNA polymerase pib nws cov polymerization. RNA primer tom qab raug tshem tawm los ntawm kev ua haujlwm exonuclease. Hauv cov kab mob, cov haujlwm tseem ceeb tshwm sim ua ke nrog DNA helicase. Yog li ntawd, nws tsim ib tug complex helicase los ua ib tug primosome. Archeal thiab eukaryotic primases yog cov protein ntau heterodimeric nrog kev tswj hwm subunits.
Dab tsi yog qhov zoo sib xws ntawm Polymerase thiab Primase?
- Polymerase thiab primase yog cov enzymes uas muaj cov proteins.
- Lawv muaj kev ua haujlwm enzymatic.
- Ob leeg koom nrog hauv cov txheej txheem ntawm DNA replication.
- Ntxiv mus, ob qho enzymes muaj nyob rau hauv ob qho tib si eukaryotes thiab prokaryotes.
- Ob lub enzymes yog hom polymerases.
Qhov txawv ntawm Polymerase thiab Primase yog dab tsi?
Qhov sib txawv tseem ceeb ntawm polymerase thiab primase yog raws li cov txheej txheem lawv tseem ceeb ua. Thaum polymerase plays lub luag haujlwm tseem ceeb hauv ob qho tib si replication thiab transcription, primase yog qhov tseem ceeb uas yuav tsum tau muaj rau cov txheej txheem rov ua dua. Sib nrug ntawm qhov no, hom khoom lawv tsim, lawv lub peev xwm ntawm lawv pib ntawm nws tus kheej, lawv txoj kev koom tes hauv PCR sib txawv ntawm ob hom enzymes.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm polymerase thiab primase hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Polymerase vs Primase
Ob leeg polymerase thiab primase yog cov enzymes uas koom nrog pawg polymerase ntawm cov enzymes. Txawm li cas los xij, qhov sib txawv tseem ceeb ntawm polymerase thiab primase yog nyob ntawm cov txheej txheem lawv tseem ceeb ua. Lub polymerase muaj ntau haiv neeg thiab ua rau ob qho tib si replication thiab transcription txheej txheem. Primase feem ntau yog nyob rau hauv cov txheej txheem replication. Ob leeg yog cov proteins uas qhia txog kev ua haujlwm enzymatic. Ntxiv mus, lawv kuj txawv ntawm cov substrates lawv siv rau polymerization thiab lawv lub peev xwm los pib polymerization ntawm nws tus kheej.
