Qhov sib txawv tseem ceeb ntawm shuttle vector thiab kev qhia vector yog tias shuttle vector feem ntau yog plasmid uas tsis yog tsim los rau kev tshawb fawb txog noob caj noob ces, thaum qhia vector feem ntau yog plasmid lossis kab mob uas yog tsim los rau kev qhia txog noob caj noob ces. cells.
Nyob rau hauv molecular biology, ib tug vector yog ib tug DNA molecule siv los ua lub tsheb nqa txawv teb chaws genetic khoom mus rau lwm lub cell, qhov twg nws yuav replicated los yog qhia. Cov khoom siv caj ces txawv teb chaws hu rau vector feem ntau hu ua recombinant DNA molecule. Plaub hom vectors loj yog plasmids, kab mob vectors, cosmids, thiab cov chromosomes dag. Ntawm cov no, feem ntau siv vectors yog plasmids. Shuttle vector thiab expression vector yog ob hom vectors siv hauv molecular biology.
Shutter Vector yog dab tsi?
A shuttle vector yog vector tsim los nthuav tawm hauv ob hom sib txawv. Yog li ntawd, txawv teb chaws DNA tso rau hauv lub shuttle vector tuaj yeem kuaj lossis kho hauv ob hom cell sib txawv. Feem ntau, lub shuttle vector muaj ob lub hauv paus ntawm kev rov ua dua, txhua qhov tshwj xeeb rau tus tswv tsev. Raws li shuttle vectors replicate nyob rau hauv ob hom sib txawv, lawv kuj hu ua bifunctional vectors. Ib tug nrov shuttle vector yog poov xab shuttle vector. Ntxiv mus, yuav luag tag nrho cov feem ntau siv Saccharomyces cerevisiae vectors yog shuttle vectors. Piv txwv li, cov poov xab shuttle vector muaj cov khoom siv uas tso cai rau kev rov ua dua thiab xaiv hauv ob qho tib si poov xab thiab E. coli hlwb. E.coli Cheebtsam ntawm cov poov xab shuttle vector yog lub hauv paus ntawm replication thiab ib tug xaiv xaiv (e.g., tshuaj tiv thaiv kab mob, beta-lactamases). Cov poov tshuaj ntawm cov poov xab shuttle vector yog autonomously replicating sequence (ARS), ib tug poov xab centromere (CEN), thiab ib tug xaiv poov xab marker (xws li, URA3- ib tug noob uas encodes ib enzyme rau uracil synthesis)
Dab tsi yog kev nthuav qhia vector?
Ib qho kev qhia vector feem ntau yog plasmid lossis tus kab mob tsim los rau cov noob qhia hauv cov hlwb. Cov vector no yog siv los qhia txog cov noob tshwj xeeb rau hauv lub hom phiaj ntawm tes. Cov vector no tuaj yeem tswj hwm lub xovtooj ntawm cov txheej txheem rau kev tsim cov protein kom tsim cov protein encoded los ntawm cov noob tshwj xeeb. Thaum ib qho kev qhia vector nyob rau hauv lub cell, cov protein uas yog encoded los ntawm txawv teb chaws noob yog tsim los ntawm kev siv cellular translational machinery ribosome complexes.
Daim duab 01: Kev nthuav qhia Vector
Cov lus qhia vector yog tsim los kom muaj cov txheej txheem tswj hwm uas ua raws li kev txhawb nqa thiab txhawb nqa cheeb tsam, uas ua rau muaj txiaj ntsig zoo ntawm cov noob txawv teb chaws. Yog li ntawd, ib qho tseem ceeb ntawm cov tub txib ruaj khov RNA (mRNA) tuaj yeem raug muab txhais ua cov protein tshwj xeeb. Kev nthuav qhia ntawm cov protein los ntawm kev siv cov vector vector yuav nruj tswj tau. Cov protein yog tsim nyob rau hauv loj npaum li cas thaum tsim nyog los ntawm kev siv ib tug inducer. Txawm li cas los xij, hauv qee lub cev, cov protein tuaj yeem ua rau pom tau. Ib qho piv txwv nrov rau kev qhia vector yog pCI mammalian vector.
Dab tsi yog qhov zoo sib xws ntawm Shuttle Vector thiab Expression Vector?
- Shuttle vector thiab expression vector yog ob hom vectors siv hauv molecular biology.
- Ob hom vectors tuaj yeem yog plasmids.
- Cov hom vectors muaj keeb kwm ntawm kev rov ua dua.
- Ob hom vectors muaj qhov chaw cloning.
- Cov vectors no muaj cov cim xaiv tau (cov tshuaj tiv thaiv kab mob).
Qhov txawv ntawm Shuttle Vector thiab Expression Vector yog dab tsi?
A shuttle vector feem ntau yog plasmid uas tsis yog tsim los rau kev tshawb fawb noob caj noob ces hauv hlwb, thaum ib qho kev qhia vector feem ntau yog plasmid lossis tus kab mob uas tsim los rau kev tshawb fawb noob hauv hlwb. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm shuttle vector thiab kev qhia vector. Tsis tas li ntawd, lub shuttle vector tsis xav tau kev tswj hwm ib ntus xws li kev txhawb nqa muaj zog, txhim kho, inducer, thiab kev txhais lus txhais lus txav tau yooj yim (PTIS) thiab muaj zog terminator. Ntawm qhov tod tes, kev qhia vector xav tau kev tswj hwm ib ntus xws li kev txhawb nqa muaj zog, txhim kho, inducer thiab portable translation pib ib ntus (PTIS), thiab muaj zog terminator.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm shuttle vector thiab qhia vector hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Shuttle Vector vs Expression Vector
Shuttle vector thiab kev qhia vector yog ob hom vectors siv hauv kev sim molecular lom. Lub shuttle vector feem ntau yog plasmid uas tsis yog tsim los rau cov noob qhia kev tshawb fawb hauv cov hlwb, thaum ib qho kev qhia vector feem ntau yog plasmid lossis tus kab mob uas tsim los rau kev tshawb fawb noob hauv hlwb. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm shuttle vector thiab expression vector.
