Qhov sib txawv tseem ceeb ntawm adeno-associated viral vector thiab adenoviral vector yog tias adeno-associated viral vector yog ib leeg-stranded DNA kab mob vector thaum adenoviral vector yog ob-stranded DNA kab mob vector.
Adeno-txuas kab mob vector thiab adenoviral vector yog ob kab mob. Viral vectors yog cov cuab yeej tseem ceeb heev los xa cov transgenes mus rau cov tswv tsev. Cov txheej txheem no tuaj yeem ua tau nyob rau hauv cov cell muaj sia lossis kab lis kev cai ntawm tes. Cov kab mob muaj ib lub tswv yim tshwj xeeb los thauj lawv cov genomes mus rau hauv lub host cells lawv kis. Kev xa ntawm transgene mus rau ib tus tswv tsev yog hu ua transduction. Molecular biologists thawj zaug siv cov txheej txheem no hauv xyoo 1970s.
Dab tsi yog Adeno-koom nrog Viral Vector?
Adeno-associated viral vector (AAVV) is a single-stranded DNA viral vector. Adeno-associated virus yog tus kab mob DNA me me uas kis tau rau tib neeg thiab lwm hom tsiaj txhu. Nws tsis ua rau muaj kab mob, tab sis nws ua rau lub cev tsis muaj zog heev. Feem ntau, cov kab mob adeno-associated tuaj yeem kis tau cov kab mob sib faib thiab tsis sib faib. Nws muaj peev xwm sib txuas nws cov genome mus rau hauv lub xov tooj ntawm tes. Txawm li cas los xij, nws feem ntau nyob twj ywm li episomal, piv txwv li, rov ua dua yam tsis muaj kev koom ua ke rau hauv chromosome. Yog li, adeno-txuas kab mob vector ua haujlwm ntev thiab ruaj khov ntawm transgenes. Cov yam ntxwv no ua rau tus kab mob adeno-cuam tshuam ib tus kab mob zoo nkauj heev rau kev tshawb fawb molecular lom. Ntxiv mus, tam sim no, nws tau siv los ua tus kab mob kis tau zoo hauv kev kho noob.
Daim duab 01: Adeno-associated Viral Vector
Nws yog ib qho tseem ceeb uas yuav tsum nco ntsoov tias adeno-txuas kab mob vectors tsuas tuaj yeem xa 5 kb transgene rau tus tswv tsev, uas yog qhov me me. Tsis tas li ntawd, tus kab mob no tau ntim ib leeg-stranded DNA thiab xav tau cov txheej txheem ntawm kev sib txuas thib ob. Qhov thib ob strand synthesis slows down qhov qhia nyob rau hauv lub host cell. Yog li ntawd, cov kws tshawb fawb tau ua ib qho kev hloov kho adeno-uas cuam tshuam tus kab mob vector hu ua tus kheej-ua ntxiv adeno-koom nrog tus kab mob kab mob uas ntim ob qho tib si, uas sib koom ua ke los tsim ob-kab DNA. Qhov no tso cai rau cov lus qhia ceev ceev hauv lub xov tooj ntawm tes.
Adenoviral vector yog dab tsi?
Adenoviral vector yog ob-stranded DNA kab mob vector. Adenoviral DNA tsis koom ua ke rau hauv lub genome thiab tsis yog replicated thaum lub sij hawm cell faib. Qhov no txwv lawv txoj kev siv hauv kev tshawb fawb hauv paus. Txawm li cas los xij, qee lub hlwb tshwj xeeb xws li HEK293 tuaj yeem pab txhawb adenovirus replication hauv cov tswv tsev. Thawj daim ntawv thov ntawm adenoviral vectors yog nyob rau hauv kev kho noob thiab txhaj tshuaj tiv thaiv. Muaj ntau qhov zoo ntawm kev siv adenoviral vectors. Qhov loj me me thiab tus cwj pwm zoo genome ntawm adenovirus yog ib qho yooj yim los tswj cov caj ces.
Daim duab 02: Adenoviral Vector
Tsis tas li ntawd, cov kab mob adenoviral muaj kev nyab xeeb, thiab nws kis tau ntau yam kev faib tawm thiab tsis sib faib. Tsis zoo li lentivirus thiab retrovirus, qhov kev pheej hmoo ntawm kev nkag mus mutagenesis yog tsawg dua hauv adenovirus vim nws tsis sib xyaw cov kab mob genome los tuav genome. Qhov teeb meem nkaus xwb nrog cov vector no yog neutralizing antibodies uas inactivate lawv nyob rau hauv hosts zoo li tib neeg. Chimpanzee adenovirus vector tau txais txiaj ntsig zoo rau cov tshuaj tiv thaiv kab mob no. Chimpanzee adenovirus vector yog cov kab mob adenoviral uas paub zoo, uas yog siv los ua tus vector los thauj cov SARS-COV-2 cov kab mob loj hlob hauv Oxford AstraZeneca COVID tshuaj tiv thaiv.
Dab tsi yog qhov zoo sib xws ntawm Adeno-associated Viral Vector thiab Adenoviral Vector?
- Adeno-txuas kab mob vector thiab adenoviral vector yog ob kab mob.
- Ob leeg yog cov kab mob uas tsis yog lub hnab.
- Lawv yog DNA-based.
- Lawv tuaj yeem kis mus rau kev dhia dej thiab tsis sib cais.
- Ntxiv mus, lawv tuaj yeem rov ua dua-tsis zoo.
- Ob tus kab mob kis tau siv rau hauv kev kho noob.
Dab tsi yog qhov txawv ntawm Adeno-associated Viral Vector thiab Adenoviral Vector?
Ib qho adeno-txuas kab mob vector yog ib leeg-stranded DNA kab mob vector, thaum lub adenoviral vector yog ob-stranded DNA kab mob vector. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm adeno-txuas kab mob vector thiab adenoviral vector. Ntxiv mus, Adeno-txuas nrog tus kab mob no tsuas tuaj yeem xa 5 kb txawv teb chaws DNA mus rau lub xov tooj ntawm tes thaum lub adenoviral vector tuaj yeem xa mus txog 36 kb txawv teb chaws DNA rau cov tswv tsev.
Cov infographic hauv qab no teev qhov sib txawv ntawm adeno-txuas kab mob vector thiab adenoviral vector nyob rau hauv daim ntawv tabular rau ib sab sib piv.
Summary – Adeno-associated Viral Vector vs Adenoviral Vector
Viral vectors yog cov cuab yeej siv tau zoo los xa cov transgenes rau cov tswv tsev. Adeno-associated viral vector thiab adenoviral vector yog ob hom kab mob uas nrov heev tam sim no siv hauv kev kho noob. adeno-associated viral vector yog ib leeg-stranded DNA kab mob vector thaum lub adenoviral vector yog ob-stranded DNA kab mob vector. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm adeno-associated viral vector thiab adenoviral vector.
