Qhov sib txawv tseem ceeb ntawm guanidine thiocyanate thiab guanidine hydrochloride yog guanidine thiocyanate yog cov protein ntau dua denaturant uas feem ntau siv hauv RNA cais, thaum guanidine hydrochloride yog cov protein tsis muaj zog denaturant uas tsis tshua siv hauv RNA cais.
Denaturation yog cov txheej txheem uas cov proteins poob lawv cov qauv quaternary, tertiary qauv, thiab cov qauv theem nrab uas ib txwm muaj nyob hauv lawv lub xeev. Nws tuaj yeem ua tau los ntawm kev siv qee qhov kev ntxhov siab sab nraud lossis cov tebchaw xws li cov kua qaub lossis lub hauv paus, cov ntsev tsis muaj zog, cov organic hnyav (cawv, chloroform), agitation, hluav taws xob, lossis cua sov. Guanidine thiocyanate thiab guanidine hydrochloride yog ob hom protein denaturants.
Guanidine Thiocyanate yog dab tsi?
Guanidine thiocyanate (GTC) yog ib qho muaj zog protein denaturing tus neeg sawv cev uas feem ntau siv hauv RNA cais. Nws tseem hu ua guanidinium isothiocyanate (GITC). Ua tus neeg sawv cev chaotropic, nws yog cov tshuaj sib xyaw ua ke niaj hnub siv los ua cov protein ntau denaturant. Tus neeg sawv cev chaotropic yog cov molecule hauv cov kua dej uas tuaj yeem cuam tshuam cov hydrogen bonding network ntawm cov dej molecules. Qhov no muaj kev cuam tshuam rau kev ruaj ntseg ntawm haiv neeg lub xeev ntawm lwm cov molecules hauv cov tshuaj, xws li macromolecules zoo li cov proteins thiab nucleic acids los ntawm kev ua kom tsis muaj zog hydrophobic. Chaotropic cov neeg ua haujlwm zoo li guanidine thiocyanate txo qhov kev txiav txim hauv cov qauv ntawm cov protein tsim los ntawm cov dej molecules ob qho tib si hauv cov khoom loj thiab cov hydration shells nyob ib ncig ntawm hydrophobic amino acids. Qhov no yuav ua rau muaj protein ntau denaturation.
Guanidine thiocyanate tuaj yeem siv los tua cov kab mob xws li mob npaws uas ua rau muaj kab mob xws li mob khaub thuas Spanish xyoo 1918. Yog li ntawd, nws tuaj yeem siv rau hauv kev kho mob lossis tsev kho mob teeb tsa. Tsis tas li ntawd, Guanidine thiocyanate kuj tseem siv los ua lyse hlwb thiab cov kab mob hauv RNA thiab DNA extractions. Ntawm no, kev ua haujlwm ntawm guanidine thiocyanate yog los pab lysing kev ua haujlwm thiab tiv thaiv kev ua haujlwm ntawm RNase enzymes thiab DNase enzymes los ntawm denaturing. Cov enzymes no yuav ua rau cov extract (RNA lossis DNA).
Guanidine Hydrochloride yog dab tsi?
Guanidine hydrochloride (GdnHCl) yog ib qho tsis muaj protein ntau denaturant uas tsis tshua siv hauv RNA cais. Nws tseem hu ua guanidinium chloride (GdmCl). Nws yog hydrochloride ntsev ntawm guanidine. Guanidine hydrochloride yog chaotropic thiab ib qho ntawm cov denaturants siv nyob rau hauv physicochemical kev tshawb fawb ntawm protein folding.
Daim duab 01: Guanidine Hydrochloride
Guanidine hydrochloride kuj muaj peev xwm txo qis kev ua haujlwm ntawm enzyme thiab ua rau kom muaj kev sib luag ntawm hydrophobic molecules. Feem ntau, ntawm qhov siab dua ntawm guanidine hydrochloride, cov proteins poob lawv cov qauv txiav txim. Proteins zoo li ua random coiled ntawm qhov concentration ntawm guanidine hydrochloride. Hauv kev kho mob lossis tsev kho mob teeb tsa, guanidine hydrochloride yog qhia rau txo qis cov tsos mob ntawm cov leeg tsis muaj zog thiab yooj yim fatigability cuam tshuam nrog tus kab mob Eaton-Lambert syndrome. Tsis tas li ntawd, cov kev mob tshwm sim ntawm kev siv guanidine hydrochloride tuaj yeem suav nrog peristalsis, raws plab, thiab kev ua rau cov pob txha tuag.
Dab tsi zoo sib xws ntawm Guanidine Thiocyanate thiab Guanidine Hydrochloride?
- Guanidine thiocyanate thiab guanidine hydrochloride yog ob hom protein denaturants.
- Ob leeg denaturants muaj guanidine hauv lawv cov qauv.
- Lawv yog cov neeg ua haujlwm chaotropic.
- Ob lub denaturants tuaj yeem siv rau kev cais RNA.
- Lawv ob leeg siv rau hauv kev kho mob lossis tsev kho mob teeb tsa.
Qhov txawv ntawm Guanidine Thiocyanate thiab Guanidine Hydrochloride yog dab tsi?
Guanidine thiocyanate yog ib qho muaj zog protein ntau denaturant tus neeg sawv cev uas feem ntau siv nyob rau hauv RNA kev rho tawm, thaum guanidine hydrochloride yog cov protein tsis muaj zog denaturant uas tsis tshua siv hauv RNA cais. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm guanidine thiocyanate thiab guanidine hydrochloride. Tsis tas li ntawd, cov tshuaj formula ntawm guanidine thiocyanate yog C2H6N4S, thaum cov tshuaj formula. ntawm guanidine hydrochloride yog CH5N3HCl.
Cov duab hauv qab no nthuav tawm qhov sib txawv ntawm guanidine thiocyanate thiab guanidine hydrochloride hauv daim ntawv tabular rau ib sab sib piv.
Summary – Guanidine Thiocyanate vs Guanidine Hydrochloride
Guanidine thiocyanate thiab guanidine hydrochloride yog ob hom protein denaturants. Ob lub denaturants yog chaotropes. Guanidine thiocyanate yog ib tug muaj zog protein denaturant tus neeg saib xyuas uas yog feem ntau siv nyob rau hauv RNA cais, thaum guanidine hydrochloride yog ib tug tsis muaj zog protein denaturant uas tsis tshua muaj siv nyob rau hauv RNA cais. Yog li, qhov no qhia txog qhov sib txawv ntawm guanidine thiocyanate thiab guanidine hydrochloride.
