Qhov sib txawv tseem ceeb ntawm NeuN thiab MAP2 yog NeuN yog ib qho protein coded los ntawm RBFOX3 noob, thaum MAP2 yog protein coded los ntawm MAP2 noob.
Neuronal markers yog pab tau rau kev txheeb xyuas cov neuronal. Lawv feem ntau yog qhov tseem ceeb hauv kev paub qhov txawv neurons los ntawm lwm lub hlwb hlwb, txiav txim siab txog tus kheej neuronal, txhais cov neuron txoj haujlwm, thiab tsim cov koom tes sib koom ua ke. Muaj ntau cov cim neuronal, xws li cov cim tsis paub qab hau (NCAM), cov cim paub tsis meej neuronal (NeuN, MAP2, beta-III tubulin), cov cim ua haujlwm ntawm lub paj hlwb, (Chat, tyrosine hydroxylase), thiab cov cim neuronal synaptic (PSD-95, synaptophysin).).
NeuN yog dab tsi?
NeuN yog cov protein uas tau muab los ntawm RBFOX3 noob uas feem ntau siv los ua cov cim biomarker neuronal. NeuN los yog RBFOX3 yog ib qho ntawm peb tus tsiaj txhu ntawm RNA binding protein ntawm RBFOX noob tsev neeg. Tag nrho cov proteins ntawm RBFOX noob tsev neeg tau koom nrog hauv kev tswj hwm ntawm lwm txoj kev RNA splicing. Keeb kwm, nws tau raug txheeb xyuas tias yog ib qho epitope NeuN uas nyob hauv thaj tsam N davhlau ya nyob twg ntawm RBFOX3 protein. RBFOX cov neeg hauv tsev neeg tau txais txiaj ntsig zoo, muaj ib qho RNA paub txog motif (RRM) hom RNA binding domain (RBD) ze rau qhov nruab nrab ntawm cov protein ntau. RBFOX3 protein ntau tau txais txiaj ntsig zoo thoob plaws tib neeg, nas, thiab nas. Cov protein no koom nrog kev tswj hwm ntawm lwm txoj kev sib txuas ntawm cov neeg hauv tsev neeg nrog rau hauv autoregulation. Lub hlwb thiab cov leeg nqaij tshwj xeeb lub hom phiaj rau lwm txoj kev sib txuas yog tsim tau zoo rau RBFOX proteins.
Daim duab 01: NeuN
Qhov kev qhia ntawm NeuN / RBFOX3 protein yog txwv rau lub paj hlwb thiab tau siv dav hauv kev tshawb fawb mob stroke. Yog li ntawd, nws tau lees paub tias yog ib qho cim ntawm kev paub tab ntawm cov kab mob hlwb hauv qaum qaum, cerebral cortex, hippocampus, dorsal thalamus, caudate / putamen, thiab cerebellum. NeuN protein tuaj yeem kuaj pom siv RBFOX3 / NeuN antibody los ntawm kev tiv thaiv kab mob. NeuN yog cov protein uas yog homologue rau cov khoom lag luam protein ntawm cov noob caj noob ces hauv Caenorhabditis elegans.
MAP2 yog dab tsi?
Microtubule-associated protein 2 lossis MAP2 yog cov protein uas siv los ua cov cim paub txog neuronal. Cov gene MAP2 codes rau cov protein no. MAP2 belongs rau MAP2/Tau tsev neeg. Nws muaj plaub isoforms li MAP2a, MAP2b, MAP2c, thiab MAP2d. MAP2 isoforms yog txuam nrog microtubules thiab kho lawv cov kev cuam tshuam nrog actin filaments, yog li ua si lub luag haujlwm tseem ceeb hauv kev teeb tsa microtubule-actin network. Tsis tas li ntawd, MAP2 isoforms tau txhim kho kev tswj hwm thiab sib txawv hauv cov neurons thiab qee lub hlwb glial.
Daim duab 02: MAP2
MAP2c tau qhia nyob rau hauv lub hlwb tsim, thiab lwm yam isoforms qhia nyob rau hauv cov neeg laus lub hlwb. Kev faib tawm ntawm MAP2 isoforms kuj txawv. MAP2a thiab MAP2b yog nyob rau hauv dendrites, thaum MAP2c pom nyob rau hauv axons. Kev qhia ntawm MAP2d tsis txwv rau cov neurons thiab tuaj yeem pom hauv glia, xws li oligodendrocytes. MAP2 tau lees paub tias yog tus cim tseem ceeb ntawm cov hlwb paub tab thiab tuaj yeem kuaj pom los ntawm MAP2 cov tshuaj tiv thaiv los ntawm kev tiv thaiv kab mob.
Qhov Zoo sib xws ntawm NeuN thiab MAP2 yog dab tsi?
- NeuN thiab MAP2 yog ob tus neeg paub txog cov kab mob neuronal.
- Ob leeg yog cov proteins ua los ntawm cov amino acids.
- Lawv tau hais tawm hauv cov hlwb paub tab.
- Ob leeg cov protein tuaj yeem kuaj pom los ntawm cov tshuaj tiv thaiv tshwj xeeb los ntawm kev tiv thaiv kab mob.
- Lawv ua haujlwm tseem ceeb hauv lub cev.
Qhov txawv ntawm NeuN thiab MAP2 yog dab tsi?
NeuN yog cov protein coded los ntawm RBFOX3 noob, thaum MAP2 yog protein coded los ntawm MAP2 noob. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm NeuN thiab MAP2. Tsis tas li ntawd, qhov hnyav molecular ntawm NeuN yog 46 kDa, thaum lub cev hnyav ntawm MAP2 yog 199 kDa.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm NeuN thiab MAP2 hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – NeuN vs MAP2
Neuronal markers muaj txiaj ntsig zoo rau kev txheeb xyuas cov hlwb neuronal. NeuN thiab MAP2 yog ob qho kev paub txog neuronal. NeuN yog protein encoded los ntawm RBFOX3 noob, thaum MAP2 yog protein encoded los ntawm MAP2 noob. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm NeuN thiab MAP2.
