Qhov sib txawv tseem ceeb ntawm Km thiab Vmax yog Km ntsuas qhov yooj yim npaum li cas ntawm enzyme tuaj yeem saturated los ntawm substrate, whereas Vmax yog qhov siab tshaj plaws ntawm cov enzyme yog catalyzed thaum lub enzyme yog saturated los ntawm substrate.
Km tuaj yeem piav qhia raws li kev sib tham ntawm lub substrate uas ib nrab ntawm qhov siab tshaj plaws tau ua tiav. Vmax, ntawm qhov tod tes, tuaj yeem piav qhia raws li cov tshuaj tiv thaiv ntawm lub xeev qhov twg cov enzyme yog tag nrho saturated los ntawm substrate.
Km yog dab tsi?
Km tuaj yeem piav qhia raws li kev sib tham ntawm lub substrate uas ib nrab ntawm qhov siab tshaj plaws tau ua tiav. Hauv lwm lo lus, nws yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax. Yog li ntawd, ib qho enzyme uas muaj qhov siab Km qhia tau tias tsis muaj kev sib raug zoo rau nws cov substrate. Nws kuj yuav tsum muaj ntau dua ntawm cov substrate kom ua tiav Vmax.
Lub sijhawm Km hauv kev ua enzyme tau tham hauv qab Michaelis-Menten kinetics. Nws yog ib qho qauv ntawm enzyme kinetics. Cov qauv no tau muaj npe tom qab German biochemist Leonor Michaelis thiab Canadian tus kws kho mob Maud Menten. Cov qauv no tau qhia raws li qhov sib npaug.
v=d[P]/dt=Vmax([S]/Km+[S])
Hauv qhov sib npaug saum toj no, Vmax yog qhov siab tshaj plaws ua tiav los ntawm lub kaw lus uas tshwm sim ntawm qhov saturated substrate concentration rau qhov muab enzyme concentration. Km yog tus Michaelis tas mus li. Yog hais tias nws yog tus lej sib npaug ntawm cov substrate concentration, ces cov tshuaj tiv thaiv yog ib nrab ntawm tus nqi ntawm Vmax.
Ntxiv mus, biochemical cov tshuaj tiv thaiv nrog ib lub substrate feem ntau xav tias qhia Michaelis-Menten kinetics yam tsis muaj kev txhawj xeeb ntawm qhov kev xav ntawm tus qauv no.
Vmax yog dab tsi?
Vmax tuaj yeem piav qhia raws li cov tshuaj tiv thaiv ntawm lub xeev qhov twg cov enzyme yog tag nrho saturated los ntawm substrate. Lub xeev no qhia tau hais tias txhua qhov chaw sib txuas tau raug rov ua dua tshiab. Hauv lwm lo lus, Vmax yog qhov siab tshaj plaws cov tshuaj tiv thaiv lossis tshaj tawm ntawm cov tshuaj tiv thaiv uas yog enzymatically catalyzed thaum lub saturation ntawm cov enzyme nrog nws cov substrate.
Nws yog ib qho tseem ceeb los txiav txim siab Km thiab Vmax rau qee yam kev ua haujlwm enzymatic vim tias kev paub txog cov txiaj ntsig no tso cai rau peb los kwv yees txoj hmoo ntawm metabolic ntawm substrate thiab tus txheeb ze ntawm cov substrate uas yuav dhau los ntawm txhua txoj hauv kev nyob rau hauv cov xwm txheej sib txawv. Tus nqi qis dua ntawm Vmax qhia tau hais tias cov enzyme ua haujlwm hauv cov xwm txheej zoo.
Qhov txawv ntawm Km thiab Vmax yog dab tsi?
Cov ntsiab lus Km thiab Vmax yog qhov tseem ceeb hauv cov enzyme kinetics. Qhov sib txawv tseem ceeb ntawm Km thiab Vmax yog Km ntsuas yooj yim npaum li cas cov enzyme tuaj yeem saturated los ntawm substrate, whereas Vmax yog qhov siab tshaj plaws tus nqi ntawm cov enzyme yog catalyzed thaum lub enzyme yog saturated los ntawm substrate.
Cov lus hauv qab no qhia txog qhov sib txawv ntawm Km thiab Vmax.
Summary – Km vs Vmax
Km yog kev sib koom ua ke ntawm cov substrate uas ib nrab ntawm qhov siab tshaj plaws tau ua tiav. Vmax yog cov tshuaj tiv thaiv tus nqi ntawm lub xeev qhov twg enzyme yog tag nrho saturated los ntawm substrate. Qhov sib txawv tseem ceeb ntawm Km thiab Vmax yog Km ntsuas yooj yim npaum li cas cov enzyme tuaj yeem saturated los ntawm substrate, qhov Vmax yog qhov siab tshaj plaws ntawm cov enzyme yog catalyzed thaum lub enzyme saturated los ntawm substrate.
