Qhov sib txawv tseem ceeb ntawm lub xeev khov kho thiab lub sijhawm daws teeb meem fluorescence yog qhov ruaj khov-xeev fluorescence cuam tshuam nrog kev kawm txog qhov nruab nrab ntev fluorescence ntawm tus qauv thaum irradiated nrog UV, pom lossis ze IR teeb, thaum lub sijhawm daws teeb meem fluorescence koom nrog kev kawm txog cov fluorescence ntawm cov qauv uas tau soj ntsuam raws li kev ua haujlwm ntawm lub sijhawm tom qab excitation los ntawm lub teeb mem tes.
Fluorescence tuaj yeem txhais tau tias pom lossis pom tsis pom hluav taws xob uas tawm los ntawm cov khoom vim qhov xwm txheej hluav taws xob ntawm lub voj voog luv luv. Hauv lwm lo lus, nws yog emissions ntawm lub teeb los ntawm ib yam khoom uas muaj absorbed lub teeb los yog lwm yam EMR (electromagnetic hluav taws xob). Qhov piv txwv tshaj plaws rau fluorescence yog qhov nqus ntawm cov hluav taws xob hauv cheeb tsam UV ntawm cov spectrum los ntawm cov qauv (uas yog qhov tsis pom ntawm peb) thiab emitting lub teeb nyob rau hauv cheeb tsam pom. Qhov no muab cov qauv sib txawv xim uas pom tau tsuas yog thaum lub teeb UV. Ntxiv mus, cov ntaub ntawv fluorescent zoo li ci ntsa iab yuav luag tam sim ntawd thaum tshem tawm cov hluav taws xob hluav taws xob.
Ntse-state Fluorescence yog dab tsi?
Ib lub xeev cov fluorescence tsis tu ncua yog cov txheej txheem tshuaj ntsuam xyuas uas kawm txog qhov nruab nrab ntev ntawm cov fluorescence ntawm tus qauv raws li qhov irradiation ntawm tus qauv nrog UV, pom, lossis ze IR teeb. Cov ntawv thov ntawm cov fluorescence tsis tu ncua muaj xws li excitation thiab emission scans, synchronous scans thiab maps, khov kho-state fluorescence anisotropy, excitation-emission maps, kinetic ntsuas, thiab kub maps.
Lub Sijhawm daws teeb meem Fluorescence yog dab tsi?
Lub sijhawm daws teeb meem fluorescence yog cov txheej txheem tshuaj ntsuam xyuas uas kawm txog cov fluorescence ntawm cov qauv uas tau saib xyuas raws li lub sijhawm ua haujlwm tom qab lub zog ntawm lub teeb mem tes. Peb tuaj yeem muab nws lub npe txuas ntxiv ntawm fluorescence spectroscopy. Nyob rau hauv cov txheej txheem no, peb yuav tsum tau soj ntsuam ib tug qauv (tom qab nws excitation los ntawm ib tug flash ntawm lub teeb) raws li ib tug muaj nuj nqi ntawm lub sij hawm.
Muaj ntau txoj hauv kev uas peb tuaj yeem tau txais lub sijhawm daws teeb meem fluorescence, suav nrog cov khoom siv hluav taws xob ceev ceev, lub sijhawm sib cuam tshuam ib leeg-photon suav, lub koob yees duab streak, lub koob yees duab CCD hnyav, qhov rooj kho qhov muag, thiab lwm yam. Thaum lub sijhawm- daws teeb meem fluorescence, lub convolution integral yog siv los xam lub neej ntawm fluorescence lwj.
Qhov txawv ntawm Lub Xeev thiab Lub Sijhawm daws Fluorescence yog dab tsi?
Fluorescence tuaj yeem txhais tau tias pom lossis pom tsis pom hluav taws xob uas tawm los ntawm cov khoom vim qhov xwm txheej hluav taws xob ntawm lub voj voog luv luv. Muaj ob qhov derivatives ntawm fluorescence li khov kho-lub xeev thiab lub sij hawm daws teeb meem fluorescence. Qhov sib txawv tseem ceeb ntawm lub xeev khov kho thiab lub sij hawm daws teeb meem fluorescence yog tias cov fluorescence tsis tu ncua muaj kev kawm txog cov fluorescence mus sij hawm ntev ntawm cov qauv thaum irradiated nrog UV, pom lossis ze IR teeb, thaum lub sij hawm daws teeb meem fluorescence cuam tshuam nrog kev kawm ntawm fluorescence ntawm tus qauv uas tau saib xyuas raws li kev ua haujlwm ntawm lub sijhawm tom qab lub zog los ntawm lub teeb mem tes.
Ib lub xeev cov fluorescence khov kho yog siv rau hauv kev tshawb fawb thiab kev tso tawm, synchronous scans thiab maps, ruaj khov-state fluorescence anisotropy, excitation-emission maps, kinetic ntsuas, thiab kub maps. Lub sij hawm daws teeb meem fluorescence, ntawm qhov tod tes, yog siv hauv TR-FRET systems (lub sij hawm daws teeb meem fluorescence zog hloov)
Cov lus hauv qab no qhia txog qhov sib txawv ntawm lub xeev khov kho thiab lub sijhawm daws teeb meem fluorescence.
Summary – Steady State vs Time Resolved Fluorescence
Lub fluorescence khov kho thiab lub sijhawm daws teeb meem fluorescence yog qhov tseem ceeb heev hauv kev tshuaj ntsuam xyuas thiab kev siv lub cev. Qhov sib txawv tseem ceeb ntawm lub xeev ruaj khov thiab lub sij hawm daws teeb meem fluorescence yog qhov ruaj khov fluorescence cuam tshuam nrog kev kawm txog qhov nruab nrab fluorescence mus sij hawm ntev ntawm tus qauv thaum irradiated nrog UV, pom lossis ze IR teeb, thaum lub sij hawm daws teeb meem fluorescence cuam tshuam nrog Kev kawm txog fluorescence ntawm tus qauv uas tau soj ntsuam raws li kev ua haujlwm ntawm lub sijhawm tom qab lub zog los ntawm lub teeb mem tes.
