Qhov sib txawv tseem ceeb ntawm CHO-S thiab CHO-K1 yog qhov uas cov hlwb loj hlob thiab loj hlob. Thaum CHO-S tau yoog rau kev loj hlob hauv cov kab lis kev cai raug tshem tawm, CHO-K1 yog cov kab xov tooj ntawm tes uas tuaj yeem tsim cov noob caj noob ces kom loj hlob raws li cov hlwb raug tshem tawm lossis cov hlwb ua ke.
CHO sawv cev rau Suav Hamster Ovary cells. Nws yog cov kab mob epithelial. Cov kab mob epithelial no yog muab los ntawm lub zes qe menyuam ntawm Suav hamster. CHO yog siv rau hauv kev tshawb fawb txog kev kho mob thiab tshuaj lom neeg. Nws kuj tseem siv dav hauv kev tsim cov khoom lag luam muaj kev kho mob recombinant proteins. Cov kev tshawb fawb tshawb fawb uas CHO thiab CHO-derived cell kab tau siv suav nrog kev tshawb fawb hauv kev tshuaj ntsuam toxicity, kev tshawb nrhiav caj ces, khoom noj khoom haus, thiab cov noob qhia txog kev qhia txog cov proteins sib xyaw.
CHO-S yog dab tsi?
CHO-S yog hom kab ntawm cov kab mob uas tau muab los ntawm CHO cell kab. Qhov tseem ceeb ntawm CHO-S cov hlwb hauv kev tshawb fawb kev tshawb fawb yog kev hloov kho ntawm cov kab ntawm tes hauv cov kab lis kev cai uas tsis muaj cov tshuaj tiv thaiv kab mob kom loj hlob mus rau cov cell ceev. Li no, CHO-S cell kab yog qhov zoo tagnrho rau kev loj hlob ntawm kev lag luam bioreactors loj. Lawv yog cov tseem ceeb hauv cov txheej txheem ntawm cov protein qhia hauv biomanufacturing. Kev loj hlob ntawm CHO-S cell kab nyob rau hauv serum-free suspension nyob ntawm ntau yam. Lawv yog cov ntaub ntawv xov xwm, kev saib xyuas ntawm tes kom zoo, thiab hom kab lis kev cai ntawm tes. Tam sim no, CHO-S cov hlwb siv cov ntawv xov xwm niaj hnub no rau kev loj hlob. Cov no suav nrog Kev Tshaj Tawm Nruab Nrab los ntawm Mirus Bio thiab CHOgro®. Cov xov xwm no tso cai rau CHO-S cov hlwb kom ncav cuag lub xovtooj ntawm 1-2 × 10 7hlwb / ml. Ntxiv rau hom xov xwm saum toj no, ob peb lwm hom xov xwm, suav nrog Hams's F10, Ham's F12, ProCHO, thiab PowerCHO™ rau kev loj hlob CHO-S hlwb pom zoo.
Figure 01: CHO
Qhov tsuas yog qhov tsis zoo ntawm kev siv hom xov xwm no yog qhov CHO-S cov hlwb sai sai noj cov khoom noj muaj nyob hauv nruab nrab. Yog li, txhawm rau kov yeej qhov kev sib tw no rau kev saib xyuas ntawm tes, qhov kev ncua yuav tsum tau muab faib ua kom qis qis ntawm tes thiab muab qhov nruab nrab loj hlob tshiab tsis tu ncua.
CHO-K1 yog dab tsi?
CHO-K1 yog ib kab kab ntawm tes muab los ntawm CHO cell kab ua subclone los ntawm niam txiv CHO cell kab muab los ntawm zes qe menyuam ntawm tus neeg laus Suav hamster. Nws yog ib qho tseem ceeb kom muab cov proline ua ib qho ntxiv rau kev loj hlob nruab nrab thaum loj hlob CHO-K1 hlwb. Qhov no yog vim qhov tseeb tias CHO-K1 hlwb tsis muaj chromosome uas tsim nyog rau proline biosynthesis. Qhov no ua rau muaj kev cuam tshuam ntawm cov saw biosynthetic thaum lub sij hawm hloov ntawm glutamic acid rau glutamine gamma seri-aldehyde.
CHO-K1 hlwb tau siv dav hauv kev tshawb fawb txog kev noj zaub mov thiab noob caj noob ces, kab lis kev cai ntawm tes, kev loj hlob, kev hloov pauv ntawm tes ruaj khov, hloov pauv hloov pauv, thiab qhia cov protein. CHO-K1 hlwb loj hlob raws li cov hlwb raug tshem tawm lossis cov hlwb txuas ntxiv tom qab kev tswj cov caj ces. Yog li, CHO-K1 hlwb tau siv dav hauv kev kho cov protein ntau thiab cov kev tshawb fawb hauv vitro cancer ntsig txog qog noj ntshav ntawm zes qe menyuam. CHO-K1 hlwb ua rau pom kev kis tus kab mob thiab pom kev tiv thaiv kab mob poliovirus. CHO-K1 hlwb kuj ua raws li tus tswv tsev qhia qhov system rau kev loj hlob yam, monoclonal antibodies, interferons, thiab enzymes. Lub luag haujlwm ntawm CHO-K1 cov hlwb hauv cov haujlwm no yog qhov tseem ceeb.
Qhov Zoo sib xws ntawm CHO-S thiab CHO-K1 yog dab tsi?
- CHO-S thiab CHO-K1 yog ob lub xovtooj ntawm tes muab los ntawm Suav Hamster Ovarian epithelial cells.
- Ob hom yog loj hlob nyob rau hauv ncua kev kawm ntawv.
- Cov hom no yog siv rau hauv kev tshawb fawb txog kev noj qab haus huv thiab kev kho mob.
- Ntxiv mus, ob qho tib si CHO-S thiab CHO-K1 yog siv nyob rau hauv ntau yam kev lag luam muaj kev tshawb fawb kho mob.
Qhov txawv ntawm CHO-S thiab CHO-K1 yog dab tsi?
Qhov sib txawv tseem ceeb ntawm CHO-S thiab CHO-K1 yog tias CHO-S loj hlob raws li cov hlwb raug tshem tawm, tab sis CHO-K1 loj hlob raws li ob qho tib si ncua thiab cov hlwb. Ob qho CHO-S thiab CHO-K1 yog muab los ntawm tib yam poj koob yawm txwv CHO tab sis muaj qhov sib txawv thaum nws los txog rau cov ntawv thov. CHO-K1 tsis muaj proline-synthesizing noob, tsis zoo li CHO-K1. Li no, proline yog ntxiv rau hauv xov xwm thaum culturing.
Cov ntawv qhia hauv qab no nthuav tawm qhov sib txawv ntawm CHO-S thiab CHO-K1 hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – CHO-S vs CHO-K1
CHO-S thiab CHO-K1 cell kab yog muab los ntawm Suav Hamster Ovary (CHO) epithelial hlwb. Ob hom yog qhov tseem ceeb rau ntau yam kev tshawb fawb kev tshawb fawb, ob qho tib si kho mob thiab lom. CHO-S yog cov kab hluav taws xob ncua, thaum CHO-K1 yog ib qho kev ncua lossis cov kab txuas txuas ntxiv. Qhov tseem ceeb ntawm CHO-K1 yog tias nws tsis muaj proline-synthesizing noob. CHO-S yog dav siv nyob rau hauv industrial bioreactor systems. CHO-K1 daim ntawv thov dag ntau ntawm cov kev tshawb fawb gene qhia. Yog li, qhov no qhia txog qhov sib txawv ntawm CHO-S thiab CHO-K1.
