Qhov txawv ntawm PARP1 thiab PARP2 yog cov nroj tsuag thiab tsiaj txhu PARP1 nqa Zn-ntiv tes DNA khi motifs thaum cog PARP2 nqa N-terminal SAP DNA khi motifs.
Poly ADP ribose polymerase (PARP) yog tsev neeg cov proteins uas yog cov enzymes nuclear. Muaj 17 ntau hom PARP enzymes hauv PARP tsev neeg. PARP1 thiab PARP2 yog ob lub enzymes tseem ceeb rau kev kho DNA ib txwm muaj.
PARP yog dab tsi?
PARP (Poly ADP ribose polymerase) yog tsev neeg ntawm cov proteins uas ua haujlwm hauv ntau cov txheej txheem ntawm tes xws li DNA kho, genomic stability, thiab programmed cell tuag. Lawv catalyze tus txheej txheem hu ua ADP-ribosylation. ADP-ribosylation yog hais txog qhov sib ntxiv ntawm ADP-ribose units los ntawm nicotinamide adenine dinucleotide (NAD) rau lub hom phiaj substrates txhawm rau kho DNA strand so.
PARP enzymes yog DNA-binding proteins. Lawv tau qhib los ntawm cov nicks tam sim no hauv DNA molecules. Thaum lawv khi nrog DNA tawg, lawv hydrolyze NAD+ rau hauv nicotinamide thiab txhawb cov polymerization ntawm ADP-ribose. Yog li, PARP nuclear enzymes koom nrog hauv DNA kho. Tsis tas li ntawd, poly-ADP-ribosylation ua haujlwm raws li kev hloov pauv tom qab kev txhais lus uas ua lub luag haujlwm tseem ceeb hauv kev rov ua dua, kev tswj hwm kev hloov pauv, kev saib xyuas telomere thiab degradation protein. PARP enzymes kuj koom nrog hauv kev saib xyuas ntawm genome stability, kev tswj ntawm chromatin qauv, cell proliferation, thiab apoptosis.
PARP1 yog dab tsi?
PARP1 yog ib tug tswv cuab ntawm PARP protein tsev neeg. Nws yog thawj thiab zoo tshaj plaws-tus yam ntxwv protein nyob rau hauv tsev neeg no. Nws ua raws li tus neeg teb thawj zaug uas kuaj pom DNA puas thiab tom qab ntawd pab txhawb kev xaiv kho cov txheej txheem. Ntxiv mus, PARP1 tswj kev kho ntawm ib leeg-strand DNA puas.
Daim duab 01: PARP1
Ntxiv rau kev kho ib leeg-strand DNA so, PARP1 tswj kev rov ua dua rab diav rawg thiab rov pib dua. Tsis tas li ntawd, nws txhawb nqa lwm txoj kev uas tsis yog homologous kawg-joining.
PARP2 yog dab tsi?
PARP2 yog lwm tus tswvcuab protein hauv PARP protein tsev neeg. Nws muaj feem xyuam nrog PARP1 enzyme. Cov noob PARP2 codes rau PARP2 protein nyob rau hauv tib neeg. PARP2 muaj lub catalytic domain tab sis tsis muaj N davhlau ya nyob twg DNA binding domain. PARP inhibitor tshuaj tiv thaiv kab mob qog noj ntshav tuaj yeem cuam tshuam PARP2, zoo ib yam li PARP1.
Daim duab 02: PARP2
Nyob hauv cov nroj tsuag, tshwj xeeb tshaj yog nyob rau hauv Arabidopsis thaliana, PARP2 plays lub luag haujlwm tseem ceeb hauv kev tiv thaiv cov lus teb rau DNA puas thiab kab mob pathogenesis tshaj PARP1.
Qhov Zoo sib xws ntawm PARP1 thiab PARP2 yog dab tsi?
- PARP1 thiab PARP2 yog ob hom nuclear enzymes.
- Lawv siv NAD + ua lawv cov substrate los catalyze poly ADP ribosylation.
- Ob leeg yog qhib los ntawm DNA ib leeg-strand so.
- Mammalian PARP1 thiab PARP2 nyob rau hauv lub nucleus.
- Ob leeg PARP1 thiab PARP2 cuam tshuam nrog chromatin.
- Lawv kho DNA puas.
- Qee PARP inhibitor tshuaj tiv thaiv kab mob qog noj ntshav tsom rau PARP1 kuj inhibit PARP2.
Qhov txawv ntawm PARP1 thiab PARP2 yog dab tsi?
PARP1 yog ib tug tswv cuab ntawm PARP protein tsev neeg uas muaj cov catalytic domain thiab N-terminal DNA binding domain thaum PARP2 yog ib tug tswv cuab ntawm PARP tsev neeg uas tsis muaj N-terminal DNA binding domain. Qhov sib txawv tseem ceeb ntawm PARP1 thiab PARP2 yog cov nroj tsuag thiab tsiaj txhu PARP1 nqa Zn-ntiv tes DNA khi motifs thaum cog PARP2 nqa N-terminal SAP DNA binding motifs.
Cov duab hauv qab no qhia txog qhov sib txawv ntawm PARP1 thiab PARP2.
Summary – PARP1 vs PARP2
PARPs yog cov enzymes nuclear uas paub txog DNA puas thiab kho lawv. PARP1 thiab PARP2 yog ob ntawm lawv uas muaj feem cuam tshuam zoo. Ob lub enzymes muaj lub luag haujlwm catalytic. Tab sis PARP2 tsis muaj N-terminal DNA binding domain uas muaj nyob rau hauv PARP1. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm PARP1 thiab PARP2. Ob hom enzymes siv NAD+ ua lawv cov substrate.
