Key Difference – DMEM vs EMEM
Tsiaj cell culturing yog ua los tswj tsiaj cell kab thiaj li ua tau ntau yam kev tshawb fawb. Tsiaj cov kab ntawm tes raug tswj xyuas kom tsis muaj menyuam, thiab lawv xav tau cov txheej txheem tshwj xeeb. Lawv tau siv rau hauv kev tshawb fawb txog kev tsim tshuaj tiv thaiv, txhawm rau txheeb xyuas tus cwj pwm ntawm cov cell mus rau carcinogens thiab mutagens thiab hauv kev tshawb fawb txog qog noj ntshav. Cov xov xwm kab lis kev cai ntawm tes yog qhov tseem ceeb heev rau kev vam meej ntawm cov kab lis kev cai ntawm cov tsiaj. Muaj ntau hom kab lis kev cai ntawm tes xws li Dulbecco's Modified Eagle Medium (DMEM) thiab Eagle's Minimum Essential Medium (EMEM). DMEM yog hloov kho basal nruab nrab nrog nce amino acid thiab vitamin concentrations mus txog plaub npaug. Qhov no kuj suav nrog qee qhov kev hloov pauv ntxiv uas ua rau cov khoom noj muaj txiaj ntsig ntawm kev tshaj tawm. EMEM yog ib qho ntawm thawj hom tsiaj kab lis kev cai xov xwm uas tsim los ntawm Harry Eagle. Nws yog ib qho yooj yim, basal media nrog qhov tsawg kawg nkaus ntawm cov khoom noj muaj pes tsawg leeg. Qhov sib txawv tseem ceeb ntawm ob qhov kev tshaj tawm yog cov khoom noj muaj pes tsawg leeg. EMEM yog tsim los ntawm qhov tsawg kawg nkaus ntawm cov as-ham uas yuav tsum tau rau kev loj hlob ntawm kab lis kev cai, whereas DMEM yog ib tug complex xov xwm uas muaj ntau ntau ntawm cov amino acids thiab vitamins.
DMEM yog dab tsi?
Dulbecco's Modified Eagle's Medium (DMEM) yog hom xov xwm hloov kho uas tau ua lag luam npaj ua cov hmoov dawb dawb. Qhov no tau yoog los ntawm EMEM, thiab cov khoom noj muaj pes tsawg leeg tau hloov kho los ntawm kev nce cov ntsiab lus ntawm cov amino acids thiab cov vitamins. Qhov concentration ntawm cov amino acids yog nce mus txog ob zaug nyob rau hauv kev sib piv rau basal nruab nrab. Cov vitamin concentration yog nce mus txog plaub npaug yog li ua kom cov ntsiab lus hauv nruab nrab.
DMEM kuj tau hloov kho los ntawm kev ntxiv ntsev ntxiv xws li ferric nitrate, sodium pyruvate thiab qee qhov tsis tseem ceeb amino acids xws li serine thiab glycine. Qhov concentration ntawm qabzib hauv xov xwm kuj hloov pauv. Tus thawj formulation yog tsim los ntawm 1000 mg / L ntawm qabzib, whereas nyob rau hauv DMEM, lub concentration yog nce mus rau 4500 mg / L. DMEM kuj tseem yuav tsum tau ntxiv cov kua dej nruab nrab vim nws tsis yog qhov nruab nrab tiav. Feem ntau, DMEM yog ntxiv nrog Fetal Bovine Serum (FBS). FBS muab cov proteins uas yuav tsum tau muaj thiab kev loj hlob ntawm cov txheej txheem culturing.
Cov pH ntawm nruab nrab sib txawv raws li qhov sib ntxiv ntawm Sodium Bicarbonate. pH ntawm nruab nrab ua ntej ntxiv Sodium Bicarbonate yog nyob ib ncig ntawm 6.80 - 7.40, whereas pH, tom qab ntxiv Sodium Bicarbonate nyob rau hauv thaj tsam ntawm 7.60 - 8.20. Qhov ntsuas kub ntawm cov xov xwm yog 2 – 8 0C.
Daim duab 01: DMEM
Kev thov ntawm DMEM
- Kawm kawm txog cov quav hniav tsim muaj peev xwm ntawm polyomavirus hauv nas embryonic hlwb.
- Nyob rau hauv kev sib cuag inhibition kev tshawb fawb
- In chicken cell cultures
EMEM yog dab tsi?
Eagle Qhov Tsawg Qhov Tseem Ceeb Qhov Nruab Nrab (EMEM) yog ib qho ntawm thawj qhov kev tshaj tawm hauv xov tooj ntawm tes ntawm cov tsiaj cell kab. Thawj hom tsiaj kab mob uas siv EMEM suav nrog nas L hlwb thiab HeLa hlwb. EMEM media kuj yog ib hom kev hloov kho xov xwm. Harry Eagle thawj zaug tsim EMEM nruab nrab. EMEM media suav nrog cov amino acids tseem ceeb thiab cov vitamins hauv qhov tsawg kawg nkaus xav tau los ntawm cov hom cell. Cov amino acids tsis tseem ceeb tsis suav nrog hauv kev tsim, thiab cov piam thaj thiab sodium bicarbonate concentrations raug txo. Txawm hais tias cov xov xwm muaj qhov sib npaug ntawm qhov kev loj hlob yam tsawg kawg nkaus rau kev loj hlob ntawm cov hlwb.
EMEM tsis yog qhov nruab nrab tiav. Yog li ntawd, supplementation nrog serum yog yuav tsum tau rau txoj kev loj hlob ntawm cov mammalian hlwb. EMEM yog siv rau ntau lub hlwb thiab tseem yog cov xov xwm nrov ntawm cov kws tshawb fawb kab lis kev cai.
Dab tsi zoo sib xws ntawm DMEM thiab EMEM?
- Ob hom xov xwm yog siv nyob rau hauv tsiaj cell kab lis kev cai.
- Ob hom xov xwm yog cov qauv ua kua.
- Ob hom xov xwm yog hloov kho cov xov xwm los ntawm cov xov xwm basal.
- Ob hom xov xwm muaj cov amino acids tseem ceeb, cov vitamins thiab inorganic ntsev uas xav tau rau kev loj hlob.
- Ob hom xov xwm tsis tiav. Yog li ntawd, cov tshuaj yuav tsum tau ntxiv.
- Ob hom xov xwm siv cov piam thaj los ua nws qhov chaw carbon.
- Ob hom xov xwm muaj pH siab dua thiab raug kho los ntawm kev ntxiv sodium bicarbonate.
Qhov txawv ntawm DMEM thiab EMEM yog dab tsi?
DMEM vs EMEM |
|
| DMEM yog ib hom kev hloov kho basal nruab nrab, nrog nce amino acid thiab vitamin ntau ntxiv. Qhov no kuj suav nrog qee qhov kev hloov pauv ntxiv uas ua rau cov khoom noj muaj txiaj ntsig ntawm kev tshaj tawm. | EMEM yog thawj hom tsiaj kab lis kev cai xov xwm tsim los ntawm Harry Eagle. Nws yog ib qho yooj yim, basal media nrog qhov tsawg kawg nkaus ntawm cov khoom muaj pes tsawg leeg. |
| Hloov kho ntawm Amino Acid | |
| Amino acid concentration tau nce mus txog ob npaug hauv DMEM nruab nrab. | Tsawg concentration ntawm amino acid yog siv hauv EMEM. |
| kev hloov kho ntawm cov vitamins | |
| Vitamin concentration tau nce mus txog plaub npaug hauv DMEM. | Tsawg concentration ntawm cov vitamins yog siv hauv EMEM. |
| Glucose Concentration | |
| Glucose concentration tau nce mus txog 4500 mg / L hauv DMEM. | Glucose concentration yog 1000 mg/L hauv EMEM. |
| Kev muaj tsis tseem ceeb Amino Acids | |
| Present in DMEM. | Absent in EMEM. |
| Nyob ntawm cov ntsiab lus ntxiv | |
| Cov khoom xws li ferric nitrate, sodium pyruvate muaj nyob hauv DMEM. | EMEM muaj cov khoom noj tsawg kawg nkaus. |
Summary – DMEM vs EMEM
DMEM thiab EMEM yog ob hom tsiaj kab lis kev cai xov xwm uas feem ntau sib txawv hauv lawv cov khoom noj khoom haus. DMEM yog hloov kho daim ntawv ntawm EMEM, qhov twg cov khoom noj muaj zog ntxiv nrog rau kev ntxiv ntawm qee yam tshiab. EMEM yog cov xov xwm me me thiab muaj tag nrho cov yam tseem ceeb uas yuav tsum tau muaj rau kev loj hlob ntawm cov tsiaj cell kab. Cov xov xwm no yuav tsum tau siv nyob rau hauv cov neeg mob tsis muaj menyuam, thiab ob qho tib si xov xwm yuav tsum tau ntxiv nrog cov ntshav ua ntej siv. Qhov no yog qhov txawv ntawm DMEM thiab EMEM.
Download PDF of DMEM vs EMEM
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm DMEM thiab EMEM
