Qhov Tseem Ceeb Qhov txawv - RPMI vs DMEM
Txoj kev tshawb fawb feem ntau cuam tshuam txog kev cog qoob loo ntawm cov tsiaj thiab yog li, cov kev tshawb fawb no xav tau kev saib xyuas cov kab ntawm cov tsiaj hauv cov kab mob tshwj xeeb. Tsiaj cell culturing yog ua tau dav hauv kev tshawb fawb pharmacological los txheeb xyuas cov tshuaj metabolism hauv kev kuaj kab mob thiab hauv kev tshawb fawb txog qog noj ntshav. Lub cell kab lis kev cai xov xwm yog ib qho tseem ceeb ntawm cov tsiaj cell kab lis kev cai. Dulbecco's Modified Eagle Medium (DMEM) thiab Roswell Park Memorial Institute Medium (RPMI) yog ob kab lis kev cai xov xwm siv nyob rau hauv tsiaj cell culturing. RPMI yog ib qho kev tshaj tawm uas siv dav rau kab lis kev cai ntawm cov tsiaj txhu hauv kab lis kev cai. DMEM yog ib hom kev hloov kho basal nruab nrab, nrog rau cov amino acid thiab cov vitamin ntau ntxiv mus txog plaub npaug. DMEM yog siv rau hauv kev cog qoob loo hauv cov kab lis kev cai. Qhov sib txawv tseem ceeb ntawm ob lub xov xwm yog hom kab lis kev cai ntawm kev tshaj xov xwm. RPMI yog siv rau kev ncua kev coj noj coj ua, qhov DMEM yog siv rau kab lis kev cai ntawm cov hlwb.
RPMI yog dab tsi?
RPMI lossis Roswell Park Memorial Institute Medium kuj raug hu ua RPMI 1640. Lub npe ntawm cov xov xwm tau muab los ntawm lub koom haum uas tshawb pom cov xov xwm. Cov xov xwm no feem ntau yog siv nyob rau hauv tsiaj cell kab lis kev cai, tshwj xeeb tshaj yog rau kev loj hlob ntawm mammalian hlwb. Lawv yog thawj zaug tsim los loj hlob tib neeg lymphocytes.
Cov khoom xyaw hauv qab no suav nrog RPMI.
- YGlucose
- ntsuas pH (phenol red)\
- Cov ntsev (sodium chloride, sodium bicarbonate, disodium phosphate, potassium chloride, magnesium sulfate, thiab calcium nitrate)
- Amino acids
- Vitamins (i-inositol, choline chloride, para-aminobenzoic acid, folic acid, nicotinamide, pyridoxine hydrochloride, thiamine hydrochloride, calcium pantothenate, biotin thiab riboflavin, cyanocobalamin)
Qhov tshwj xeeb ntawm RPMI yog qhov nce ntxiv ntawm phosphate hauv nruab nrab. RPMI yog siv nyob rau hauv 5% carbon dioxide cua. Qhov no yuav muab qhov siab tshaj plaws thiab kev pom zoo rau kev loj hlob ntawm tes. Cov pH ntawm cov xov xwm yog khaws cia ntawm 8.0 los ntawm bicarbonate buffering system.
Daim duab 01: Cell Culture on a Petri Dish
Kev thov ntawm RPMI hauv Cell Culturing
- Kev cog qoob loo ntawm tib neeg T thiab B lymphocytes, pob txha pob txha hlwb thiab hybridoma.
- Siv los kawm tib neeg cov hlwb neoplastic.
DMEM yog dab tsi?
Dulbecco's Modified Eagle's Medium (DMEM) yog qhov hloov kho nruab nrab uas tau npaj ua lag luam. Thawj qhov kev ntxhib los mos ntawm cov hmoov nruab nrab yog daj. Nyob rau hauv DMEM, vitamin concentration yog nce mus txog plaub npaug li ua rau cov ntsiab lus ntawm cov as-ham hauv nruab nrab.
DMEM kuj tau hloov kho los ntawm kev ntxiv ntsev ntxiv xws li ferric nitrate, sodium pyruvate thiab qee qhov tsis tseem ceeb amino acids xws li serine thiab glycine. Kev tsim cov piam thaj hauv cov xov xwm kuj hloov pauv. Tus thawj formulation yog tsim los ntawm 1000 mg / L ntawm qabzib, whereas nyob rau hauv DMEM, lub concentration yog nce mus rau 4500 mg / L. DMEM kuj tseem yuav tsum tau ntxiv cov kua dej nruab nrab vim nws tsis yog qhov nruab nrab tiav. Feem ntau, DMEM yog ntxiv nrog Fetal Bovine Serum (FBS). FBS muab cov proteins uas yuav tsum tau muaj thiab kev loj hlob ntawm cov txheej txheem culturing.
Cov pH ntawm nruab nrab sib txawv raws li qhov sib ntxiv ntawm Sodium Bicarbonate. pH ntawm nruab nrab ua ntej ntxiv Sodium Bicarbonate yog nyob ib ncig ntawm 6.80 - 7.40, whereas pH tom qab ntxiv Sodium Bicarbonate nyob rau hauv thaj tsam ntawm 7.60 - 8.20. Qhov ntsuas kub ntawm qhov nruab nrab yog 2 - 8 0C.
Daim duab 02: DMEM
Kev thov ntawm DMEM
- Kawm kawm txog cov quav hniav tsim muaj peev xwm ntawm polyomavirus hauv nas embryonic hlwb.
- Nyob rau hauv kev sib cuag inhibition kev tshawb fawb.
- Nyob rau hauv kev tshawb fawb thiab tshuaj xyuas qaib cell kab lis kev cai.
Dab tsi yog qhov zoo sib xws ntawm RPMI thiab DMEM?
- Ob leeg RPMI thiab DMEM yog siv nyob rau hauv tsiaj cell kab lis kev cai.
- Ob leeg RPMI thiab DMEM media yog cov qauv ua kua.
- Ob leeg RPMI thiab DMEM cov xov xwm muaj cov amino acids tseem ceeb, cov vitamins thiab inorganic ntsev xav tau rau kev loj hlob.
- Ob leeg RPMI thiab DMEM xov xwm tsis tiav. Yog li ntawd, cov tshuaj yuav tsum tau ntxiv.
- Ob leeg RPMI thiab DMEM media siv cov piam thaj los ua nws cov pa roj carbon.
- Ob leeg RPMI thiab DMEM media muaj pH siab dua.
Qhov txawv ntawm RPMI thiab DMEM yog dab tsi?
RPMI vs DMEM |
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| RPMI yog ib qho nruab nrab siv dav rau kab lis kev cai ntawm cov tsiaj txhu hauv kab lis kev cai. | DMEM yog ib hom kev hloov pauv ntawm basal nruab nrab nrog nce amino acid thiab vitamin concentrations. |
| Muaj Cov Phosphates Ntxiv | |
| Tam sim no hauv RPMI. | Absent in DMEM. |
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Siv rau kab lis kev cai hauv cov kab lis kev cai. Nws siv los,
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| Yam kab lis kev cai | |
| RPMI yog siv rau kev ncua kev coj noj coj ua. | DMEM yog siv rau cov kab lis kev cai nrog cell adhered. |
Summary – RPMI vs DMEM
Ob leeg RPMI thiab DMEM tau siv dav hauv cov tsiaj cell culturing tshwj xeeb tshaj yog rau kab lis kev cai tsiaj cell kab nrog rau cov lymphocytes hauv vitro. DMEM yog ib qho kev hloov kho basal nruab nrab uas muaj cov khoom noj muaj zog ntxiv. RPMI tsim los ntawm Roswell Park Memorial lub koom haum, thiab nws kuj yog ib qho nruab nrab siv nyob rau hauv cov tsiaj cell culturing tshwj xeeb tshaj yog rau mammalian lymphocytes. Ob leeg xov xwm yog kev lag luam. Qhov no yog qhov txawv ntawm RPMI thiab DMEM.
