Qhov sib txawv tseem ceeb ntawm HPLC thiab HPLC ceev yog tias lub twj tso kua mis uas peb siv rau HPLC yog nyob ib ncig ntawm 40 MPa whereas lub twj tso kua mis siab ceev HPLC yog nyob ib ncig ntawm 3-5 MPa.
Cov txheej txheem feem ntau uas peb siv rau kev sib cais cov khoom sib xyaw yog "kua chromatography". Muaj ntau ntau txoj hauv kev kom ua tiav lub hom phiaj no uas HPLC nrov heev. HPLC stands rau High Performance Liquid Chromatography uas txheeb xyuas, ntsuas thiab cais cov khoom ntawm cov kua sib tov kom nrawm thiab ua tau zoo. Fast HPLC yog ib daim ntawv thov tshwj xeeb ntawm HPLC uas tau lig ntes tau cov neeg koom nrog hauv kev kawm txog kev daws teeb meem.
HPLC yog dab tsi?
HPLC yog qhov ua tau zoo ua kua chromatography. Nws ua rau kev siv lub zog siab los thawb cov kuab tshuaj kom lawv hla mus rau ib kem. Tsis tas li, nws yog ib txoj hauv kev zoo tshaj plaws los cais thiab kawm ntau yam sib txuas xws li amino acids, nucleic acids, carbohydrates, steroids, tshuaj tua kab mob thiab hydrocarbons. HPLC muaj ntau yam zoo dua lwm txoj hauv kev sib cais ntawm cov sib tov xws li kev daws teeb meem siab dua thiab kev soj ntsuam sai dua. Nws obliterates qhov xav tau rau repacking thiab regeneration thiab muaj kev tswj kom zoo dua tsis tau thiab yog li, ua kom efficiency ntawm kev sib cais. Qhov zoo tshaj plaws, HPLC haum rau cov ntawv thov loj vim nws tuaj yeem cais cov khoom me me thiab tso cai rau kev siv cov kev kub ntxhov uas ua rau nws yooj yim rau cov kuab tshuaj ntws yooj yim.
Daim duab 01: HPLC Kem
Hauv luv luv, txoj kev ua haujlwm ntawm HPLC cov txheej txheem yog raws li hauv qab no. Ua ntej, peb yuav tsum qhia tus qauv mus rau hauv cov kwj ntawm lub sijhawm mobile hauv kev sib cais me me uas siv lub twj tso kua mis. Lub twj tso kua mis siab yuav tsum tau khaws cia ntawm 40 MPa. Cov kwj ntawm theem mobile no percolates los ntawm kem ntawm HPLC. Tom qab ntawd, cov khoom hauv cov qauv kuj dhau los ntawm kab. Txawm li cas los xij, lawv qhov nrawm ntawm kev txav txawv ntawm ib leeg vim qhov sib txawv ntawm kev sib cuam tshuam ntawm cov qauv piv txwv thiab cov adsorbent hauv kab. Peb hu cov adsorbent no ua lub sijhawm nyob ruaj khov vim nws nyob hauv kem (tsis txav). Tom qab ntawd, thaum kawg ntawm kab, peb tuaj yeem sau cov qauv uas elutes los ntawm kem, yog li, peb tuaj yeem sau cov khoom hauv cov qauv cais.
HPLC ceev yog dab tsi?
Tsis ntev los no, cov kws tshawb fawb tshawb fawb ntawm Schering Plow lub koom haum tshawb fawb tau tsim cov txheej txheem HPLC ceev uas yog qib siab ntawm HPLC vim nws tso cai rau kev sib cais sai ntawm cov tebchaw los ntawm kev sib tov. Tsis tas li ntawd, lub npe hu ua HPLC ceev, cov txheej txheem no ua rau siv 2.7um fused core silica hais uas yog tsim los ntawm fusing 0.5um ntxeem tau silica tshaj cov khoom silica. Cov khoom sib txawv no ua rau cov txheej txheem ntawm kev sib cais ceev heev thiab qhov ntawd tsis tshua muaj zog dua li qhov peb siv hauv HPLC.
Daim duab 02: FPLC Apparatus
Hnub HPLC tau txais txiaj ntsig piv nrog UPLC, tab sis cov kws tshawb fawb xav tias cov txheej txheem no zoo dua vim nws tsis xav tau cov cuab yeej siv siab siab heev. Qhov zoo tshaj plaws yog qhov ceev HPLC tsis tas yuav muaj cov txheej txheem kuaj tshiab.
Qhov txawv ntawm HPLC thiab HPLC ceev yog dab tsi?
HPLC yog qhov ua tau zoo ua kua chromatography thiab nrawm HPLC yog ib qho kev ua haujlwm siab ntawm cov kua chromatography. Qhov sib txawv tseem ceeb ntawm HPLC thiab HPLC ceev yog tias lub twj tso kua mis siab uas peb siv rau HPLC yog nyob ib ncig ntawm 40 MPa. Whereas, lub twj tso kua mis siab ceev HPLC yog nyob ib ncig ntawm 3-5 MPa. Tsis tas li ntawd, peb tuaj yeem cais cov khoom sib txawv xws li amino acids, nucleic acids, carbohydrates, steroids, tshuaj tua kab mob thiab hydrocarbons siv cov txheej txheem HPLC thaum HPLC cov txheej txheem ceev yog tsuas yog siv rau kev sib cais ntawm cov protein.
Cov ntawv qhia hauv qab no nthuav tawm cov ntaub ntawv ntxiv ntawm qhov sib txawv ntawm HPLC thiab HPLC ceev.
Summary – HPLC vs Fast HPLC
HPLC yog cov txheej txheem ntawm cov kua chromatography uas tau nrov heev thoob plaws ntiaj teb. Fast HPLC yog ib qho tshiab ntawm HPLC, cov kws tshawb fawb tshawb fawb ntawm Schering Plow Institute tsim cov txheej txheem tshiab no. Qhov sib txawv tseem ceeb ntawm HPLC thiab ceev HPLC yog nyob ntawm lub twj tso kua mis uas peb siv rau kev sib cais. Qhov ntawd yog; Lub twj tso kua mis siab uas peb siv rau HPLC yog nyob ib ncig ntawm 40 MPa. Tab sis, lub twj tso kua mis siab ceev HPLC yog nyob ib ncig ntawm 3-5 MPa.
