Qhov sib txawv tseem ceeb ntawm xylene cyanol thiab bromophenol xiav yog tias hauv 1% agarose gel, xylene cyanol migrates qeeb, whereas bromophenol xiav migrates sai.
Xylene cyanol thiab bromophenol xiav yog qhov tseem ceeb ua cov cim xim uas tuaj yeem siv los saib xyuas cov txheej txheem ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis.
YXylene Cyanol yog dab tsi?
Xylene cyanol yog cov xim electrophoretic los yog cov xim taug qab. Nws yog qhov tseem ceeb hauv kev saib xyuas cov txheej txheem ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis. Thaum cov khoom no sib xyaw nrog cov qauv, qhov concentration ntawm cov khoom no feem ntau yog li ntawm 0.005% rau 0.03%. Qee lub ntsiab lus uas peb tuaj yeem siv rau xylene cyanol suav nrog Acid Blue 147, xylene cyanole, xylene cyanol FF, xylene cyanole FF, thiab lwm yam.
Cov tshuaj formula ntawm cov tshuaj no yog C25H27N2NaO 6S2 Cov molar mass ntawm xylene cyanol yog 538.61 g / mol. Cov tshuaj no tuaj yeem txav mus los ntawm tus nqi kwv yees li 4 - 5 kilobase khub DNA tawg hauv 1% agarose gels. Txawm li cas los xij, qhov no nyob ntawm qhov tsis sib xws uas peb siv. Feem ntau, xylene cyanol ntawm 6% polyacrylamide gel tuaj yeem txav mus los ntawm qhov nrawm ntawm li 140 lub hauv paus khub DNA tawg. Ntawm qhov tod tes, nws tuaj yeem txav mus rau hauv 20% denaturing polyacrylamide gel electrophoresis ntawm tus nqi ntawm 25 bases oligonucleotide.
Lub hydrogen daim ntawv cog lus pub suav ntawm xylene cyanol yog 2m, thiab cov hydrogen daim ntawv lees txais suav yog 8. Lub rotatable bond suav ntawm cov compound no tuaj yeem muab tau raws li 7. Qhov nyuaj ntawm xylene cyanol tuaj yeem piav raws li 1120 degrees. Nws muaj ib daim ntawv cog lus stereocenter suav ntawm 1.
Bromophenol Blue yog dab tsi?
Bromophenol xiav yog qhov ntsuas pH muaj txiaj ntsig uas muaj txiaj ntsig zoo li cov xim electrophoretic thiab xim xim. Nws muaj cov tshuaj npe 3', 3', 5', 5'-tetrabromophenolsulfonphthalein, BPB. Cov tshuaj formula ntawm no compound yog C19H10Br4O5 S. Cov molar loj ntawm cov tshuaj no yog 669.96 g / mol. Nws tsis muaj ntxhiab, thiab nws qhov ntom tuaj yeem muab tau li 2.2 g / mL. Nws cov ntsiab lus melting yog 273 degrees Celsius, thiab nws qhov kub ntawm 279 degrees Celsius. Peb tuaj yeem npaj nws los ntawm maj mam ntxiv cov bromine ntau dhau rau cov tshuaj kub ntawm phenolsulfonphthalein hauv glacial acetic acid.
Bromophenol xiav yog qhov tseem ceeb ua cov kua qaub-puag ntsuas hauv thaj tsam ntawm pH 3.0 txog 4.6. Nws muaj peev xwm hloov los ntawm daj ntawm pH 3.0 mus rau pH 4.6. Ntxiv mus, nws yog ib tug reversible tshuaj. Structureally, bromophenol xiav zoo ib yam li phenolphthalein.
Dhau li ntawm, peb tuaj yeem siv nws ua cov cim xim los saib xyuas cov txheej txheem ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis. Cov tshuaj no tuaj yeem nqa tus nqi tsis zoo ntawm pH nruab nrab, qhov twg nws tuaj yeem txav mus rau tib qho kev coj ua DNA lossis cov protein nyob hauv gel. Tus nqi tsiv teb tsaws tuaj yeem sib txawv raws li gel ntom ntom thiab tsis muaj qhov sib xyaw. Txawm li cas los xij, nyob rau hauv ib txwm 1% agarose gel nyob rau hauv 1 lub sij hawm TAE tsis yog los yog TBE tsis, cov tshuaj no migrates nyob rau tib tus nqi raws li ib tug DNA seem ntawm txog 300 lub hauv paus khub.
Qhov txawv ntawm Xylene Cyanol thiab Bromophenol Blue yog dab tsi?
Xylene cyanol thiab bromophenol xiav yog cov cim xim tseem ceeb. Qhov sib txawv tseem ceeb ntawm xylene cyanol thiab bromophenol xiav yog tias nyob rau hauv 1% agarose gel, xylene cyanol migrates qeeb, whereas bromophenol xiav migrates sai.
Hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm xylene cyanol thiab bromophenol xiav hauv daim ntawv tabular rau kev sib piv ntawm ib sab.
Summary – Xylene Cyanol vs Bromophenol Blue
Xylene cyanol thiab bromophenol xiav yog qhov tseem ceeb raws li cov cim xim siv los saib xyuas cov txheej txheem ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis. Qhov sib txawv tseem ceeb ntawm xylene cyanol thiab bromophenol xiav yog tias nyob rau hauv 1% agarose gel, xylene cyanol migrates qeeb dua whereas bromophenol xiav migrates sai.
