Qhov sib txawv tseem ceeb ntawm AAV thiab lentivirus yog AAV muaj ib leeg-kab DNA genome thaum lentivirus muaj RNA genome.
Adeno-associated virus (AAV) yog ib tug kab mob DNA uas muaj ib leeg-kab DNA genome. Hauv qhov sib piv, lentiviruses yog cov kab mob RNA. Ob leeg AAV thiab Lentivirus yog cov tshuab xa cov noob tau zoo. Lawv zoo hloov cov noob rau hauv cov hlwb mammalian. Ntxiv mus, lawv muaj txiaj ntsig zoo hauv ntau daim ntawv thov, suav nrog cov noob qoob loo, kev qhia cov protein, thiab kev kho cov noob.
AAV is?
Adeno-associated virus (AAV) is a DNA virus. Nws muaj ib leeg-stranded DNA genome uas yog 4.8kb ua. Nws yog ib yam kab mob uas tsis yog-pob, kuj yooj yim thiab me me kab mob uas kis tau rau tib neeg thiab lwm yam primates, ua rau lub cev tsis muaj zog heev. Nws tsis ua rau mob. Txawm nyob hauv lub xeev hom tsiaj qus, AAV tsis yog kab mob rau tib neeg.
Figure 01: AAV
Zoo ib yam li Lentiviruses, AAV tuaj yeem tsim kho los siv los ua cov cuab yeej xa khoom muaj zog. Qhov tseeb, adeno-txuas kab mob vectors yog lub hauv paus rau kev kho noob ntawm tib neeg cov kab mob. Yog li ntawd, tus kab mob adeno-txuas txuas nrog recombinant yog ib qho kev cog lus zoo heev rau kev kho cov noob xa khoom vector.
Lentivirus yog dab tsi?
Lentivirus yog ib hom kab mob retroviruses. Lawv zwm rau tsev neeg ntawm retroviridae. Lentiviruses muaj xws li human immunodeficiency virus (HIV), simian immunodeficiency virus (SIV), feline immunodeficiency virus (FIV), thiab equine infectious anemia virus (EIAV). Lentiviruses muaj RNA genome. Yog li ntawd, lawv yog cov kab mob RNA. Lawv muaj cov enzyme reverse transcriptase. Cov enzyme no tuaj yeem hloov RNA rau hauv DNA ua ntej kev koom ua ke rau hauv tus tswv tsev genome.
Daim duab 02: Lentivirus
Lentiviruses tau siv dav ua cov vectors hauv kev xa DNA rau hauv cov hlwb mammalian. Lawv muaj ntau yam tseem ceeb uas muaj txiaj ntsig zoo hauv ntau qhov chaw, suav nrog kev coj noj coj ua ntawm tes, tsiaj qauv, thiab kev kho noob. Lawv muaj peev xwm kis tau yuav luag tag nrho cov hom ntawm mammalian hlwb. Lawv tuaj yeem siv los tsim cov kab ntawm tes ruaj khov lossis tsav cov kab mob ruaj khov hauv cov kabmob thiab cov ntaub so ntswg hauv vivo. Lentiviruses siv kev hloov pauv txhawm rau txhawm rau txhaj lawv cov genome rau hauv cov tswv tsev. Lawv tuaj yeem nqa cov ntxig lossis cov noob mus txog 8 kb loj.
Feem ntau, lentiviruses muaj kev nyab xeeb los daws. Lawv tsis hloov rov qab mus rau cov kab mob pathogenic. Txawm li cas los xij, muaj ntau qhov tsis zoo ntawm cov kab mob lentiviruses, xws li HIV keeb kwm thiab kev koom ua ke rau hauv tus tswv tsev genome, thiab lwm yam. Thaum lawv sib xyaw ua ke, nws tuaj yeem ua rau muaj kev hloov pauv hauv cov txheej txheem los ntawm kev cuam tshuam lwm cov noob tseem ceeb uas ua rau muaj txiaj ntsig loj. Li no, tus kheej-inactivating lentiviral vectors yog tsim los nrog kev cai ntawm kev koom ua ke rau hauv tus tswv tsev genome.
Dab tsi yog qhov zoo sib xws ntawm AAV thiab Lentivirus?
- AAV thiab lentivirus yog cov cuab yeej siv tau zoo rau kev hloov cov noob:
- Lawv ua haujlwm li vectors xa DNA rau hauv hlwb.
- Ob leeg siv kev hloov pauv los txhaj DNA rau hauv cov hlwb.
- Lawv yuav tsum tau tsim kho caj ces thiaj li siv tau hauv kev kho noob.
Qhov txawv ntawm AAV thiab Lentivirus yog dab tsi?
AAV yog tus kab mob DNA uas tsis muaj kab mob rau tib neeg, thaum lentivirus yog ib hom kab mob retroviruses uas yog kab mob rau tib neeg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm AAV thiab Lentivirus. Piv rau lentivirus, AAV kuj yooj yim thiab me me. Ntxiv mus, AAV tuaj yeem ua kom haum rau qhov ntxig ntawm 4.5 kb thaum lentiviruses tuaj yeem haum rau qhov ntxig ntawm 8 kb loj.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm AAV thiab lentivirus hauv daim ntawv teev npe rau kev sib piv ib sab.
Summary – AAV vs Lentivirus
AAV thiab lentivirus yog cov kab mob uas muaj txiaj ntsig zoo li cov tshuaj kho cov kab mob. AAV yog tus kab mob DNA uas tsis yog kab mob rau tib neeg. Nws yog lub luag haujlwm rau kev tiv thaiv kab mob me me, tsis zoo li lentivirus. Lentiviruses yog retroviruses uas ua rau cov kab mob tuag taus. Lawv yog cov kab mob RNA. HIV yog lentivirus. Thaum tsim kho, lentivirus ua raws li kev xa cov noob tau zoo. Yog li, qhov no qhia txog qhov sib txawv ntawm AAV thiab Lentivirus.
