Qhov sib txawv tseem ceeb ntawm p53 thiab p21 yog p53 ntes cov cell loj hlob los ntawm kev tuav lub voj voog ntawm tes ntawm G1 / S txoj cai taw tes thaum kuaj pom DNA puas, thaum p21 khi rau G1-S / CDK complexes thaum induction los ntawm p53 thiab inhibits cov haujlwm ntawm CDK complexes.
kho DNA yog txheej txheem los ntawm lub xovtooj sib koom txheeb xyuas thiab kho qhov kev puas tsuaj hauv DNA molecules. Ntau ntawm cov DNA no puas cuam tshuam rau DNA molecule. Qhov no tuaj yeem hloov pauv lub peev xwm ntawm tes los sau cov noob tseem ceeb, thaum kawg ua rau cov kab mob tuag taus xws li mob qog noj ntshav. DNA puas feem ntau ua rau p53-p21 txoj hauv kev thiab ua rau G1 theem ntes hauv cov hlwb.p53 thiab p21 yog ob qho tseem ceeb ntawm cov qog nqaij hlav proteins.
P53 yog dab tsi?
P53 yog cov qog nqaij hlav protein uas ntes cov cell loj hlob los ntawm kev tuav lub voj voog ntawm tes ntawm G1 / S txoj cai taw tes thaum kuaj pom DNA puas. Nws kuj yog tus saib xyuas ntawm genome. Homolog protein no tseem ceeb heev nyob rau hauv ntau cov kab mob vertebrates vim nws tiv thaiv kev tsim mob qog noj ntshav. Tsis tas li ntawd, P53 txuag kev ruaj ntseg ntawm cov genome los ntawm kev tiv thaiv kev hloov ntawm genome. Yog li ntawd, P53 yog suav hais tias yog cov qog nqaij hlav. Lub npe no tau pom thawj zaug hauv xyoo 1979 raws li cov molecular loj ntawm p53.
Daim duab 01: P53
Kev tshuaj xyuas SDS-PAGE qhia tias p53 yog 53 kilod alton (kDa) protein. Ntxiv mus, p53 noob yog cov noob feem ntau hloov pauv hauv tib neeg mob qog noj ntshav. Qhov no qhia txog lub luag haujlwm tseem ceeb ntawm p53 hauv kev tiv thaiv kab mob qog noj ntshav. P53 ua haujlwm ntau. Nws qhib DNA kho cov proteins, ntes cell loj hlob los ntawm kev tuav lub voj voog ntawm tes ntawm G1 / S txoj cai taw tes. Nws kuj tseem pib apoptosis thiab yog qhov tseem ceeb rau cov lus teb rau cov telomeres luv. Tsis tas li ntawd, induction ntawm p21 los ntawm p53 tom qab DNA kev puas tsuaj, uas inhibits Cdk4 thiab Cdk2 kev ua ub no, tuaj yeem ntes lub voj voog ntawm tes ntawm G / S txoj cai taw tes.
P21 yog dab tsi?
P21 yog qog nqaij hlav protein thiab CDK interacting protein uas muaj peev xwm khi rau G1-S / CDK complexes thaum induction los ntawm p53. Thaum khi, nws inhibits cov kev ua ntawm CDK complexes. Nws tseem hu ua cyclin-dependent kinase inhibitor. Qhov no yog vim nws muaj peev xwm ntawm inhibiting tag nrho cov cyclin / CDK complexes. Thaum induction los ntawm p53, p21 (CIPI / WAF1) khi thiab inhibits cyclin-CDK1, CDK2, CDK4/6 complexes. Yog li, nws ua haujlwm raws li tus tswj hwm ntawm lub voj voog ntawm tes ntawm G1 / S theem thaum DNA puas.
Daim duab 02: P21
Ntxiv mus, p21 cuam tshuam nrog proliferating cell nuclear antigen (PCNA) thiab inhibits nws. Nyob rau hauv no txoj kev, p21 ua raws li ib tug zoo inhibitor ntawm DNA synthesis nyob rau theem S. Txawm li cas los xij, nws tso cai kho nucleotide excision kho hauv S theem. Tsis tas li ntawd, cov protein no tau raug tshaj tawm tias tau cleaved los ntawm caspases zoo li CASP-3, uas ua rau muaj kev ua kom zoo ntawm CDK2 dua thiab yog li inhibits apoptosis.
Dab tsi zoo sib xws ntawm P53 thiab P21?
- P53 thiab P21 yog ob yam tseem ceeb ntawm qog nqaij hlav proteins.
- Ob leeg yog cov proteins me me uas hnyav hnyav.
- Cov proteins no koom nrog txoj hauv kev p53-p21.
- Ob leeg cov proteins tseem ceeb heev rau G1 theem ntes ntawm lub voj voog ntawm tes thaum lub sijhawm DNA puas.
- Ob leeg cov proteins muaj peev xwm tiv thaiv kev tsim mob qog noj ntshav.
Qhov txawv ntawm P53 thiab P21 yog dab tsi?
P53 yog cov qog nqaij hlav qog nqaij hlav uas ntes cov cell loj hlob los ntawm kev tuav lub voj voog ntawm tes ntawm G1 / S txoj cai taw qhia thaum kuaj pom DNA puas, thaum p21 yog qog nqaij hlav protein thiab CDK interacting protein uas muaj peev xwm los khi rau G1-S / CDK complexes thiab inhibiting kev ua ntawm CDK complexes raws li induction los ntawm p53. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm p53 thiab p21. Tsis tas li ntawd, P53 pib apoptosis yog tias DNA puas tsuaj tsis tuaj yeem hloov pauv tau tab sis, p21 inhibits apoptosis vim kev sib cais los ntawm caspases.
Cov hauv qab no infographic teev qhov sib txawv ntawm p53 thiab p21 hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – P53 vs P21
DNA kev puas tsuaj feem ntau ua rau p53-p21 txoj hauv kev thiab ua rau G1 theem ntes ntawm lub voj voog ntawm tes. Cov txheej txheem no tseem ceeb heev rau kev tswj cov qog nqaij hlav cancer. P53 thiab p21 yog ob qho tseem ceeb ntawm cov qog nqaij hlav qog nqaij hlav hauv txoj kev no. P53 yog cov protein uas ntes cov cell loj hlob los ntawm kev tuav lub voj voog ntawm tes ntawm G1 / S txoj cai taw tes thaum kuaj pom DNA puas, thaum p21 yog cov protein uas muaj peev xwm khi rau G1-S / CDK complexes thaum induction los ntawm p53 thiab inhibiting qhov kev ua haujlwm ntawm CDK complexes. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm p53 thiab p21.
