Qhov sib txawv tseem ceeb ntawm myristoylation thiab palmitoylation yog tias myristoylation yog txheej txheem rov qab tsis tau, qhov uas palmitoylation yog txheej txheem thim rov qab.
Myristoylation yog ib hom kev hloov kho lipid cov tshuaj tiv thaiv nyob rau hauv uas ib pawg myristoyl yog covalently ntxiv los ntawm amide daim ntawv cog lus rau alpha-amino pawg ntawm N-terminal glycine residue. Palmitoylation yog ib hom lipid hloov kho cov tshuaj tiv thaiv uas covalent bonding ntawm fatty acids rau cysteine tshwm sim.
Myristoylation yog dab tsi?
Myristoylation yog ib hom kev hloov kho lipid cov tshuaj tiv thaiv nyob rau hauv uas ib pawg myristoyl yog covalently ntxiv los ntawm amide daim ntawv cog lus rau alpha-amino pawg ntawm N-terminal glycine residue. Cov pab pawg myristoyl yog muab los ntawm myristic acid. Myristic acid yog saturated fatty acid uas muaj 14 carbon atoms ib molecule. Myristoylation yog ib qho lipidation, uas yog hom fatty acylation nyob rau hauv ntau yam kab mob xws li tsiaj, nroj tsuag, fungi, kab mob thiab protozoans. Cov tshuaj tiv thaiv no suav nrog kev tsis muaj zog ntawm cov protein-cov protein thiab cov protein-lipid kev sib cuam tshuam, uas muaj lub luag haujlwm tseem ceeb hauv kev tsom mus rau daim nyias nyias, ua haujlwm hauv cov teeb liab hloov txoj hauv kev, thiab lwm yam.
Daim duab 01: Mechanism of Myristoylation
Thaum xav txog cov txheej txheem ntawm Myristoylation cov tshuaj tiv thaiv, nws tshwm sim los ntawm nucleophilic ntxiv-kev tshem tawm cov tshuaj tiv thaiv. Cov txheej txheem tshuaj tiv thaiv no yog qhov tseem ceeb tsis yog hauv kev sib txawv ntawm kev ua haujlwm ntawm cov protein, tab sis kuj ntxiv cov txheej txheem kev tswj hwm rau cov protein. E.g. Cov txheej txheem no koom nrog hauv kev sib koom ua ke ntawm cov membrane thiab cellular localization ntawm cov protein hloov.
Palmitoylation yog dab tsi?
Palmitoylation yog ib hom kev hloov kho lipid cov tshuaj tiv thaiv uas covalent bonding ntawm fatty acids rau cysteine tshwm sim. Qhov loj fatty acid uas tau ntxiv zoo li no yog palmitic acid, thiab qhov sib ntxiv yuav tshwm sim tsis yog rau cysteine xwb tab sis kuj rau serine thiab threonine tsawg dua. Feem ntau, cov proteins no yog cov proteins membrane.
Daim duab 02: Cov txheej txheem biochemical cuam tshuam nrog Palmitoylation
Feem ntau, palmitoylation txhim kho cov hydrophobicity ntawm cov proteins thiab pab txhawb rau lub koom haum membrane. Nws kuj zoo nkaus li ua lub luag haujlwm tseem ceeb hauv kev lag luam subcellular ntawm cov proteins ntawm cov membrane compartments thiab hauv modulating protein-protein interactions. Cov txheej txheem tshuaj no yog cov txheej txheem dynamic. Nws tseem yog txheej txheem tom qab kev txhais lus.
Ntxiv mus, palmitoylation kho qhov kev sib raug zoo ntawm cov protein rau lipid rafts thiab pab txhawb kev sib koom ua ke ntawm cov protein. Qhov kev sib koom ua ke no tuaj yeem nce qhov sib thooj ntawm ob lub molecules. Tsis tas li ntawd, kev sib koom ua ke tuaj yeem tshem tawm cov protein kom deb ntawm lub substrate.
Qhov txawv ntawm Myristoylation thiab Palmitoylation yog dab tsi?
Myristoylation thiab Palmitoylation yog cov txheej txheem tshuaj lipid hloov kho. Myristoylation yog ib hom lipid hloov kho cov tshuaj tiv thaiv nyob rau hauv uas ib pab pawg neeg myristoyl yog covalently ntxiv rau alpha-amino pawg ntawm N-terminal glycine residue los ntawm amide daim ntawv cog lus, whereas palmitoylation yog ib hom lipid hloov kho cov tshuaj tiv thaiv uas fatty acids yog covalently bonded. rau cysteine. Qhov sib txawv tseem ceeb ntawm myristoylation thiab palmitoylation yog tias myristoylation yog ib qho kev hloov tsis tau, hos palmitoylation yog ib txoj kev thim rov qab. Tsis tas li ntawd, cov tshuaj tiv thaiv myristoylation cuam tshuam nrog kev tsis muaj zog ntawm cov protein-cov protein thiab cov protein-lipid kev sib cuam tshuam uas muaj lub luag haujlwm tseem ceeb hauv lub hom phiaj ntawm daim nyias nyias, ua haujlwm hauv cov teeb liab hloov txoj hauv kev, thiab lwm yam thaum palmitoylation txhim kho cov hydrophobicity ntawm cov protein thiab nws pab txhawb rau lub koom haum membrane.
Cov lus hauv qab no qhia txog qhov sib txawv ntawm myristoylation thiab palmitoylation.
Summary – Myristoylation vs Palmitoylation
Myristoylation thiab palmitoylation yog cov txheej txheem tshuaj lipid hloov kho. Qhov sib txawv tseem ceeb ntawm myristoylation thiab palmitoylation yog tias myristoylation yog ib qho kev hloov tsis tau, qhov uas palmitoylation yog cov txheej txheem thim rov qab.
