Qhov sib txawv tseem ceeb ntawm UV vis thiab fluorescence spectroscopy yog tias UV-pom spectroscopy ntsuas qhov nqus ntawm lub teeb nyob rau hauv UV-pom qhov ntau, whereas fluorescence spectroscopy ntsuas lub teeb tawm los ntawm cov qauv hauv cov fluorescence ntau tom qab nqus lub teeb ntawm lub zog siab dua li qib emitted zog.
Spectroscopy yog txheej txheem rau kev ntsuas qhov nqus thiab emission ntawm lub teeb thiab lwm yam hluav taws xob los ntawm teeb meem.
YUV Vis Spectroscopy yog dab tsi?
UV pom spectroscopy yog cov txheej txheem tshuaj xyuas uas siv qhov nqus lossis qhov cuam tshuam ntawm ib feem ntawm UV ntau yam thiab ua tiav cov cheeb tsam pom nyob ib sab ntawm electromagnetic spectrum. Cov txheej txheem no los ntawm ob hom; Lawv yog absorption spectroscopy thiab reflectance spectroscopy. Nws siv lub teeb nyob rau hauv qhov pom thiab nyob ib sab.
Feem ntau, qhov nqus lossis qhov cuam tshuam ntawm qhov pom ntawm lub teeb tuaj yeem cuam tshuam ncaj qha rau cov xim pom ntawm cov tshuaj uas koom nrog hauv cov txheej txheem. Ntawm qhov ntau ntawm cov spectrum no, peb tuaj yeem pom tias atoms thiab molecules tuaj yeem hloov pauv hluav taws xob. Ntawm no, kev nqus spectroscopy yog ntxiv rau fluorescence spectroscopy, qhov twg fluorescence cuam tshuam nrog kev hloov ntawm electrons los ntawm lub xeev zoo siab mus rau hauv av. Tsis tas li ntawd, kev nqus ntsuas qhov kev hloov pauv ntawm lub xeev hauv av mus rau lub xeev zoo siab.
Daim duab 01: UV pom Spectroscopy
Cov txheej txheem spectroscopic no muaj txiaj ntsig zoo hauv kev tshuaj xyuas cov qauv sib txawv ntawm ntau npaum li cas, xws li kev hloov pauv hlau ions, cov organic sib txuas ua ke, thiab macromolecules hauv cov kab mob lom. Feem ntau, kev soj ntsuam spectroscopic yog ua los ntawm kev siv cov kev daws teeb meem, tab sis peb kuj tuaj yeem siv cov khoom khib nyiab thiab cov pa roj.
Fluorescence Spectroscopy yog dab tsi?
Fluorescence spectroscopy yog hom electromagnetic spectroscopy uas muaj txiaj ntsig zoo hauv kev tshuaj xyuas fluorescence los ntawm cov qauv. Cov txheej txheem no suav nrog kev siv lub teeb ntawm lub teeb (xws li UV) los ua kom cov khoom siv hluav taws xob hauv cov molecules ntawm qee cov tebchaw, thiab nws tuaj yeem ua rau lawv tawm lub teeb. Feem ntau, qhov emission no pom lub teeb tab sis tsis tas yuav zoo ib yam.
Daim duab 02: Fluorescence Spectroscopy
Feem ntau, cov molecules muaj ntau lub xeev uas paub tias yog qib zog. Cov txheej txheem no feem ntau cuam tshuam nrog hluav taws xob thiab vibrational xeev. Feem ntau, cov qauv tshuaj ntsuam xyuas muaj cov molecules hauv av hluav taws xob hauv lub xeev thiab lub xeev zoo siab ntawm lub zog siab dua. Ob lub xeev hluav taws xob no muaj ntau lub xeev vibrational nruab nrab ntawm lawv. Thaum lub sij hawm tus txheej txheem ntawm fluorescence, cov tshuaj lom neeg zoo siab los ntawm absorbing photons thiab txav los ntawm ib tug hauv av lub xeev mus rau ib tug ntau dua lub zog. Tom qab ntawd, kev sib tsoo ntawm lwm cov molecules ua rau cov molecules zoo siab kom poob vibrational zog kom txog rau thaum nws los txog rau lub xeev tsis tshua muaj vibrational los ntawm lub xeev zoo siab no. Qhov no emits photons nrog ntau lub zog thiab ntau zaus. Qhov no hu ua fluorescence. Peb tuaj yeem txheeb xyuas cov zaus sib txawv no los txiav txim siab qhov sib txawv ntawm qhov sib txawv.
Qhov txawv ntawm UV Vis thiab Fluorescence Spectroscopy yog dab tsi?
Spectroscopic cov tswv yim muaj txiaj ntsig hauv kev kawm txog cov khoom ntawm cov tshuaj sib txawv. Qhov sib txawv tseem ceeb ntawm UV vis thiab fluorescence spectroscopy yog tias UV-pom spectroscopy ntsuas qhov nqus ntawm lub teeb nyob rau hauv UV-pom ntau yam, whereas fluorescence spectroscopy ntsuas lub teeb emission los ntawm ib tug qauv nyob rau hauv lub fluorescence ntau yam tom qab absorbing lub teeb ntawm siab zog tshaj lub emission. qib zog. Ntxiv mus, fluorescence spectroscopy yog rhiab tshaj li UV-pom spectroscopy.
Cov duab hauv qab no nthuav tawm qhov sib txawv ntawm UV vis thiab fluorescence spectroscopy hauv daim ntawv tabular rau kev sib piv ntawm ib sab.
Summary – UV Vis vs Fluorescence Spectroscopy
Spectroscopy yog ib qho tseem ceeb analytical txheej txheem. Muaj ntau hom spectroscopies, xws li IR spectroscopy, UV pom spectroscopy, fluorescence spectroscopy, thiab lwm yam. Qhov txawv tseem ceeb ntawm UV vis thiab fluorescence spectroscopy yog UV-pom spectroscopy ntsuas qhov nqus ntawm lub teeb nyob rau hauv UV-pom ntau yam, whereas fluorescence. spectroscopy ntsuas lub teeb emission los ntawm ib tug qauv nyob rau hauv lub fluorescence ntau yam tom qab absorbing lub teeb nyob rau hauv siab zog tshaj lub emitted zog theem.
