Qhov sib txawv tseem ceeb ntawm kev nce qib thiab rov ua dua staining yog tias nyob rau hauv kev loj hlob staining, cov ntaub so ntswg nyob rau hauv cov tshuaj staining tsuas yog ntev txaus kom ncav cuag qhov kawg xav tau thaum nyob rau hauv regressive staining, cov ntaub so ntswg yog txhob txwm tso tseg rau ntau staining kom txog thaum lub dye saturates tag nrho cov ntaub so ntswg thiab ces de-stained.
Staining yog ib qho txheej txheem uas ua kom pom tseeb thiab sib txawv ntawm cov ntaub so ntswg thiab ua rau nws muaj peev xwm soj ntsuam raws li lub tshuab tsom. H thiab E staining yog cov txheej txheem staining cov ntaub so ntswg uas feem ntau siv hauv histology. Nws siv hematoxylin thiab eosin (counterstain). Nuclear staining siv haematoxylin tuaj yeem ua tiav nrog cov txheej txheem staining zuj zus lossis rov ua dua. Qee cov tshuaj hematoxylin formulations yog siv nyob rau hauv staining zuj zus. Mayer's haematoxylin yog ib qho piv txwv. Progressive stains muaj qhov tsis tshua muaj hematoxylin concentration. Yog li, lawv maj mam thiab xaiv cov xim chromatin. Qee lwm cov formulations yog siv nyob rau hauv regressive staining. Harris haematoxylin yog siv nyob rau hauv regressive staining. Regressive stains muaj siab concentration ntawm hematoxylin; Yog li ntawd, cov stains sai heev diffuses tag nrho cov cell.
Kev Ua Txhaum Txhaum Cai yog dab tsi?
Txoj kev staining yog ib qho txheej txheem uas tso cai rau cov ntaub so ntswg hauv cov tshuaj staining ntev txaus kom ncav cuag qhov kawg xav tau. Yog li ntawd, yuav tsum tau saib xyuas qhov zoo ntawm cov stain zoo los txiav txim siab qhov ua tiav ntawm qhov staining. Lub staining siv yog tswj los ntawm lub sij hawm immersing.
Daim duab 01: Haematoxylin Structure
Gill's haematoxylin thiab Mayer's haematoxylin yog qhov muaj txiaj ntsig zoo. Rau ob lub hematoxylings no, staining lub sij hawm ntawm 5-10 feeb yog siv feem ntau nyob rau hauv staining zuj zus. Feem ntau, kev nce qib hematoxylins tsis tshua muaj siab. Yog li ntawd, lawv maj mam thiab xaiv stain chromatin. Hauv kev staining zuj zus, haematoxylin feem ntau stains cov chromatin rau qhov xav tau siv. Yog li, nws tsis tas yuav muaj kev sib txawv hauv cov kua qaub kua qaub kom tshem tawm cov kab ntau dhau.
Regressive Staining yog dab tsi?
Regressive staining yog txheej txheem staining sai dua uas cov ntaub so ntswg txhob txwm tshaj tawm kom txog thaum cov xim saturates tag nrho cov ntaub so ntswg. Tom qab ntawd cov ntaub so ntswg yog xaiv de-stained kom txog thaum nws mus txog qhov tseeb kawg. De-staining kauj ruam yog hu ua kev sib txawv. Kev sib txawv yog ua kom tshem tawm cov stains ntau dhau. Feem ntau, nws yog ua tiav siv dilute acid cawv.
Daim duab 02: H thiab E Staining
Harris haematoxylin yog hom hematoxylin nrov. Ehrlich's thiab Delafield's haematoxylins kuj tseem siv rau hauv kev ua kom tsis zoo. Regressive stains muaj siab concentration ntawm hematoxylin. Yog li ntawd, lawv sai sai diffuse tag nrho cov xov tooj ntawm tes thiab dhau ntawm cov chromatin thiab cytoplasm. Regressive staining yog qhov zoo dua thaum xav tau qhov sib txawv ntawm cov ntaub so ntswg.
Dab tsi yog qhov zoo sib xws ntawm Kev Tshaj Lij Tshaj Lij Tshaj Lij Tshaj Lij Tshaj Lij Tshaj Lij Tshaj Tawm?
- Txoj kev staining thiab regressive staining yog ob hom kev staining.
- Ob leeg siv cov xim hu ua haematoxylin.
Dab tsi yog qhov sib txawv ntawm Kev Txhim Kho thiab Kev Ua Kom Zoo Dua?
Txoj kev staining yog ib txoj kev staining qeeb qeeb uas cov ntaub so ntswg tso rau hauv cov tshuaj staining ntev txaus kom ncav cuag qhov kawg xav tau. Nyob rau hauv sib piv, regressive staining yog ib tug sai staining txheej txheem nyob rau hauv uas cov ntaub so ntswg yog txhob txwm tshaj stained thiab ces de-stained. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev nce qib thiab rov ua dua staining.
Ntxiv mus, lwm qhov sib txawv tseem ceeb ntawm kev nce qib thiab rov ua dua staining yog qhov kev staining zuj zus tsis tas yuav tsum muaj cov kauj ruam ntxiv hu ua kev sib txawv, thaum cov staining regressive yuav tsum muaj kev sib txawv txhawm rau tshem tawm cov stains ntau dhau. Tsis tas li ntawd, cov stains zuj zus muaj qhov tsis tshua muaj siab ntawm haematoxylins, thaum cov stains regressive muaj siab dua ntawm haematoxylins.
Cov hauv qab no infographic tabulates ntau qhov sib txawv ntawm kev nce qib thiab rov ua dua staining.
Summary – Progressive vs Regressive Staining
Txoj kev staining yog cov txheej txheem staining qeeb uas cov ntaub so ntswg tso rau hauv cov tshuaj staining ntev txaus kom ncav cuag qhov kawg. Yog li ntawd, over staining tsis ua tiav yog kev staining. Nyob rau hauv sib piv, regressive staining yog ib tug ceev staining txheej txheem nyob rau hauv uas cov ntaub so ntswg tshaj stained thiab ces de-stained. Progressive staining tsis tas yuav tsum muaj kev sib txawv (tshem tawm cov stain ntau dhau) thaum regressive staining yuav tsum muaj kev sib txawv hauv cov kua qaub cawv kom tshem tawm cov stain ntau dhau. Progressive stains feem ntau muaj qhov tsis tshua muaj siab ntawm haematoxylins thaum cov stains regressive muaj ntau dua ntawm haematoxylins. Yog li ntawd, qhov no qhia txog qhov sib txawv ntawm kev loj hlob thiab regressive staining.
