Qhov sib txawv tseem ceeb ntawm excitation thiab emission lim yog qhov excitation lim yog qhov tseem ceeb hauv kev ua kom pom tseeb ntawm cov khoom nyob rau hauv kev tshuaj xyuas ntawm lub tshuab ntsuas qhov ntsuas emission tseem ceeb hauv kev ua kom cov khoom nyob ib puag ncig ntawm qhov tsaus ntuj li sai tau.
Cov ntsiab lus excitation thiab emission lim yog siv feem ntau hais txog fluorescence microscopy uas ua haujlwm raws li cov lim dej kho qhov muag. Muaj peb yam khoom siv hauv cov cuab yeej fluorescence microscopic: excitation filter, dichroic beamsplitter, thiab emission filter.
Dab tsi yog Excitation Filter?
Ib lub lim dej yog ib hom kev kho qhov muag iav lim uas muaj txiaj ntsig zoo hauv kev xaiv lub zog ntawm lub teeb. Feem ntau, nws yog qhov zoo tshaj plaws kho qhov muag iav dav siv hauv fluorescence microscopy thiab spectroscopic daim ntaub ntawv uas peb yuav tsum xaiv lub wavelength los ntawm lub teeb teeb uas los ntawm lub teeb ci.
Feem ntau, cov ntxaij lim dej zoo siab xaiv lub teeb uas muaj qhov luv luv uas los ntawm lub teeb pom kev zoo. Qhov no yog vim hais tias lub teeb hom no muaj peev xwm nqa tau lub zog uas txaus tsuas yog rau cov fluoresce ntawm ib yam khoom uas tau soj ntsuam nyob rau hauv lub microscope.
Daim duab 01: Kev kuaj xyuas hauv lub tshuab ntsuas qhov ntsuas pom ntawm fluorescence
Muaj ob hom kev ua kom pom lub ntsej muag zoo li luv hla lub tsom iav thiab bandpass lim tsom iav. Ob hom lim tsom iav no txawv ntawm ib leeg raws li daim ntawv ntawm cov ntxaij lim dej lossis cov lim dej sib sib zog nqus uas siv los ua cov tsom iav emission. Txawm li cas los xij, tuaj yeem muaj qee yam ntawm cov iav lim dej zoo ib yam, xws li monochromators, cov kab nrib pleb uas ua ke nrog qhov nqaim, thiab holographic diffraction gratings.
Feem ntau, lub iav lim dej los hauv ib pob nrog cov lim dej tawm thiab cov kab hluav taws xob dichroic splitter hauv lub voos xwmfab. Yog li ntawd, peb tuaj yeem ntxig ob lub iav no ua ke ua ke rau hauv lub tshuab tsom. Lub dichroic beam nyiam tswj lub wavelength ntawm lub teeb uas nkag mus rau txhua lub iav lim.
Emission Filter yog dab tsi?
Ib lub emission lim yog ib hom iav kho qhov muag uas tso cai rau lub wavelengths emissions los ntawm fluorophore mus hla nws. Cov iav kho qhov muag no yog lub npe hu ua emitter lossis barrier lim ib yam. Nws yog lub npe hu ua barrier lim vim tias nws tuaj yeem thaiv tag nrho cov teeb pom kev tsis zoo sab nraud ntawm lub zog excitation uas los ntawm lub teeb excitation. Qhov kev lim dej thaiv no tso cai rau keeb kwm yav dhau los ntawm cov khoom uas tab tom kuaj xyuas los ntawm lub tshuab tsom iav kom qhov tsaus ntuj tshaj plaws li qhov ua tau.
Feem ntau, lub iav lim dej tawm los hauv ib pob nrog lub lim dej kom zoo thiab lub teeb dichroic beam splitter hauv ib lub voos xwmfab. Yog li ntawd, peb tuaj yeem ntxig ob lub iav no ua ke ua ke rau hauv lub tshuab tsom. Nyob ntawd, lub teeb dichroic nyiam tswj lub wavelength ntawm lub teeb uas nkag mus rau txhua lub iav lim.
Qhov txawv ntawm Excitation thiab Emission Lim yog dab tsi?
Nws muaj peb yam khoom siv hauv cov cuab yeej fluorescence microscopic uas suav nrog cov lim dej, dichroic beamsplitter, thiab lim emission. Qhov sib txawv tseem ceeb ntawm excitation thiab emission lim yog qhov excitation lim yog qhov tseem ceeb hauv kev ua kom pom tseeb ntawm cov khoom nyob rau hauv kev tshuaj ntsuam xyuas ntawm lub tshuab tsom iav qhov emission lim yog qhov tseem ceeb hauv kev ua kom cov khoom nyob ib puag ncig tsaus ntuj li sai tau.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm kev ua kom zoo siab thiab lim dej tawm hauv daim ntawv teev npe.
Summary – Excitation vs Emission Filter
Qhov sib txawv tseem ceeb ntawm excitation thiab emission lim yog qhov excitation lim yog qhov tseem ceeb hauv kev ua kom pom tseeb ntawm cov khoom nyob rau hauv kev tshuaj xyuas ntawm lub tshuab ntsuas qhov ntsuas emission tseem ceeb hauv kev ua kom cov khoom nyob ib puag ncig ntawm qhov tsaus ntuj li sai tau.
