Qhov sib txawv tseem ceeb ntawm PFGE thiab CRP tsom xam yog tias PFGE yog cov txheej txheem kuaj siv rau kev sib cais ntawm DNA loj molecules los ntawm kev siv lub tshuab hluav taws xob uas hloov pauv raws sijhawm, thaum CRP (c-reactive protein) tsom xam yog cov txheej txheem uas kuaj pom lub nplhaib zoo li cov protein hauv cov ntshav plasma.
PFGE thiab CRP tsom xam yog ob txoj kev siv los ntawm cov kws tshawb fawb los tshawb xyuas cov kab mob sib txawv uas ua rau muaj kev puas tsuaj rau tib neeg lub cev. Pulsed-field gel electrophoresis (PFGE) yog cov txheej txheem kuaj ncaj qha uas kuaj pom DNA ntiv tes ntawm tus kab mob, tshwj xeeb tshaj yog cov kab mob cais tawm. CRP, ntawm qhov tod tes, yog ib qho kev kuaj tsis ncaj uas siv los txheeb xyuas cov protein tshwj xeeb hauv cov ntshav uas tshwm sim los ntawm qhov mob. Cov kab mob no feem ntau yog kis kab mob los yog lwm yam kab mob.
PFGE yog dab tsi?
Pulsed-field gel electrophoresis yog cov txheej txheem siv rau kev sib cais ntawm DNA loj molecules hauv gel matrix los ntawm kev siv hluav taws xob. Lub tshuab hluav taws xob tsis tu ncua hloov nws cov kev taw qhia. Nws yuav siv tau rau genetic engineering lossis genotyping ntawm cov kab mob sib txawv. Cov txheej txheem rau PFGE kuj zoo ib yam li cov txheej txheem gel electrophoresis. Qhov txawv tsuas yog qhov hluav taws xob uas hloov pauv ntawm peb cov lus qhia ib ntus. Ib qho kev taw qhia khiav los ntawm lub hauv paus axis ntawm gel thaum lwm tus khiav ntawm lub kaum sab xis ntawm 60 degrees ntawm ob sab. Raws li lub sij hawm mem tes sib npaug rau txhua qhov kev taw qhia, nws ua rau kev txav mus los ntawm DNA.
Daim duab 01: PFGE Analysis
Cov txheej txheem no siv sijhawm ntev dua li cov gel electrophoresis dav dav vim qhov loj ntawm cov khoom tawg tau raug daws. Nyob rau hauv cov txheej txheem PFGE, nrog rau kev hloov pauv ntawm hluav taws xob kev taw qhia, ntau qhov ntev ntawm DNA hnov mob rau cov kev hloov pauv ntawm qhov sib txawv. Cov DNA loj loj rov ua kom qeeb qeeb hauv kev sib piv rau cov DNA me me uas rov zoo sai. Yog li ntawd, kev sib cais zoo dua ntawm DNA tawg yog ua tau los ntawm cov txheej txheem no.
PFGE feem ntau yog siv los kuaj cov kab mob xws li Listeria monocytogenes. Nws yog ib txoj hauv kev zoo los kuaj xyuas kab mob.
CRP Analysis yog dab tsi?
C-reactive protein (CRP) yog ib qho annular (ring-shaped) pentameric protein pom nyob rau hauv cov ntshav plasma. Feem ntau, CRP nce nyob rau hauv cov ntsiab lus teb rau o. Kev tshuaj xyuas CRP yog ua raws li kev tshawb pom ntawm cov protein no hauv cov ntshav plasma. CRP protein yog synthesized los ntawm lub siab nyob rau hauv teb rau tej yam tseem ceeb secreted los ntawm macrophages thiab fats hlwb. CRP protein yog thawj tus qauv lees paub receptor (PRR) kom raug txheeb xyuas.
Daim duab 02: CRP Analysis
nce CRP protein ntau tuaj yeem pom tom qab raug mob, mob plawv, mob autoimmune, thiab kab mob xws li kab mob sepsis. Qhov ib txwm muaj CRP protein ntau hauv cov ntshav plasma yog 0.3-10 milligram ib liter (mg / L). Cov ntshav yog kos los ntawm cov hlab ntsha, feem ntau yog los ntawm caj npab ntawm tus neeg mob. Kev tsom xam yog ua los ntawm kev siv cov txheej txheem ntsuas biochemical. Hauv kev kuaj CRP, CRP qib siab dua 10 mg / L yog qhov qhia tau tias muaj kab mob hnyav (sepsis). CRP test tsis piav qhia qhov chaw lossis qhov ua rau mob. Yog li ntawd, cov kws kho mob yuav tsum tau pom zoo kom kuaj ntxiv los piav qhia qhov ua rau mob. Qee zaum, CRP protein ntau nce vim haus luam yeeb, rog rog, thiab tsis muaj kev tawm dag zog ib yam nkaus.
Dab tsi zoo sib xws ntawm PFGE thiab CRP Kev Ntsuam Xyuas?
- PFGE thiab CRP tsom xam yog cov txheej txheem kev sim siv hauv chav kuaj.
- Ob qho kev sim tsom biomolecules.
- Lawv siv los kuaj tib neeg cov kab mob.
- Cov kev sim no muaj peev xwm kuaj tau cov kab mob.
Qhov txawv ntawm PFGE thiab CRP Analysis yog dab tsi?
PFGE yog cov txheej txheem kuaj siv rau kev sib cais ntawm DNA loj molecules los ntawm kev siv lub tshuab hluav taws xob uas hloov pauv raws sijhawm. Hauv qhov sib piv, CRP tsom xam yog ib qho txheej txheem uas kuaj pom lub nplhaib-zoo li cov proteins hauv cov ntshav plasma. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm PFGE thiab CRP tsom xam. Ntxiv mus, PFGE pom cov kab mob ncaj qha, thaum CRP tsom xam pom cov kab mob tsis ncaj.
Hauv qab no yog cov npe ntawm qhov sib txawv tseem ceeb ntawm PFGE thiab CRP tsom xam hauv daim ntawv teev npe.
Summary – PFGE vs CRP Analysis
PFGE thiab CRP tsom xam yog ob qhov kev ntsuam xyuas niaj hnub siv hauv cov chaw kuaj mob. PFGE yog cov txheej txheem kuaj siv los ntawm cov kws tshawb fawb los tsim DNA ntiv tes, tshwj xeeb tshaj yog cov kab mob kab mob. Kev kuaj C-reactive protein (CRP) yog siv los kuaj mob los ntawm kev kuaj pom c-reactive protein nyob rau hauv cov ntshav plasma. Ob qhov kev ntsuam xyuas tseem ceeb hauv kev kuaj xyuas tib neeg cov kab mob. Yog li, qhov no qhia txog qhov sib txawv ntawm PFGE thiab CRP tsom xam.
