Qhov sib txawv tseem ceeb ntawm hematoxylin thiab eosin yog hematoxylin yog ib qho yooj yim dye, whereas eosin yog acidic dye.
Hematoxylin thiab eosin yog qhov tseem ceeb hauv histology rau staining lub hom phiaj. Ob lub dyes no yog siv los ua counterstains hauv H thiab E staining txheej txheem.
Hematoxylin yog dab tsi?
Hematoxylin yog cov organic compound uas muaj cov tshuaj formula C16H14O6 Qhov no compound yog muab rho tawm los ntawm heartwood ntawm tsob ntoo logwood. Qhov no yog ib tug natural dye uas tau ntev siv los ua ib tug histologic stain, number case thiab raws li ib tug dye nyob rau hauv lub textile thiab tawv kev lag luam. Raws li kev zas xim, cov khoom siv no muaj ntau lub npe xws li Palo De Campeche, longwood extract, bluewood thiab blackwood.
Feem ntau, hauv kev siv histological, hematoxylin stain yog ua ke nrog eosin los ntawm H thiab E staining txheej txheem, thiab nws yog ib qho kev sib xyaw ua ke hauv daim teb ntawm histology. Ntxiv mus, hematoxylin yog ib feem ntawm Papanicolaou stain, uas muaj ntau yam siv hauv kev kawm cytology specimens.
Daim duab 01: Cov qauv ntawm Hematoxylin
Cov xim nquag ntawm hematoxylin yog haematin, daim ntawv oxidized uas tuaj yeem tsim cov xim zoo nkauj uas muaj cov hlau ions xws li Fe(III) thiab Al(III) ntsev. Thaum hematoxylin ntshiab, nws zoo nkaus li cov xim tsis muaj kob crystalline khoom uas muaj feem ntau lub teeb rau tsaus xim av tsos hauv qib lag luam vim muaj impurities.
Kev txiav txim siab txog kev rho tawm thiab kev ua kom huv ntawm hematoxylin dye, nws ua tiav los ntawm kev ua kom cov ntoo chips hauv Fabkis txoj txheej txheem lossis American txheej txheem (uas suav nrog chav thiab siab). Cov xim no yog muag raws li cov kua concentrated, cov khoom qhuav, los yog cov khoom siv crystalline ib zaug muab rho tawm. Txawm li cas los xij, muaj qee qhov kev tsim khoom niaj hnub no, suav nrog kev siv dej, ether, lossis cawv ua cov kuab tshuaj.
Eosin yog dab tsi?
Eosin yog ib pawg ntawm cov tshuaj fluorescent acidic uas muaj cov ntawv cog lus nrog cov ntsiab lus yooj yim lossis eosinophilic tebchaw los tsim cov ntsev. Cov ntsev yog tsim nrog cov tebchaw xws li cov proteins uas muaj cov amino acid residue nrog rau arginine, thiab lysine. Eosin tuaj yeem stain protein residues tsaus liab lossis liab xim vim qhov kev ua ntawm bromine ntawm fluorescein. Tsis tas li ntawd, peb tuaj yeem siv cov xim no los stain cov proteins hauv cytoplasm thiab ua kom cov collagen thiab cov nqaij fibers rau kev kawm hauv lub tshuab tsom. Hauv cov ntsiab lus no, peb tuaj yeem ua lub npe microstructures uas yooj yim stain nrog eosin raws li cov qauv eosinophilic.
Daim duab 02: Tus qauv ntawm Eosin Y
Thaum xav txog ntau hom eosin, muaj ob qhov sib txuas zoo sib xws ntawm eosin li eosin Y thiab eosin B. Ntawm lawv, Eosin Y muaj lub ntsej muag daj me ntsis, thaum Eosin B muaj lub ntsej muag daj daj. Peb tuaj yeem siv cov dyes sib hloov.
Muaj ntau daim ntawv thov ntawm eosin hauv histology los ua cov tshuaj tiv thaiv rau haematoxylin hauv H thiab E staining. Nws yog cov txheej txheem ntau tshaj plaws hauv histology. Thaum lub cytoplasm yog staining siv H thiab E staining, peb tuaj yeem soj ntsuam nws cov xim hloov raws li cov xim liab-txiv kab ntxwv thiab nuclei stained nyob rau hauv cov xim tsaus nti hauv xiav lossis liab doog xim.
Qhov txawv ntawm Hematoxylin thiab Eosin yog dab tsi?
Hematoxylin thiab eosin yog cov tshuaj pleev xim tseem ceeb hauv histology. Cov no yog cov khoom counterstain rau H thiab E staining txheej txheem. Hematoxylin yog ib qho organic compound uas muaj cov tshuaj formula C16H14O6 thaum eosin yog ib pawg ntawm fluorescent acidic tebchaw muaj kev sib txuas nrog cov ntsiab lus los yog eosinophilic tebchaw los tsim cov ntsev. Qhov sib txawv tseem ceeb ntawm hematoxylin thiab eosin yog hematoxylin yog cov xim yooj yim, qhov eosin yog cov kua qaub dye.
Hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm hematoxylin thiab eosin hauv daim ntawv teev npe.
Summary – Hematoxylin vs Eosin
Hematoxylin thiab eosin yog cov tshuaj zas xim tseem ceeb hauv cov txheej txheem staining xws li cov protein hauv cytoplasm. Qhov sib txawv tseem ceeb ntawm hematoxylin thiab eosin yog hematoxylin yog cov xim yooj yim, qhov eosin yog cov kua qaub dye.
