Qhov txawv ntawm Harris thiab Mayer's Haematoxylin

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Qhov txawv ntawm Harris thiab Mayer's Haematoxylin
Qhov txawv ntawm Harris thiab Mayer's Haematoxylin

Video: Qhov txawv ntawm Harris thiab Mayer's Haematoxylin

Video: Qhov txawv ntawm Harris thiab Mayer's Haematoxylin
Video: Qhov xaus ntawm tus poj niam deev hluas 2/20/2021 2024, Kaum ib hlis
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Qhov sib txawv tseem ceeb ntawm Harris thiab Mayer's haematoxylin yog Harris haematoxylin yog siv rau hauv kev ua kom tsis zoo thaum Mayer's haematoxylin yog siv rau hauv kev staining.

Haematoxylin yog ib qho yooj yim zas xim uas feem ntau siv hauv histopathology. Nws yog cov xim ntuj tso tawm los ntawm tsob ntoo Haematoxylon campechianum. Nws yog xim xiav tsaus los yog liab doog xim dye. Haematoxylin tso cai rau kev ua qauv qhia ntawm microscopic nuclear cov ntsiab lus ntawm cov cellular thiab cov ntaub so ntswg. Cov dye no tau them zoo, yog li ntawd, khi nrog cov khoom tsis zoo xws li DNA thiab RNA thiab ua rau lawv muaj xim violet. Haematoxylin yuav tsum muaj mordant los pab kev sib txuas nrog cov ntaub so ntswg. Cov mordant yog cov tshuaj, tshwj xeeb tshaj yog cov ntsev ntawm txhuas, hlau, tungsten, uas pab txhawb kev sib txuas ntawm cov xim rau cov ntaub so ntswg.

Raws li mordants lawv muaj, muaj ntau hom hematoxylins muaj. Qee tus ntawm lawv yog Ehrlich's, Mayer's, Harris', Gill's, Delafield's, Cole's thiab Carazzi's haematoxylins. Ob leeg Harris 'thiab Mayer's haemoxylin formulations yog txhuas-raws li mordant haematoxylins.

Harris Haematoxylin yog dab tsi?

Harris haematoxylin yog cov xim yooj yim uas feem ntau siv regressively hauv histology labs rau niaj hnub H thiab E staining. Harris haematoxylin siv txhuas raws li nws cov mordant txhawm rau txuas nrog cov ntaub so ntswg. Thaum lub sij hawm regressive staining, cov ntaub so ntswg yog over-stained nrog Harris haematoxylin. Yog li ntawd, nws yuav tsum tau sib txawv nrog dilute acid cawv.

Qhov txawv tseem ceeb - Harris vs Mayer's Haematoxylin
Qhov txawv tseem ceeb - Harris vs Mayer's Haematoxylin

Daim duab 01: Haematoxylin Powder

Harris haematoxylin muaj cov ntsiab lus siab ntawm hematoxylin. Yog li ntawd, nws sai heev diffuses tag nrho cov cell. Harris haematoxylin stains lub nuclei hauv maub violet-xiav.

Mayer's Haematoxylin yog dab tsi?

Mayer's haematoxylin yog cov xim yooj yim siv hauv kev ua staining. Nws yuav siv sij hawm 5 mus rau 10 feeb rau staining. Zoo ib yam li Harris haematoxylin, Mayer's haematoxylin yog ib qho txhuas-raws li mordant haematoxylin. Yog li ntawd, nws siv txhuas raws li nws cov mordant thiaj li yuav txuas nrog cov ntaub so ntswg tivthaiv.

Qhov txawv ntawm Harris thiab Mayer's Haematoxylin
Qhov txawv ntawm Harris thiab Mayer's Haematoxylin

Daim duab 02: Haematoxylin

Mayer's haematoxylin stain muaj qhov tsis tshua muaj siab ntawm hematoxylin. Yog li ntawd, nws maj mam thiab xaiv stains chromatin. Nws tsis tas yuav muaj kev sib txawv kom tshem tawm cov stain ntau dhau. Ntxiv mus, Mayer cov kev daws teeb meem haematoxylin yog qhov ua tau zoo ntawm kev hloov pauv me me hauv lub sijhawm thov.

Dab tsi yog qhov Zoo sib xws ntawm Harris thiab Mayer's Haematoxylin?

  • Harris thiab Mayer's Haematoxylin yog ob hom haematoxylins cais raws li mordant lawv muaj.
  • Lawv yog cov xim ntuj muab rho tawm los ntawm cov nroj tsuag.
  • Ob leeg yog aluminium-based mordant haematoxylins.
  • Qhov tseeb, lawv yog nuclear stains.
  • Lawv xim nuclei xiav.
  • Lawv feem ntau yog siv rau niaj hnub staining hauv histology thiab histopathology.
  • lawv ruaj khov siab.
  • Cov txhuas raws li haematoxylins yog qhov ua tau zoo dua rau kev siv txuas ntxiv.

Qhov txawv ntawm Harris thiab Mayer's Haematoxylin yog dab tsi?

Qhov sib txawv tseem ceeb ntawm Harris thiab Mayer's haematoxylin yog lawv siv. Harris haematoxylin yog siv nyob rau hauv regressive staining thaum Mayer's haematoxylin yog siv nyob rau hauv staining zuj zus. Harris haematoxylin muaj cov ntsiab lus siab ntawm hematoxylin. Yog li ntawd, nws sai heev diffuses tag nrho cov cell. Mayer's haematoxylin stain, ntawm qhov tod tes, muaj qhov tsis tshua muaj siab ntawm hematoxylin. Li no, nws maj mam thiab xaiv stains chromatin.

Hauv qab infographic tabulates ntau qhov sib txawv ntawm Harris thiab Mayer's haematoxylin.

Qhov txawv ntawm Harris thiab Mayer's Haematoxylin hauv Tabular Form
Qhov txawv ntawm Harris thiab Mayer's Haematoxylin hauv Tabular Form

Summary – Harris vs Mayer's Haematoxylin

Haematoxylin yog qhov nrov tshaj plaws siv dye hauv histology. Yog li ntawd, qhov stain no feem ntau siv rau niaj hnub kuaj histological ntawm cov ntaub so ntswg. Harris haematoxylin thiab Mayer's haematoxylin yog ob qho tshuaj alum haematoxylin. Ob leeg stain nuclei hauv cov xim xiav tsaus. Harris haematoxylin yog siv nyob rau hauv regressive staining thaum Mayer's haematoxylin yog siv nyob rau hauv staining zuj zus. Harris haematoxylin sai dhau-stains cov ntaub so ntswg, yog li xav tau kev sib txawv kom tshem tawm cov stain ntau dhau. Mayer's haemoxylin tsis dhau-stains cov ntaub so ntswg. Yog li, qhov no qhia txog qhov sib txawv ntawm Harris thiab Mayer's haematoxylin.

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