Qhov sib txawv tseem ceeb ntawm Elisa thiab sab hnub poob blot yog Elisa lossis enzyme-txuas immunoassay yog cov cuab yeej kuaj mob uas kuaj pom tias tus neeg mob puas tau kis tus kab mob tshwj xeeb lossis lwm tus kab mob kis tau thaum western blot yog cov txheej txheem uas kuaj pom cov protein tshwj xeeb los ntawm cov qauv protein.
AIDS tau dhau los ua teeb meem thoob ntiaj teb, thiab qhov xwm txheej ntawm tus kab mob tuag taus no tau nce siab txaus ntshai nyob rau ob peb xyoos dhau los. Kev kuaj kab mob HIV kuaj pom muaj tus kab mob tiv thaiv kab mob tib neeg lub cev tiv thaiv kab mob (HIV) uas ua rau muaj tus kab mob no. Tawm ntawm cov kev sim HIV sib txawv, ELISA thiab Western Blot yog nrov heev niaj hnub no.. Nws yog ib qho enzyme immunoassay siv los kuaj cov kab mob HIV. Western blot kuaj pom cov protein tshwj xeeb los ntawm cov protein sib xyaw los ntawm kev siv cov tshuaj tiv thaiv kab mob.
Key Difference
1. Txheej txheem cej luam thiab Qhov Tseem Ceeb Qhov txawv
2. Elisa yog dab tsi
3. Western Blot yog dab tsi
4. Zoo sib xws Ntawm Elisa thiab Western Blot
5. Ib sab ntawm Kev Sib Piv - Elisa vs Western Blot hauv Daim Ntawv Qhia
6. Summary
Elisa yog dab tsi?
ELISA, uas sawv cev rau enzyme-linked immunosorbent assay, yog thawj qhov kev sim tsim los rau kev kuaj pom HIV. Nws yog ib qho kev ntsuam xyuas phaj. Nws yog siv los kuaj xyuas cov tshuaj tiv thaiv kab mob HIV, thiab qhov kev sim no qhia tau tias muaj kev nkag siab zoo. Nyob rau hauv cov txheej txheem no, cov antigens nyob rau hauv cov qauv khi nrog cov immobilized antibodies ntawm ib tug khoom nto. Thaum immobilized, cov antigens tsim complexes nrog cov tshuaj tiv thaiv kab mob txuas rau enzymes. Tom qab ntawd cov enzymes conjugated incubate nrog cov substrate.
Daim duab 01: ELISA
Enzyme-substrate cov tshuaj tiv thaiv tsim cov khoom xim uas ntsuas tau. Los ntawm kev ntsuas tus nqi ntawm cov khoom, tus naj npawb ntawm cov antigens nyob rau hauv cov qauv tuaj yeem suav tau. Yog li, ELISA yog cov txheej txheem tshwj xeeb thiab cov txheej txheem uas xav tau kev saib xyuas ntawm cov kws tshaj lij. Nws tseem yog cov txheej txheem siv sijhawm nrog cov khoom siv siab heev.
Western Blot yog dab tsi?
Western blotting yog cov txheej txheem uas ua kom pom cov protein tshwj xeeb los ntawm cov protein sib xyaw. Western blot yog daim nyias nyias siv thaum lub sijhawm blotting kom tau txais daim iav duab ntawm cov qauv protein hauv SDS-polyacrylamide gel. Lub membrane siv rau sab hnub poob blotting feem ntau yog ua los ntawm nitrocellulose los yog polyvinylidene difluoride (PVDF). Cov membrane nrog cov protein hloov pauv tuaj yeem siv los txheeb xyuas cov protein tshwj xeeb. Yuav tsum muaj cov tshuaj tiv thaiv zoo rau kev kuaj pom cov protein uas xav tau los ntawm hybridization. Cov tshuaj tiv thaiv kab mob khi nrog nws cov tshuaj tiv thaiv tshwj xeeb thiab qhia pom muaj qhov xav tau antigen, uas yog cov protein.
Daim duab 02: Western Blot
Kev xa cov proteins los ntawm SDS polyacrylamide gel mus rau sab hnub poob blot yog ua los ntawm electroblotting. Nws yog ib txoj hauv kev zoo thiab ceev ceev uas ua rau cov proteins kom electrophorese tawm ntawm cov gel thiab dhau mus rau nitrocellulose membrane (western blot).
Western blotting yog qhov tseem ceeb hauv kev tshawb nrhiav cov tshuaj tiv thaiv kab mob HIV hauv tib neeg cov ntshav ntshav. Western blot kuj tseem siv tau los ua qhov kev kuaj pom tseeb rau tus kab mob siab B thiab kuaj pom tseeb rau tus kab mob nyuj vwm.
Dab tsi zoo sib xws ntawm Elisa thiab Western Blot?
- Elisa thiab western blot yog ob hom kev siv los kuaj kab mob HIV.
- Ob txoj kev yog ua raws li kev tiv thaiv kab mob.
- Lawv yog raws li kev tsim cov tshuaj tiv thaiv-protein complex.
- Ob txoj kev tuaj yeem txheeb xyuas cov proteins.
- Elisa thiab western blotting yog cov tswv yim siv sijhawm.
- Cov neeg ua haujlwm tau txais kev cob qhia zoo thiab cov neeg txawj ntse yuav tsum tau ua cov txheej txheem no.
Qhov txawv ntawm Elisa thiab Western Blot yog dab tsi?
ELISA yog ib txoj hauv kev yooj yim heev thiab zoo heev uas kuaj pom muaj cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob hauv cov neeg mob cov ntshav, thaum sab hnub poob blot yog cov txheej txheem uas kuaj pom cov protein tshwj xeeb los ntawm cov protein sib xyaw. Qhov no yog qhov sib txawv tseem ceeb ntawm Elisa thiab western blot. Elisa yog qualitative as well as quantitative. Nyob rau hauv sib piv, western blot yog qualitative. Qee lub sij hawm nws yog semi-quantitative. Thaum xav txog lub sij hawm coj mus kuaj, Elisa xeem yog lub sij hawm siv thaum lub sij hawm western blot siv sij hawm ntau dua Elisa.
Cov duab hauv qab no qhia txog qhov sib txawv ntawm Elisa thiab sab hnub poob blot.
Summary – Elisa vs Western Blot
ELISA yog ib txoj hauv kev rhiab heev thiab sophisticated uas kuaj pom muaj antigen thiab tshuaj tiv thaiv kab mob hauv peb cov ntshav. Ntawm qhov tod tes, Western blotting txheej txheem yog tsim los txheeb xyuas cov protein tshwj xeeb los ntawm cov protein sib xyaw. Qhov no yog qhov sib txawv tseem ceeb ntawm Elisa thiab Western blot. ELISA yog thawj qhov kev sim tsim los kuaj pom tus kab mob HIV. Txhawm rau kom paub meej cov txiaj ntsig ntawm ELISA xeem, Western Blot tuaj yeem siv. Ob leeg ELISA thiab Western Blot yog suav tias yog txoj hauv kev tsis ncaj rau kev kuaj pom tus kab mob HIV.
