Kinase vs Phosphatase
Qhov txawv ntawm Kinase thiab Phosphatase tshwm sim los ntawm qhov tseeb tias ob lub enzymes txhawb nqa ob txoj haujlwm sib txawv. Kinase thiab phosphatase yog ob lub enzymes tseem ceeb uas cuam tshuam nrog phosphates pom hauv cov kab mob lom. Enzymes yog peb-dimensional globular proteins uas ua raws li cov khoom siv roj ntsha rau ntau cov tshuaj biochemical hauv cov hlwb. Vim tias muaj peev xwm no, qhov tuaj txog ntawm enzymes tau suav tias yog ib qho ntawm cov xwm txheej tseem ceeb tshaj plaws hauv kev hloov pauv ntawm lub neej. Enzymes nce tus nqi ntawm cov tshuaj tiv thaiv biochemical los ntawm kev ntxhov siab rau cov tshuaj lom neeg tshwj xeeb. Kinase thiab Phosphatase yog ob qho tseem ceeb enzymes koom nrog hauv cov protein phosphorylation. Proteins phosphorylation pab txhawb kev ua haujlwm tseem ceeb ntawm cov protein, nrog rau cov cellular metabolism, kev sib txawv ntawm tes, cov teeb liab hloov thaum lub sij hawm loj hlob, transcription, tiv thaiv kab mob, thiab lwm yam. Protein phosphorylation koom nrog cov kev hloov pauv ntawm cov protein molecules los ntawm kev ntxiv cov phosphate pawg los ntawm ATP molecules, whereas dephosphorylation. Kev tshem tawm cov phosphate pawg los ntawm cov protein. Cov txheej txheem phosphorylation thiab dephosphorylation yog catalyzed los ntawm kinase thiab phosphatase enzymes feem. Hauv kab lus no, qhov sib txawv ntawm kinase thiab phosphatase yuav tau tham los ntawm kev qhia txog qhov tseeb tseem ceeb ntawm kinase thiab phosphatase.
Kinase yog dab tsi?
Kinases yog pab pawg ntawm cov enzymes uas ua rau cov tshuaj tiv thaiv phosphorylation los ntawm kev sib ntxiv ntawm phosphate pawg los ntawm ATP rau cov protein molecules. Cov tshuaj tiv thaiv phosphorylation yog unidirectional vim yog lub zog loj tso tawm nrog kev tawg ntawm phosphate-phosphate daim ntawv cog lus hauv ATP los tsim ADP. Muaj ntau ntau hom protein kinases thiab txhua kinase yog lub luag haujlwm rau phosphorylating ntawm cov protein tshwj xeeb lossis cov proteins. Txawm li cas los xij, thaum xav txog cov qauv amino acid feem ntau, kinases tuaj yeem ntxiv phosphate pawg rau peb hom amino acids, uas muaj OH pawg ua ib feem ntawm lawv pawg R. Peb cov amino acids no yog serine, threonine, thiab tyrosine. Protein kinases yog categorized raws li peb cov amino acid substrates. Serine / threonine kinase chav kawm zoo li cov protein ntau cytoplasmic. Thaum lub sij hawm txheej txheem ntawm phosphorylation, kinase tuaj yeem txhim kho lossis txo cov protein ua haujlwm. Txawm li cas los xij, kev ua haujlwm nyob ntawm qhov chaw ntawm phosphorylation thiab cov qauv ntawm cov protein ua phosphorylated.
Basic Phosphorylation cov tshuaj tiv thaiv
Phosphatase yog dab tsi?
Cov tshuaj tiv thaiv rov qab ntawm phosphorylation, dephosphorylation yog catalyzed los ntawm phosphatase enzymes. Thaum lub sij hawm dephosphorylation, phosphatase tshem tawm cov phosphate pawg los ntawm cov protein molecules. Yog li ntawd, ib qho protein ua haujlwm los ntawm kinase tuaj yeem ua kom tsis muaj zog los ntawm phosphatase. Txawm li cas los xij, cov tshuaj tiv thaiv dephosphorylation tsis thim rov qab. Muaj ntau ntau phosphatases uas muaj nyob rau hauv cov hlwb. Qee cov phosphatase yog qhov tshwj xeeb thiab dephosphorylates ib lossis ob peb cov proteins, thaum lwm tus tshem phosphate hauv ntau cov proteins. Phosphates yog hydrolases thaum lawv siv cov dej molecule rau dephosphorylation. Raws li cov substrate tshwj xeeb, phosphatase tuaj yeem muab faib ua tsib chav kawm xws li; tyrosine-specific phosphatase, serine / threonine tshwj xeeb phosphatase, dual tshwj xeeb phosphatase, histidine phosphatase, thiab lipid phosphatase.
Mechanism ntawm Tyrosine dephosphorylation los ntawm CDP
Qhov txawv ntawm Kinase thiab Phosphatase yog dab tsi?
• Kinase enzymes catalyze phosphorylation ntawm cov proteins los ntawm kev sib ntxiv ntawm phosphate pawg los ntawm ATP molecules. Phosphatase enzymes catalyze dephosphorylation cov tshuaj tiv thaiv los ntawm kev tshem tawm cov phosphate pawg los ntawm cov proteins.
• Kinase siv ATP kom tau cov phosphate pawg, whereas phosphatase siv dej molecules los tshem tawm phosphate pawg.
• Proteins uas qhib los ntawm kinase tuaj yeem ua rau deactivated los ntawm phosphatase.
