Qhov sib txawv tseem ceeb ntawm kev pom cov membrane thiab ncaj qha inoculation yog cov membrane filtration yog ib qho kev kuaj kom tsis muaj menyuam uas yuav tsum tau kuaj cov qauv ua ntej dhau los ntawm cov txheej txheem membrane uas muaj peev xwm khaws cov kab mob hauv cov kab mob hauv cov kab mob ncaj qha yog qhov kev kuaj tsis muaj menyuam uas yuav tsum tau ua. direct inoculation ntawm cov khoom xeem rau hauv cov raj lossis lub raj mis uas muaj qhov nruab nrab tsim nyog.
Muaj ntau hom kev ntsuas kev nyab xeeb, suav nrog kev kuaj tsis muaj menyuam, kuaj tshuaj lom thiab kuaj kab mob endotoxin. Kev kuaj Sterility yog ua tiav txhawm rau txheeb xyuas seb cov khoom lag luam puas tsis muaj kab mob los yog kab mob. Yog li ntawd, nws yog ib qho yuav tsum tau rau cov tshuaj tsis muaj menyuam, cov khoom siv kho mob thiab cov khoom siv kom ntseeg tau tias lawv muaj kev nyab xeeb rau kev siv. Membrane filtration thiab direct inoculation yog ob hom kev kuaj sterility.
Membrane Lim yog dab tsi?
Membrane filtration yog ib qho kev kuaj tsis muaj menyuam uas ntsuas kev nyab xeeb ntawm cov tshuaj thiab lwm yam khoom siv kho mob. Nws yog txoj kev tswj hwm kev xaiv rau cov khoom siv tshuaj lim dej. Nyob rau hauv txoj kev no, ob qho piv txwv ntawm qhov sib npaug ntawm qhov sib npaug yog cais lim los ntawm cov lim dej tsis huv. Qhov 0.45 µm qhov pores nyob rau hauv daim nyias nyias lim txwv kev lim dej ntawm cov kab mob uas muaj nyob hauv cov qauv. Tom qab ntawd cov daim nyias nyias tau muab tso rau hauv ob hom xov xwm txhawm rau txhawm rau kuaj cov kab mob aerobic nrog rau cov kab mob fungi thiab anaerobic microorganisms.
Tom qab 14 hnub incubation lub sij hawm, cov xov xwm raug soj ntsuam thiab soj ntsuam rau kev loj hlob ntawm cov kab mob. Nws yog ib qho tsim nyog los soj ntsuam cov qauv ua ntu zus; Thaum kawg ntawm lub sijhawm incubation, kev soj ntsuam zaum kawg tuaj yeem kuaj xyuas cov pov thawj ntawm kev sib kis.
Direct Inoculation yog dab tsi?
Direct inoculation yog txheej txheem kuaj tsis muaj menyuam uas ntsuas kev nyab xeeb ntawm cov tshuaj thiab lwm yam khoom. Nyob rau hauv txoj kev no, cov qauv kab lus yog ncaj qha inoculated rau hauv ob hom xov xwm. Ib qho nruab nrab tso cai rau kev loj hlob ntawm anaerobes thaum lwm qhov nruab nrab txhawb kev loj hlob ntawm aerobes. Cov kua dej thioglycollate nruab nrab yog qhov feem ntau siv nruab nrab rau anaerobes thaum tryptic soy broth yog ib qho nruab nrab siv rau aerobes. Tom qab ntawd cov kab mob aerobic thiab anaerobic tau kuaj pom nyob rau hauv ob qho tib si xov xwm thaum kawg ntawm lub sijhawm incubation. Feem ntau, inoculated media yog incubated rau 14 hnub, thiab kev soj ntsuam tsis tu ncua yog coj los xyuas kom meej qhov kev loj hlob ntawm cov kab mob.
Dab tsi yog qhov zoo sib xws ntawm Membrane Lim thiab Direct Inoculation?
- Kev pom cov membrane thiab ncaj qha inoculation yog ob hom kev kuaj tsis muaj menyuam ntawm cov tshuaj thiab lwm yam khoom siv kho mob thiab cov khoom siv.
- Ob txoj hauv kev kuaj pom cov kab mob aerobic thiab anaerobic microorganisms.
- Ob hom xov xwm yog siv los inoculate nyob rau hauv ob txoj kev.
Qhov txawv ntawm Membrane Lim thiab Direct Inoculation yog dab tsi?
Membrane filtration yog ib qho kev sim uas cov qauv kuaj tau dhau los ntawm cov kab mob sterilized thiab ces inoculated rau hauv xov xwm rau kev loj hlob ntawm cov kab mob. Nyob rau hauv sib piv, ncaj inoculation yog ib qho kev sim uas cov qauv kuaj tau ncaj qha inoculated rau hauv xov xwm thiaj li saib xyuas kev loj hlob ntawm cov kab mob. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev pom cov membrane thiab ncaj qha inoculation.
Ntxiv mus, membrane filtration yuav tsum tau lub membrane lim tsev, tab sis ncaj qha inoculation tsis xav tau ib lub lim membrane. Nyob rau hauv daim nyias nyias, cov membranes yog incubated nrog media thaum nyob rau hauv ncaj qha inoculation, cov qauv yog ncaj qha inoculated rau hauv xov xwm.
Hauv qab infographic tabulates qhov sib txawv ntawm daim nyias nyias pom thiab ncaj qha inoculation.
Summary – Membrane Filtration vs Direct Inoculation
Kev pom cov membrane thiab ncaj qha inoculation yog ob hom kev ntsuas kom tsis muaj menyuam uas ntsuas kev sib kis hauv cov khoom kho mob, suav nrog cov tshuaj. Ob txoj kev pab txhawb kev tshawb pom ntawm aerobic thiab anaerobic microorganisms. Membrane filtration siv lub tshuab lim membrane kom khaws cov kab mob nyob hauv cov qauv kab lus. Tom qab ntawd cov membranes yog incubated nyob rau hauv ob hom xov xwm. Nyob rau hauv ncaj qha inoculation, piv txwv tsab xov xwm yog ncaj qha inoculated rau hauv ob lub xov xwm yam tsis tau siv lub membrane lim tsev. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm daim nyias nyias filtration thiab direct inoculation.
