Key Difference – RNASE A vs RNASE H
Cov ribonucleases yog nucleases uas tshwj xeeb degrade RNA rau hauv me me. Lawv tuaj yeem muab faib ua ob pawg; endoribonucleases thiab exoribonucleases. Endoribonuclease yog ib qho endonuclease uas tuaj yeem degrade ib leeg stranded lossis ob stranded RNA. Nws cleaves phosphodiester bonds nyob rau hauv ib tug RNA polynucleotide saw. Cov piv txwv yog RNase A, RNase III, RNase T1, RNase P thiab RNase H. Exoribonuclease yog ib qho exonuclease uas degraded RNA los ntawm kev tshem tawm cov terminal nucleotides los ntawm 5'end lossis 3'kum ntawm RNA molecule. Cov piv txwv yog RNase R, RNase II, RNase D thiab RNase PH. Qhov sib txawv tseem ceeb ntawm RNASE A thiab RNASE H yog tias RNase A yog pancreatic ribonuclease uas tshwj xeeb degrades ib leeg-stranded RNA rau hauv cov khoom me me thaum RNase H yog ib qho tsis tshwj xeeb enzyme uas cleaves RNA hauv RNA-DNA hybrid rau hauv me me los ntawm Cov txheej txheem hydrolytic.
RNASE A yog dab tsi?
RNase A yog pancreatic ribonuclease uas tshwj xeeb cleaves unpaired cytosine thiab uracil residues ntawm 3' kawg ntawm ib leeg-stranded RNA. RNase H ua haujlwm ntawm cov ntsev ntau dua (0.3M lossis siab dua NaCl concentration). Cov tshuaj tiv thaiv hydrolytic yog ob kauj ruam. Nws tsim 3 'phosphorylated khoom ntawm 2', 3' cyclic monophosphate intermediate. Txawm hais tias nws yog qhov tshwj xeeb rau ib leeg-stranded RNA ntawm cov ntsev ntau dua, nws tseem tuaj yeem degrade ob-stranded RNA thiab RNA hauv RNA-DNA hybrid ntawm qis NaCl concentration (qis dua 0.3M NaCl).
Bruce Merrifield thawj zaug tsim cov enzyme no. RNase A yog ib qho enzyme nrov heev hauv kev tshawb fawb molecular. Bovine pancreatic RNase A yog ib qho piv txwv ntawm RNase A. Thiab nws yog ib qho ntawm cov enzymes hardiest uas tau siv hauv chav kuaj. Cov enzyme no tsis xav tau cofactor rau nws cov haujlwm. Nws yog ib qho enzyme uas thermostable heev. RNase A yog thawj enzyme thiab cov protein thib peb uas tau kuaj pom qhov tseeb amino acid. Cov enzyme no me heev nrog 124 amino acids thiab molecular loj ntawm 12600da. Thiab nws muaj plaub Histidine residues (Nws 12 thiab Nws 119 uas koom nrog hauv cov tshuaj tiv thaiv catalytic).
Daim duab 01: RNase A
RNase A tuaj yeem raug cais tawm los ntawm kev npau npau npau npau. Thaum boils, tag nrho lwm cov enzymes degrade seem RNase A. RNase A yog ib qho amazingly ruaj khov enzymes. Qhov no enzyme inhibits nrog ribonuclease inhibitor protein, hnyav hlau thiab uridine vanadate complexes.
RNase H yog dab tsi?
RNase H yog ib qho tsis tshwj xeeb ribonuclease enzyme uas tuaj yeem degrade RNA hauv RNA-DNA hybrid ntawm hydrolytic cov tshuaj tiv thaiv. RNase H cleaves 3'- O-P daim ntawv cog lus ntawm RNA hauv RNA-DNA hybrid. Thaum kawg nws tsim 3'OH thiab 5' phosphate txiav cov khoom. Hauv DNA replication nws ua lub luag haujlwm tseem ceeb los ntawm kev tshem RNA primer tom qab tsim DNA tshiab. Hauv kev kuaj mob, nws tshwj xeeb degrades RNA hauv RNA-DNA hybrid tab sis tsis yog DNA thiab unhybridized RNA. Thiab cov enzyme no feem ntau yog siv los rhuav tshem RNA qauv tom qab thawj zaug ntxiv DNA strand yog tsim thaum cDNA synthesis.
Daim duab 02: RNase H
RNase H kuj tseem siv hauv kev tshuaj xyuas nuclease tiv thaiv. Nws kuj tseem tuaj yeem suav nrog rau kev tshem tawm ntawm poly A tail los ntawm mRNA. RNase H muaj peev xwm ua kom puas qhov tsis-coding RNA sab hauv thiab sab nraud ntawm tes. Cov hlau ions yuav tsum tau ua cov cofactors rau cov haujlwm protein no. Ib qho chelator (EDTA) tuaj yeem siv los tiv thaiv RNase H enzyme.
Qhov Zoo sib xws ntawm RNASE A thiab RNASE H yog dab tsi?
- Ob leeg yog cov protein hauv cov xwm txheej.
- Ob leeg yog endoribonuclease
- Ob leeg tuaj yeem degrade RNA.
- Ob leeg ua cov tshuaj tiv thaiv hydrolytic.
- Molecular Laboratories siv ob qho no.
Qhov txawv ntawm RNASE A thiab RNASE H yog dab tsi?
RNASE A vs RNASE H |
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| RNase A yog pancreatic ribonuclease uas tshwj xeeb cleaves 3' kawg ntawm unpaired cytosine thiab uracil residues ntawm ib leeg-stranded RNA ntawm ntsev ntau dua. | RNase H yog ib qho tsis tshwj xeeb ribonuclease enzyme uas tuaj yeem degrade RNA hauv RNA-DNA hybrid ntawm hydrolytic cov tshuaj tiv thaiv. |
| Nature of RNA Cleaving | |
| RNase Ib qho tshwj xeeb cleaves ib leeg-stranded RNA ntawm ntsev ntau dua. | RNase H cleaves RNA hauv RNA- DNA hybrid. |
| Yuav tsum muaj kev sib koom ua ke rau kev ua haujlwm | |
| RNase A tsis xav tau cofactors rau nws txoj haujlwm. | RNase H xav tau hlau ions ua cofactors rau nws cov haujlwm. |
| Inhibitors of Protein Activity | |
| Ribonuclease inhibitor protein, hnyav hlau thiab uridine vanadate complexes inhibits RNAse A kev ua haujlwm. | A chelator (EDTA) inhibits RNase H kev ua haujlwm. |
| RNA Primer Tshem tawm hauv DNA Replication | |
| RNase A tsis yog siv rau RNA primer tshem tawm hauv DNA replication. | RNase H yog siv rau RNA primer tshem tawm hauv DNA replication |
| DNA Template Tshem tawm hauv Kev Sib Txuas DNA (C-DNA) Synthesis | |
| RNase A tsis yog siv rau RNA template tshem tawm thaum lub sij hawm ntxiv DNA (C-DNA) synthesis. | RNase H yog siv rau RNA template tshem tawm thaum lub sij hawm ntxiv DNA (C-DNA) synthesis. |
| Kev tshem tawm ntawm Poly-A-Tail hauv mRNA Hybridized rau Oligo (dt) | |
| RNase A tsis yog siv los tshem tawm "poly-A tail" hauv mRNA hybridized rau oligo (dt). | RNase H yog siv los tshem tawm "poly-A tail" hauv mRNA hybridized rau oligo (dt) |
Summary – RNASE A vs RNASE H
Cov ribonucleases yog cov enzymes nuclease uas muaj peev xwm tshem RNA rau hauv me me. Lawv yog ob hom; endoribonucleases thiab exoribonucleases. Endoribonuclease yog ib qho endonuclease uas muaj peev xwm degrade ib leeg-stranded los yog ob-stranded RNA. Thiab nws cleaves phosphodiester daim ntawv cog lus nyob rau hauv ib tug RNA polynucleotide saw. RNase A thiab RNase H yog ob hom endoribonucleases. RNase A yog pancreatic ribonuclease uas tshwj xeeb cleaves unpaired cytosine thiab uracil residues ntawm 3' kawg ntawm ib leeg-stranded RNA ntawm ntsev ntau dua. RNase H yog ib qho nonspecific ribonuclease enzyme uas tuaj yeem degrade RNA hauv RNA-DNA hybrid ntawm hydrolytic cov tshuaj tiv thaiv. Qhov no yog qhov txawv ntawm RNase A thiab RNase H.
Download the PDF RNASE A vs RNASE H
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm RNASE A thiab RNASE H
