Qhov Tseem Ceeb Qhov txawv - Thawj Vs Tus Kheej Thib Ob
Cov tshuaj tiv thaiv yog Y zoo li cov proteins lossis immunoglobulins tsim los ntawm cov plasma hlwb. Antibodies muaj peev xwm txheeb xyuas cov antigens, uas yog txawv teb chaws molecules xws li cov kab mob, co toxins, thiab lwm yam, thiab ua tiav cov kev hem thawj los ntawm lawv. Cov qauv tshuaj tiv thaiv yog tsim los ntawm ib feem hu ua paratope (antigen binding site tam sim no nyob rau hauv lub ntsis ntawm lub 'Y' zoo li tus qauv) los txheeb xyuas thiab khi rau lub complementary qauv ntawm cov antigen uas yog hu ua ib tug epitope. Paratope thiab epitope ua haujlwm li 'xauv' thiab 'tus yuam sij,' feem. Qhov no tso cai rau kev khi zoo ntawm cov tshuaj tiv thaiv rau cov tshuaj tiv thaiv. Cov nyhuv los ntawm antigen yog ncaj qha proportional rau hom antigen. Thaum cov tshuaj tiv thaiv kab mob khi rau ib qho antigen, nws ua rau lwm cov tshuaj tiv thaiv kab mob xws li kev ua ntawm macrophages los rhuav tshem cov kab mob txawv teb chaws. Rau kev ua kom muaj zog, cov tshuaj tiv thaiv kab mob sib txuas lus nrog lwm cov khoom ntawm lub cev tiv thaiv kab mob los ntawm cheeb tsam Fc tam sim no nyob rau hauv lub hauv paus ntawm 'Y' zoo li tus qauv ntawm cov tshuaj tiv thaiv. Muaj tsib hom tshuaj tiv thaiv: IgM, IgG, IgA, IgD, thiab IgE. Raws li cov txheej txheem ntawm kev khi ntawm cov tshuaj tiv thaiv rau ib qho antigen (ncaj qha lossis tsis ncaj), muaj ob hom tshuaj tiv thaiv kab mob hu ua thawj cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv thib ob. Thawj cov tshuaj tiv thaiv muaj peev xwm khi ncaj qha rau cov tshuaj tiv thaiv thaum lub sij hawm cov tshuaj tiv thaiv thib ob tsis khi rau cov tshuaj tiv thaiv ncaj qha tab sis cuam tshuam los ntawm kev khi rau thawj cov tshuaj tiv thaiv. Qhov no yog qhov sib txawv tseem ceeb ntawm thawj thiab theem nrab cov tshuaj tiv thaiv kab mob.
Primary Antibody yog dab tsi?
Ib hom tshuaj tiv thaiv thawj zaug tuaj yeem txhais tau tias yog immunoglobulin uas tshwj xeeb khi rau cov protein. Nws yog cov tshuaj tiv thaiv uas khi ncaj qha rau cov antigen. Qhov no tau ua tiav los ntawm kev lees paub ntawm ib qho epitope tam sim no ntawm cov antigen los ntawm thaj tsam sib txawv ntawm thawj cov tshuaj tiv thaiv. Lawv raug tsim los ua polyclonal thiab monoclonal antibodies. Cov tshuaj tiv thaiv no muaj txiaj ntsig zoo rau kev tshawb fawb los tshawb xyuas biomarkers rau cov kab mob xws li ntshav qab zib, mob qog noj ntshav, Alzheimer's kab mob thiab Parkinson's disease. Ib qho fluorophore lossis ib qho enzyme tsis muaj nyob rau hauv thawj cov tshuaj tiv thaiv.
Daim duab 01: Thawj Antibody
Yuav kom tus kws tshawb fawb pom cov tshuaj tiv thaiv, nws yuav tsum tau ua ke nrog cov tshuaj ntxiv ntxiv xws li cov tshuaj tiv thaiv kab mob thib ob. Nws tseem yog ib qho tseem ceeb los kawm txog kev nqus, kev faib tawm, cov metabolism, thiab excretion (ADME) thiab ntau yam tshuaj tiv thaiv (MDR) ntawm ntau cov tshuaj kho mob. Thawj cov tshuaj tiv thaiv muaj nyob rau hauv ntau yam ntaub ntawv uas muaj los ntawm crude antiserum mus rau antigen-purified npaj; yog li lawv tau tsim thiab muab raws li. Kev lag luam muaj cov tshuaj tiv thaiv kab mob feem ntau yog biotin sau los yog fluorescently sau npe.
Dab tsi yog Secondary Antibody?
Cov tshuaj tiv thaiv kab mob thib ob raug khi rau cov hlua hnyav ntawm cov tshuaj tiv thaiv thawj zaug los pab tshawb nrhiav, txheeb xyuas, thiab ua kom huv ntawm lub hom phiaj antigens. Cov tshuaj tiv thaiv thib ob tsis cuam tshuam nrog cov txheej txheem khi ntawm cov tshuaj tiv thaiv thawj zaug nrog cov antigens. Nws tsis khi ncaj qha rau cov antigen. Thaum thawj cov tshuaj tiv thaiv kab mob raug khi ncaj qha mus rau lub hom phiaj antigens, cov tshuaj tiv thaiv kab mob thib ob tuaj thiab khi rau thawj cov tshuaj tiv thaiv. Cov tshuaj tiv thaiv thib ob yuav tsum yog tshwj xeeb rau cov hom tshuaj tiv thaiv kab mob thiab rau cov isotope ntawm thawj cov tshuaj tiv thaiv thaum lub hom phiaj tshawb pom antigen. Hom tshuaj tiv thaiv kab mob thib ob yog xaiv los ntawm thawj cov tshuaj tiv thaiv kab mob, tus tswv tsev, thiab daim ntawv nyiam. Feem ntau cov thawj cov tshuaj tiv thaiv kab mob yog cov chav kawm IgG.
Daim duab 02: Secondary Antibody
Rau lub hom phiaj kev tshawb fawb, cov tshuaj tiv thaiv kab mob thib ob yog siv ntau hom kev soj ntsuam xws li ELISA lossis Western blotting, Flow Cytometry, thiab Immunohistochemistry, thiab lwm yam.
Dab tsi yog qhov zoo sib xws ntawm Thawj thiab Thib Ob Antibody?
- Cov tshuaj tiv thaiv thawj thiab theem nrab muaj kev cuam tshuam hauv kev tiv thaiv kab mob.
- Ob leeg sib koom cov qauv ntawm cov tshuaj tiv thaiv kab mob.
Dab tsi yog qhov txawv ntawm Thawj thiab Thib Ob Antibody?
Primary vs Secondary Antibody |
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| Primary antibody yog immunoglobulin uas tshwj xeeb khi rau ib qho protein lossis lwm yam biomolecule ntawm kev tshawb fawb txaus siab rau lub hom phiaj ntawm kev ua kom huv lossis kuaj pom thiab ntsuas. | Cov tshuaj tiv thaiv thib ob yog ib hom tshuaj tiv thaiv kab mob uas khi ncaj qha nrog cov tshuaj tiv thaiv los ntawm kev khi nrog cov tshuaj tiv thaiv thawj zaug los pab tshawb nrhiav, txheeb xyuas, thiab ua kom huv ntawm lub hom phiaj antigens. |
| Kev sib cuam tshuam nrog Antigen | |
| Primary antibodies khi ncaj qha rau cov antigen. | Cov tshuaj tiv thaiv thib ob tsis khi ncaj qha rau cov antigen tab sis cuam tshuam los ntawm kev khi nrog thawj cov tshuaj tiv thaiv. |
| Cov tshuaj tiv thaiv tseem ceeb yog siv los ua biomarkers rau kev kuaj xyuas cov kab mob xws li mob qog noj ntshav, ntshav qab zib, Alzheimer's kab mob, Parkinsons's kab mob, thiab lwm yam. | Cov tshuaj tiv thaiv thib ob yog siv hauv kev tiv thaiv kab mob. |
Summary – Primary vs Secondary Antibody
Antibodies yog immunoglobulins uas ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv kab mob. Lawv muaj 'Y' zoo li tus qauv thiab txheeb xyuas cov khoom txawv teb chaws; antigens, txhawm rau txheeb xyuas qhov muaj cov kab mob pathogenic thiab ua tiav tshem tawm lawv yam tsis pub cov kab mob ua rau muaj kev phom sij rau tus tswv tsev. Antibodies muaj tsib yam sib txawv; IgM, IgG, IgA, IgD, thiab IgE thiab raws li hom kev khi ntawm cov tshuaj tiv thaiv kab mob nrog cov tshuaj tiv thaiv (ncaj qha lossis tsis ncaj), cov tshuaj tiv thaiv muaj ob hom; thawj antibody thiab Secondary antibody. Thawj cov tshuaj tiv thaiv muaj peev xwm khi rau cov tshuaj tiv thaiv ncaj qha thaum cov tshuaj tiv thaiv thib ob tsis khi rau cov tshuaj tiv thaiv ncaj qha tab sis tsim kev sib cuam tshuam los ntawm kev khi rau thawj cov tshuaj tiv thaiv. Qhov no yog qhov txawv ntawm thawj thiab theem nrab cov tshuaj tiv thaiv kab mob.
Download PDF Version of Primary vs Secondary Antibody
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv nruab nrab ntawm Thawj thiab Secondary Antibody.
