Qhov sib txawv tseem ceeb ntawm colorimetry thiab spectrophotometric yog tias colorimetry siv cov wavelengths ruaj khov uas tsuas yog nyob rau hauv qhov pom ntau yam thaum spectrophotometry tuaj yeem siv wavelengths nyob rau hauv ntau yam.
Spectrophotometric thiab colorimetry yog cov txheej txheem peb tuaj yeem siv los txheeb xyuas cov molecules nyob ntawm lawv qhov nqus thiab emission zog. Ntxiv mus, qhov no yog ib qho yooj yim txheej txheem los txiav txim siab concentration ntawm cov qauv uas muaj xim. Txawm hais tias molecules tsis muaj xim, yog tias peb tuaj yeem ua cov xim sib xyaw los ntawm nws los ntawm cov tshuaj tiv thaiv, cov khoom sib xyaw no tuaj yeem siv rau hauv cov tswv yim no. Tsis tas li ntawd, lub zog muaj feem cuam tshuam nrog lub molecule, thiab lawv yog discrete. Yog li ntawd, kev hloov pauv tsis tu ncua ntawm lub xeev lub zog tsuas yog tshwm sim ntawm qee lub zog tsis sib xws. Hauv cov txheej txheem no, peb ntsuas qhov nqus thiab emission tshwm sim los ntawm cov kev hloov pauv hauv lub xeev lub zog. Yog li, qhov no yog lub hauv paus ntawm tag nrho cov txheej txheem spectroscopic.
Colorimetry yog dab tsi?
Colorimetry yog cov txheej txheem uas pab txiav txim siab qhov concentration ntawm cov tshuaj muaj xim. Nws ntsuas qhov kev siv ntawm cov xim thiab hais txog qhov kev siv rau qhov concentration ntawm cov qauv. Hauv colorimetry, cov xim ntawm cov qauv piv nrog cov xim ntawm tus qauv uas cov xim paub.
Daim duab 1: Kev kuaj hauv Colorimeter
Colorimeter yog cov cuab yeej siv uas peb tuaj yeem siv los ntsuas cov qauv xim thiab muab qhov haum haum.
Spectrophotometric yog dab tsi?
Spectrophotometry yog cov txheej txheem ntawm kev ntsuas ntau npaum li cas cov tshuaj lom neeg nqus lub teeb los ntawm kev ntsuas qhov siv ntawm lub teeb raws li lub teeb ntawm lub teeb hla dhau los ntawm cov qauv kev daws teeb meem. Ntxiv mus, spectrophotometer yog qhov cuab yeej siv hauv cov txheej txheem no. Nws muaj ob qhov tseem ceeb: lub spectrometer, uas tsim lub teeb nrog ib tug xaiv xim, thiab lub photometer, uas ntsuas qhov siv ntawm lub teeb.
Daim duab 2: Spectrophotometer
Nyob rau hauv lub spectrophotometer, muaj ib tug cuvette uas peb tuaj yeem tso peb cov qauv ua kua. Cov qauv ua kua yuav muaj xim, thiab nws absorbs nws cov xim ntxiv thaum lub teeb ci dhau los ntawm nws. Cov xim siv ntawm cov qauv cuam tshuam nrog qhov concentration ntawm cov khoom hauv cov qauv. Yog li ntawd, qhov concentration tuaj yeem txiav txim siab los ntawm qhov nqus ntawm lub teeb ntawm lub wavelength muab.
Qhov txawv ntawm Colorimetry thiab Spectrophotometry yog dab tsi?
Ob leeg colorimetry thiab spectrophotometric yog qhov ntsuas kom muaj nuj nqis rau kev txiav txim siab ntawm cov khoom muaj nyob hauv cov qauv. Qhov sib txawv tseem ceeb ntawm colorimetry thiab spectrophotometry yog qhov colorimetry siv cov wavelengths ruaj khov uas tsuas yog nyob rau hauv qhov pom ntau yam thaum spectrophotometry tuaj yeem siv wavelengths nyob rau hauv ntau yam.
Ntxiv mus, qhov sib txawv tseem ceeb ntawm colorimetry thiab spectrophotometer yog qhov colorimeter quantifies xim los ntawm kev ntsuas peb lub ntsiab xim ntawm lub teeb (liab, ntsuab, xiav), whereas ib spectrophotometer ntsuas cov xim meej nyob rau hauv tib neeg pom lub teeb wavelengths. Tsis tas li ntawd, colorimeter ntsuas qhov absorbance ntawm lub teeb whereas spectrophotometer ntsuas tus nqi ntawm lub teeb uas dhau los ntawm cov qauv. Yog li, qhov no kuj yog qhov sib txawv ntawm colorimetry thiab spectrophotometric.
Summary – Colorimetry vs Spectrophotometry
Hauv luv luv, colorimetry thiab spectrophotometric yog ob txoj hauv kev uas peb tuaj yeem siv los txiav txim siab cov ntsiab lus ntawm cov khoom hauv ib qho qauv los ntawm kev ntsuas lub teeb nqus los ntawm cov qauv ntawd. Qhov sib txawv tseem ceeb ntawm colorimetry thiab spectrophotometry yog tias colorimetry siv wavelengths uas tsuas yog nyob rau hauv qhov pom ntau yam thaum spectrophotometric siv tau wavelengths nyob rau hauv ib tug dav ntau yam.
