Qhov sib txawv tseem ceeb ntawm cov kab mob gram zoo thiab cov kab mob gram tsis zoo yog cov kab mob gram zoo muaj cov kab mob peptidoglycan tuab, yog li tshwm hauv cov xim ntshav thaum cov kab mob gram tsis zoo muaj txheej nyias peptidoglycan, li no tshwm sim hauv cov xim liab tom kawg. cov txheej txheem staining.
Cov kab mob yog cov prokaryotes ubiquitous uas yog unicellular thiab microscopic. Lawv zwm rau Kingdom Monera nrog rau Archaea. Ntxiv mus, lawv muaj lub koom haum cellular yooj yim heev. Raws li txoj cai, lawv tsis muaj cov khoom siv sab hauv; nucleus thiab daim nyias nyias - khi rau lwm cov organelles. Tsis tas li ntawd, cov kab mob tuaj yeem raug cais raws li ntau tus yam ntxwv xws li cov duab, caj ces pleev, cell phab ntsa muaj pes tsawg leeg, tus naj npawb ntawm flagella, khoom noj khoom haus, biochemical tshuaj, thiab lwm yam. Gram staining yog ib qho ntawm cov txheej txheem uas feem ntau siv hauv kev txheeb xyuas thiab txheeb xyuas cov kab mob. Raws li gram staining, muaj ob pawg ntawm cov kab mob xws li kab mob gram zoo thiab gram tsis zoo. Ob pawg kab mob no sib txawv ntawm ib leeg los ntawm cov phab ntsa ntawm tes. Li no, lawv stain nyob rau hauv cov xim sib txawv thaum lub sij hawm gram staining txheej txheem.
Dab tsi yog Gram Positive Bacteria?
Gram zoo kab mob yog ib pawg ntawm cov kab mob uas stain nyob rau hauv cov xim liab doog nyob rau hauv lub grams staining txheej txheem. Yog vim li cas tom qab staining nyob rau hauv cov xim liab doog yog tias gram zoo kab mob muaj ib tug tuab peptidoglycan txheej nyob rau hauv lawv cell phab ntsa. Feem ntau, peptidoglycan txheej ntawm cov kab mob gram zoo nyob ntawm 20-80 nm thickness thiab teichoic acids muaj nyob rau ntawm nws.
Daim duab 01: Cov kab mob Gram Positive
Thick peptidoglycan txheej khaws cov thawj stain, uas yog siv lead ua violet; Yog li ntawd, tshwm nyob rau hauv ntshav los yog siv lead ua violet xim nyob rau hauv lub microscope. Txawm hais tias decolourizing tus neeg saib xyuas tshem tawm cov xim tseem ceeb, stain tsis tag tawm ntawm txheej peptidoglycan tuab. Staphylococcus, Streptococcus, Bacillus, Corynebacterium, Listeria thiab Clostridia yog ib co piv txwv ntawm cov kab mob gram zoo.
Dab tsi yog Gram Negative Bacteria?
Gram negative bacteria yog ib pawg ntawm cov kab mob uas muaj txheej nyias peptidoglycan nyob rau hauv lawv cov phab ntsa ntawm tes. Yog li ntawd, lawv tsis muaj peev xwm khaws cov khoom qub. Tus cwj pwm, gram tsis zoo cov kab mob muaj cov kab mob ntxiv hu ua daim nyias nyias, uas tsis muaj cov kab mob gram zoo. Tsis tas li ntawd, cov txheej txheem no muaj lipopolysaccharides. Tsis tas li ntawd, txawm tias muaj cov txheej txheem sab nrauv, nws degrades thaum siv cov decolorizer. Tom qab ntawd, txheej peptidoglycan yuav ntxeem tau, thiab cov siv lead ua violet stain tag nrho tawm ntawm phab ntsa ntawm tes.
Daim duab 02: Cov kab mob Gram Negative
Vim li no, cov kab mob gram tsis zoo tshwm sim hauv cov xim thib ob uas yog xim liab. Thaum muab piv nrog cov kab mob gram zoo, cov kab mob gram tsis zoo muaj peev xwm tiv taus ntau dua rau cov phab ntsa ntawm tes tsom rau cov tshuaj tua kab mob. Qhov no yog vim muaj cov kab mob sab nraud. Tsis tas li ntawd, lawv cov phab ntsa ntawm tes muaj ob txheej, thiab muaj qhov chaw periplasmic hauv phab ntsa ntawm tes. Thiab tseem, cov phab ntsa ntawm tes tsis sib npaug thiab tsis nruj dua piv rau cov kab mob gram zoo. Escherichia coli, Pseudomonas, Neisseria, Chlamydia, yog qee yam ntawm cov kab mob gram negative.
Dab tsi zoo sib xws ntawm Gram Positive thiab Gram Negative Bacteria?
- Gram Positive thiab Gram Negative Bacteria muaj lub koom haum cellular zoo sib xws.
- Tsis tas li, ob leeg yog cov kab mob prokaryotic unicellular microorganisms uas muaj cov tshuaj ntsiav.
- Thiab, lawv ob leeg muaj tib chromosome thiab muaj nyob txhua qhov chaw.
- Tsis tas li ntawd, ob qho tib si yuav muaj plasmids ua lawv cov DNA extrachromosomal.
- Dhau li ntawd, ob leeg rov tsim dua asexually los ntawm binary fission.
- Ib yam li ntawd, ob qho tib si tau hloov pauv, hloov pauv thiab sib txuas.
- Ntxiv mus, Gram Positive thiab Gram Negative Bacteria yog inhibited los ntawm cov tshuaj tua kab mob.
- Lawv cov phab ntsa cell muaj peptidoglycan.
- Thiab ob hom kab mob muaj txheej txheej (S txheej).
- Lawv teb rau cov txheej txheem staining.
- Tsis tas li ntawd, lawv ua rau tib neeg, nroj tsuag thiab tsiaj txhu.
Qhov txawv ntawm Gram Positive thiab Gram Negative Bacteria yog dab tsi?
Qhov sib txawv loj ntawm cov kab mob gram zoo thiab cov kab mob gram tsis zoo yog cov kab mob gram zoo muaj cov kab mob peptidoglycan tuab hauv lawv cov phab ntsa ntawm tes thaum cov kab mob gram tsis zoo muaj txheej nyias peptidoglycan hauv lawv cov phab ntsa ntawm tes. Sib nrug los ntawm peptidoglycan txheej, gram tsis zoo cov kab mob muaj cov kab mob sab nrauv thiab nws tsis muaj nyob hauv cov kab mob gram zoo. Yog li, qhov no kuj yog qhov sib txawv ntawm cov kab mob gram zoo thiab gram negative. Tsis tas li ntawd, cov kab mob gram tsis zoo muaj qhov chaw periplasmic thiab ob txheej hauv phab ntsa ntawm tes thaum cov kab mob gram zoo tsis muaj qhov chaw periplasmic thiab lawv muaj ib txheej txheej nruj thiab cov phab ntsa ntawm tes.
Cov lus qhia hauv qab no piav qhia ntau qhov tseeb txog qhov sib txawv ntawm cov kab mob gram zoo thiab gram negative.
Summary – Gram Positive vs Gram Negative Bacteria
Nyob ntawm cov kab mob coj mus thiab khaws cov khoom qub; Crystal violet thaum lub sij hawm gram staining, muaj ob hom kab mob uas yog gram zoo thiab gram tsis zoo. Cov kab mob Gram zoo muaj txheej txheej peptidoglycan tuab hauv phab ntsa ntawm tes thaum cov kab mob gram tsis zoo muaj txheej nyias peptidoglycan. Qhov no yog qhov sib txawv loj ntawm gram positive thiab gram negative bacteria. Vim qhov sib txawv ntawm cov phab ntsa ntawm tes, cov kab mob gram zoo stain hauv cov xim ntshav thaum cov kab mob gram tsis zoo stain hauv cov xim liab hauv cov grams staining. Tsis tas li ntawd, cov kab mob gram tsis zoo muaj cov kab mob sab nrauv thaum nws tsis nyob hauv cov kab mob gram zoo. Vim tias muaj cov kab mob sab nrauv, cov kab mob gram tsis zoo tiv thaiv cov phab ntsa ntawm tes tsom cov tshuaj tua kab mob thaum cov kab mob gram zoo raug rau lawv. Yog li, qhov no qhia txog qhov sib txawv ntawm cov kab mob gram zoo thiab gram tsis zoo.
