Qhov sib txawv tseem ceeb ntawm nag lossis daus thiab agglutination cov tshuaj tiv thaiv yog cov antigens soluble thaum nag lossis daus thaum lawv tsis soluble hauv agglutination.
Kev kho cov kab mob sib kis yog nyob ntawm lawv qhov kev kuaj mob kom raug. Antigen-antibody cov tshuaj tiv thaiv yog cov txheej txheem uas peb ntsuas antigens thiab cov tshuaj tiv thaiv. Ntawm cov tshuaj tiv thaiv antigen-antibody, cov tshuaj tiv thaiv serological yog nyob rau hauv vitro cov tshuaj tiv thaiv uas yog cov txheej txheem nrov tshaj plaws rau kev kuaj mob thiab kev txheeb xyuas cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv. Cov tshuaj tiv thaiv nag lossis daus thiab cov tshuaj tiv thaiv agglutination yog qee qhov piv txwv ntawm cov tshuaj tiv thaiv serological. Muaj qee qhov sib txawv ntawm nag lossis daus thiab agglutination tshwm sim, uas peb yuav piav qhia hauv kab lus no.
nag xob nag cua cuam tshuam dab tsi?
Cov tshuaj tiv thaiv nag xob nag cua yog kev soj ntsuam serological rau kev kuaj pom cov qib immunoglobulin los ntawm cov ntshav ntawm tus neeg mob uas muaj kab mob. Cov tshuaj tiv thaiv no tshwm sim los ntawm kev sib cuam tshuam ntawm antigens thiab cov tshuaj tiv thaiv kab mob. Cov kev sib cuam tshuam no ua rau muaj nag lossis daus uas tsim los ntawm kev sib xyaw ntawm ob lub ntsiab lus soluble; ntawm no, antigens thiab antibodies.
Daim duab 01: Daim duab qhia qhov kub hnyiab hauv qhov Immunoassay
Thaum cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv muaj nyob rau hauv qhov pom tau zoo, cov nag lossis daus tshwm sim los ntawm kev tsim cov lattices lossis cross-links. Ntxiv mus, nyob rau hauv cov kev tshwm sim no, cov antigens yog soluble molecules nrog ib tug loj loj. Nrog rau kev sib piv rau qhov rhiab heev ntawm cov tshuaj tiv thaiv no, Agglutination cov tshuaj tiv thaiv yog qhov rhiab tshaj li nag lossis daus cov tshuaj tiv thaiv vim tias ntau cov tshuaj tiv thaiv soluble thiab cov tshuaj tiv thaiv kab mob yuav tsum tsim kom muaj cov tshuaj tiv thaiv nag lossis daus. Txawm li cas los xij, nws muaj peev xwm ua rau nag lossis daus rhiab heev los ntawm kev hloov nws mus rau hauv cov tshuaj tiv thaiv agglutination. Tab sis, qhov no tuaj yeem ua tiav los ntawm kev txuas cov antigens soluble rau cov neeg nqa khoom loj, tsis muaj zog xws li erythrocytes lossis latex hlaws.
Agglutination Reactions yog dab tsi?
Kev sib xyaw ntawm cov tshuaj tiv thaiv kab mob nrog lawv cov antigens zoo sib xws rau ntawm qhov chaw xws li tsiaj cell, erythrocytes, lossis cov kab mob ua rau cov tshuaj tiv thaiv kab mob sib txuas cov kab sib txuas ua cov pom pom. Cov tshuaj tiv thaiv no yog hu ua agglutination. Cov tshuaj tiv thaiv serological no zoo ib yam li cov tshuaj tiv thaiv nag lossis daus txawm tias ob qho tib si tshwj xeeb heev nyob ntawm cov tshuaj tiv thaiv tshwj xeeb thiab cov tshuaj antigen. Yog li, cov tshuaj tiv thaiv agglutination yog cov tshuaj tiv thaiv ntawm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob uas ua rau pom pom clumping. Cov tshuaj tiv thaiv no yog qhov peb hu ua "agglutinins". Qhov tseem ceeb tshaj, qhov ntau ntawm cov tshuaj tiv thaiv kab mob inhibits cov tshuaj tiv thaiv agglutination.
Daim duab 02: Agglutination hauv Haemoglobin
Yog li, peb hu qhov kev txwv no ua "prozone phenomenon". Cov tshuaj tiv thaiv no muaj kev nkag siab ntau dua, thiab nws tshwm sim zoo thaum cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob tshwm sim hauv qhov sib npaug.
Tsis tas li ntawd, hauv cov tshuaj kho mob, cov tshuaj tiv thaiv agglutination muaj ntau daim ntawv thov. Lawv tuaj yeem siv los ntaus cov qe ntshav rau kev tso ntshav, txhawm rau txheeb xyuas cov kab mob kab mob thiab txhawm rau kuaj pom muaj cov tshuaj tiv thaiv tshwj xeeb hauv cov ntshav ntawm tus neeg mob. Agglutination yog thawj zaug siv los xyuas seb tus neeg mob puas muaj kab mob los yog tsis.
Qhov txawv ntawm nag lossis daus thiab Agglutination Reactions yog dab tsi?
Cov tshuaj tiv thaiv nag xob nag cua yog kev soj ntsuam serological rau kev kuaj pom cov qib immunoglobulin los ntawm cov ntshav ntawm tus neeg mob uas muaj kab mob. Ntawm qhov tod tes, agglutination yog kev sib xyaw ntawm cov tshuaj tiv thaiv kab mob nrog lawv cov antigens txuam rau ntawm ib qho chaw xws li tsiaj cell, erythrocytes, lossis cov kab mob, uas ua rau cov tshuaj tiv thaiv kab mob sib txuas ntawm cov khoom ua kom pom pom. Qhov no yog qhov sib txawv ntawm cov nag lossis daus thiab agglutination.
Lwm qhov txawv ntawm nag lossis daus thiab agglutination yog tias cov tshuaj tiv thaiv agglutination yog rhiab dua li nag lossis daus. Vim hais tias, ntau cov kua dej antigens thiab cov tshuaj tiv thaiv kab mob molecules yuav tsum tsim kom pom cov nag lossis daus. Txawm li cas los xij, qhov sib txawv tseem ceeb ntawm nag lossis daus thiab agglutination, ob qhov kev cuam tshuam serological, cuam tshuam rau kev solubility ntawm antigens. Nyob rau hauv cov ntaub ntawv ntawm nag lossis daus, antigens yog soluble molecules thaum nyob rau hauv cov ntaub ntawv ntawm agglutination; antigens yog loj, insoluble molecules.
Summary – nag xob nag cua vs Agglutination Reactions
Antigens thiab cov tshuaj tiv thaiv yog cov reactants loj ntawm nag lossis daus thiab agglutination tshwm sim hauv immunoassays. Qhov txawv ntawm nag lossis daus thiab agglutination cov tshuaj tiv thaiv yog cov antigens soluble nyob rau hauv cov ntaub ntawv ntawm nag lossis daus thaum lawv yog insoluble nyob rau hauv agglutination.
