Qhov sib txawv tseem ceeb ntawm Alpha thiab Beta Tubulin yog tias alpha tubulin muaj Asp-254 ntawm E-site thaum lub beta-tubulin muaj Lys-254 ntawm N-site. Tsis tas li ntawd, GTP yog ib txwm txuas rau alpha-tubulin subunit, thaum nyob rau ntawm beta-tubulin subunit, GTP yog exchangeable rau microfilaments rau polymerize.
Microtubules yog ib feem ntawm cytoskeleton ntawm eukaryotic cell cytoplasm. Yog li ntawd, microtubules muaj nyob rau hauv lub network ntawm cov protein filaments faib thoob plaws hauv lub cell muab ib tug meej cov duab rau ntawm tes thiab ua kom cov organelles nyob rau hauv qhov chaw. Ntxiv mus, lawv yog tsim los ntawm ob lub ntsiab tubulin proteins uas yog alpha thiab beta tubulins. Alpha thiab beta yog ob tsev neeg ntawm eukaryotic tubulin superfamily. Alpha thiab beta tubulin tshwm sim raws li ib tug dimer, thiab nws yog lub tsev thaiv ntawm microtubules.
Alpha Tubulin yog dab tsi?
Alpha-tubulin yog ib hom globular tubulin protein, uas yog ib feem ntawm lub tsev thaiv ntawm microtubule. Alpha-tubulin ua rau dimer nrog beta-tubulin.
Daim duab 01: Tubulin Dimer
Tsis tas li ntawd, nws qhov hnyav yog kwv yees li 55 kDa thiab muaj qhov taw tes isoelectric ntawm 4.1. Alpha tubulin muaj Asp-254 ntawm E-site thiab GTP ib txwm txuas rau nws.
Beta Tubulin yog dab tsi?
Beta-tubulin yog ib hom tubulin uas koom nrog ua dimmers hauv microtubules. Nws muaj qhov hnyav zoo sib xws thiab isoelectric point nrog cov alpha tubulin.
Daim duab 02: Beta Tubulin
Txawm li cas los xij, hauv N-site ntawm beta-tubulin, muaj Lys-254. Thiab tseem GTP thiab GDP yog pauv tau hauv beta-tubulin.
Dab tsi zoo sib xws ntawm Alpha thiab Beta Tubulin?
- Alpha thiab beta tubulins muaj alpha helices, beta sheets thiab random protein coils.
- Lawv yog cov khoom ntawm microtubules.
- Ob leeg tubulins tau txais cov ntawv isotypic.
- Kev hloov pauv tom qab kev txhais lus tuaj yeem muaj nyob rau hauv ob lub tubulins.
- Ob leeg tubulins sib koom ua ke homology.
- Lawv ob leeg muaj ib puag ncig 50 kDa.
- Alpha thiab Beta Tubulin proteins polymerize rau hauv microtubules.
- Ob khi nrog GTP.
Qhov txawv ntawm Alpha thiab Beta Tubulin yog dab tsi?
Alpha tubulin thiab beta tubulin yog ob lub proteins, uas yog cov khoom ntawm microtubules. Lawv sib piv qhov hnyav thiab cov ntsiab lus isoelectric. Txawm li cas los xij, lawv cov N-site thiab E-site txawv. Ntawm E-site ntawm alpha tubulin, muaj Asp-254. Ntawm N-site ntawm beta-tubulin, muaj Lys-254. Qhov no yog qhov sib txawv tseem ceeb ntawm alpha thiab beta tubulin. Tsis tas li ntawd, GTP yeej ib txwm txuas rau alpha tubulin thaum GTP-GDP hloov pauv ntawm yeej tubulin.
Summary – Alpha vs Beta Tubulin
Cov alpha thiab beta tubulins yog ob lub globular proteins uas ua rau alpha beta dimer hauv microtubules. Yog li ntawd, Alpha thiab beta tubulin dimer yog lub hauv paus tsev thaiv ntawm microtubules. Ntxiv mus, alpha tubulin muaj Asp-254 ntawm E-site thaum beta-tubulin muaj Lys-254 ntawm N-site. Tsis tas li ntawd, alpha thiab beta tubulin txawv ntawm GTP txuas. GTP yog ib txwm txuas rau alpha tubulin subunit, thaum nyob rau ntawm beta-tubulin subunit, GTP yog exchangeable rau microfilaments rau polymerize. Qhov no yog qhov txawv ntawm alpha thiab beta tubulin.
