Qhov sib txawv tseem ceeb ntawm ib kab thiab ob kab teeb spectrophotometer yog tias nyob rau hauv ib lub teeb spectrophotometer, tag nrho cov teeb tsis dhau los ntawm tus qauv whereas, nyob rau hauv ob chav beam spectrophotometer, lub teeb beam splits rau hauv ob qhov chaw thiab tsuas yog ib feem hla. tus qauv. Spectrophotometers yog cov cuab yeej tshuaj ntsuam xyuas siv los ntsuas qhov ntsuas hauv ib qho piv txwv uas siv lub teeb ci. Yog li, cov txheej txheem no ntsuas qhov nqus ntawm lub teeb los ntawm tus qauv.
Dab tsi yog Ib Beam Spectrophotometer?
Single beam spectrophotometer yog ib qho kev ntsuas ntsuas uas txhua lub teeb nthwv dej los ntawm lub teeb ci dhau los ntawm cov qauv. Yog li ntawd, cov kev ntsuas yog coj raws li qhov siv ntawm lub teeb ua ntej thiab tom qab lub teeb dhau los ntawm cov qauv. Cov beam ib leeg spectrophotometers no ntau compact thiab optically yooj yim dua ob chav beam spectrophotometers. Thiab cov cuab yeej no kuj tsis tshua kim.
Daim duab 01: Tib Beam Spectrophotometer
Lub rhiab heev ntawm kev kuaj pom lub teeb ci tom qab nws dhau los ntawm cov qauv yog siab vim nws siv lub teeb tsis sib cais (yog li ntawd, lub zog siab muaj nyob thoob plaws). Tib beam spectrophotometers muaj nyob rau hauv kev tsom xam ntawm pom thiab ultraviolet wavelength ranges.
Ib lub teeb pom kev zoo ib leeg ntsuas qhov concentration ntawm cov tshuaj ntsuam xyuas hauv ib qho piv txwv los ntawm kev ntsuas lub teeb uas nqus los ntawm qhov ntsuas ntawd. Ntawm no, Txoj Cai Beer Lambert los ua haujlwm. Txoj cai no hais tias qhov concentration ntawm ib qho tshuaj ntsuam xyuas yog ncaj qha proportional rau absorbance.
Dab tsi yog Ob Chav Beam Spectrophotometer?
Ob chav beam spectrophotometer yog ib qho kev ntsuas ntsuas uas lub teeb ci los ntawm lub teeb pom kev sib cais ua ob feem. Ib feem ua raws li kev siv (qhov siv beam) thaum lwm qhov feem hla los ntawm cov qauv (cov qauv beam). Raws li qhov tshwm sim, cov kab siv siv tsis dhau los ntawm tus qauv.
Daim duab 02: Txoj Kev ntawm Lub Teeb Teeb hauv Ob Chav Beam Spectrophotometer
Cov qauv nqaj tuaj yeem ntsuas qhov nqus ntawm cov qauv. Cov nqaj siv tau tuaj yeem ntsuas qhov nqus (cov qauv nqaj tuaj yeem muab piv nrog cov kab siv siv). Yog li ntawd, qhov nqus yog qhov sib piv ntawm cov qauv beam (tom qab dhau los ntawm cov qauv) thiab cov lus siv beam. Ib tug spectrophotometer muaj ib tug monochromator uas cais cov xav wavelengths los ntawm lub teeb beam. Cov kab hluav taws xob siv thiab cov qauv beam recombine ua ntej tsiv mus rau monochromator. Yog li ntawd, qhov no zam lossis them nyiaj rau cov teebmeem hluav taws xob thiab cov khoom siv hluav taws xob ntawm ob qho tib si qauv thiab cov kab lus siv, sib npaug.
Qhov txawv ntawm Ib Beam thiab Ob Chav Beam Spectrophotometer yog dab tsi?
Single Beam vs Ob Chav Beam Spectrophotometer |
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| Single beam spectrophotometer yog ib qho kev ntsuas ntsuas uas txhua lub teeb nthwv dej los ntawm lub teeb ci dhau los ntawm tus qauv. | Ob chav beam spectrophotometer yog ib qho kev ntsuas ntsuas uas lub teeb ci los ntawm lub teeb pom kev sib cais ua ob feem. |
| Teeb Beam | |
| Ib leeg beam spectrophotometer siv lub teeb tsis sib cais. | Ob chav beam spectrophotometer siv lub teeb ci uas tau faib ua ob feem ua ntej dhau los ntawm tus qauv. |
| ntsuas | |
| Cov kev ntsuas coj los ntawm ib kab spectrophotometers tsis tshua muaj dua vim tias siv ib lub teeb ci. | Cov kev ntsuas coj los ntawm ob lub beam spectrophotometers yog qhov ua tau zoo dua vim tias hluav taws xob thiab cov khoom siv hluav taws xob ntawm ob qho tib si qauv thiab cov kab siv siv sib npaug. |
Summary – Ib Beam vs Ob Chav Beam Spectrophotometer
A spectrophotometer yog ib qho cuab yeej uas tshuaj xyuas cov khoom ntawm cov tshuaj los ntawm kev soj ntsuam lub peev xwm nqus lub teeb. Muaj ob lub ntsiab ntawm spectrophotometers; ib kab teeb thiab ob kab teeb spectrophotometer. Qhov sib txawv ntawm ib lub teeb thiab ob chav beam spectrophotometer yog tias, nyob rau hauv ib qho beam spectrophotometer, tag nrho cov teeb tsis dhau los ntawm tus qauv whereas, nyob rau hauv ob chav beam spectrophotometer, lub teeb beam splits rau hauv ob qhov chaw thiab tsuas yog ib feem dhau los ntawm cov qauv.
